Characterization of cytoplasmic male sterility in Petunia hybrida and Zea mays. Localization and activity of cytochrome c oxidase

Bino, R.J.; Suurs, C.J.M.; Hoop, S.J. de; Neut, A. van der; Went, J.L. van; Marrewijk, G.A.M. van

doi:10.1007/bf00028599

Cited by 7 publications

(1 citation statement)

References 26 publications

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“…No significant decrease in cytochrome c oxidase activity was observed during anther development in either the fertile or the sterile line, suggesting that the decrease in cytochrome c is not caused by downregulation of the cytochrome c oxidase pathway ( Figure 7B). In contrast, Bino et al (1986) observed a lower activity of cytochrome c oxidase in CMS petunia just after the onset of cellular degradation and in CMS-S maize before degeneration of the microspores compared with their respective isonuclear fertile lines. However, the enzyme activity measured reflects the maximum activity in the presence of excess cytochrome c substrate, which may not reflect the situation in vivo.…”

Section: Decrease In Mitochondrial Cytochrome C In Sterile Anthers Prmentioning

confidence: 59%

The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release

2001

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In mammals, mitochondria have been shown to play a key intermediary role in apoptosis, a morphologically distinct form of programmed cell death (PCD), for example, through the release of cytochrome c , which activates a proteolytic enzyme cascade, resulting in specific nuclear DNA degradation and cell death. In plants, PCD is a feature of normal development, including the penultimate stage of anther development, leading to dehiscence and pollen release. However, there is little evidence that plant mitochondria are involved in PCD. In a wide range of plant species, anther and/or pollen development is disrupted in a class of mutants termed CMS (for cytoplasmic male sterility), which is associated with mutations in the mitochondrial genome. On the basis of the manifestation of a number of morphological and biochemical markers of apoptosis, we have shown that the PET1-CMS cytoplasm in sunflower causes premature PCD of the tapetal cells, which then extends to other anther tissues. These features included cell condensation, oligonucleosomal cleavage of nuclear DNA, separation of chromatin into delineated masses, and initial persistence of mitochondria. In addition, immunocytochemical analysis revealed that cytochrome c was released partially from the mitochondria into the cytosol of tapetal cells before the gross morphological changes associated with PCD. The decrease in cytochrome c content in mitochondria isolated from male sterile florets preceded a decrease in the integrity of the outer mitochondrial membrane and respiratory control ratio. Our data suggest that plant mitochondria, like mammalian mitochondria, play a key role in the induction of PCD. The tissue-specific nature of the CMS phenotype is discussed with regard to cellular respiratory demand and PCD during normal anther development.

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Section: Decrease In Mitochondrial Cytochrome C In Sterile Anthers Prmentioning

confidence: 59%

The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release

2001

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Protoplast Fusion‐Mediated Transfer of Male Sterility and Other Plasmone‐Controlled Traits

Galun

Perl

Aviv

2007

Ciba Foundation Symposium 137 ‐ Applications of Plant Cell and Tissue Culture

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Effects of Petunia cytoplasmic male sterile (CMS) cytoplasm on the development of sterile and fertility‐restored P.parodii anthers

Conley

Parthasarathy

Hanson

1994

American J of Botany

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The developmental defects causing cytoplasmic male sterility in Petunia parodii are described in isonuclear fertile, sterile, and fertility‐restored plants using both light‐ and scanning electron microscopy. The aberrant development of the sporogenous tissue and tapetal layer caused by the cytoplasmic male sterile cytoplasm in both Petunia hybrida and P. parodii nuclear backgrounds is similar in onset and progression. The degeneration of the sporogenous tissue and tapetal layer of sterile anthers is first apparent late in meiosis and results in highly abnormal sterile sporogenous tissue by tetrad stage of fertile anthers. The stomium and endothecium do not show major developmental differences between fertile and sterile anthers, but the inner connective tissue of sterile anthers contained calcium crystals not found at high abundance in fertile anthers. Ovoid bodies containing magnesium and phosphorus were seen only in the vascular bundles of fertile anthers. Material prepared for the scanning electron microscope by freeze drying showed better retention of fragile morphological features, while critical‐point drying permitted examination of nonvolatile structures, such as cell walls.

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Characterization of cytoplasmic male sterility in Petunia hybrida and Zea mays. Localization and activity of cytochrome c oxidase

Cited by 7 publications

References 26 publications

The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release

The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release

Protoplast Fusion‐Mediated Transfer of Male Sterility and Other Plasmone‐Controlled Traits

Effects of Petunia cytoplasmic male sterile (CMS) cytoplasm on the development of sterile and fertility‐restored P.parodii anthers

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