Characterization of cytohesin-1 monoclonal antibodies: Expression in neutrophils and during granulocytic maturation of HL-60 cells

Garceau, Valérie; Houle, Martin; Chouinard, François; Gagnon, Simon; Harbour, Danielle; Naccache, Paul H.; Bourgoin, Sylvain G.

doi:10.1016/s0022-1759(00)00336-7

Cited by 9 publications

(20 citation statements)

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“…HRP-labeled donkey anti-rabbit and sheep anti-mouse IgGs were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA) and from GE Healthcare, respectively. Monoclonal Arf6 (8A6-4) and cytohesin-1 (2E11) Abs were generated in mice as previously described (20). Polyclonal Arf1 and cytohesin-1 Abs were produced in rabbits as described previously (20).…”

Section: Absmentioning

confidence: 99%

“…Monoclonal Arf6 (8A6-4) and cytohesin-1 (2E11) Abs were generated in mice as previously described (20). Polyclonal Arf1 and cytohesin-1 Abs were produced in rabbits as described previously (20). Rabbit anti-human p47 phox Ab was obtained from Upstate Biotechnology (Lake Pacid, NY).…”

Section: Absmentioning

confidence: 99%

“…Cytohesins share a common domain organization consisting of an N-terminal coiled-coil domain, a central Sec7 domain (Arf-GEF activity), a phosphoinositide-binding pleckstrin homology (PH) domain, and a short C-terminal extension rich in positively charged amino acids (19). Cytohesin-1 but not ARNO expression increases with granulocytic differentiation (10,20), suggesting a potential role of cytohesin-1 in the acquisition of a granulocytic phenotype. In lymphocytes, cytohesin-1 regulates cell adhesion via b-2 integrin activation (21,22).…”

mentioning

confidence: 99%

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Cytohesin-1 Regulates the Arf6-Phospholipase D Signaling Axis in Human Neutrophils: Impact on Superoxide Anion Production and Secretion

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Garceau

Harbour

et al. 2009

The Journal of Immunology

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Polymorphonuclear neutrophil (PMN) stimulation with fMLP stimulates small G proteins such as ADP-ribosylation factors (Arfs) Arf1 and Arf6, leading to phospholipase D (PLD) activation and functions such as degranulation and the oxidative burst. However, the molecular links between fMLF receptors and PLD remain unclear. PMNs express cytohesin-1, an Arf-guanine exchange factor that activates Arfs, and its expression is strongly induced during the acquisition of the neutrophilic phenotype by neutrophil-like cells. The role of cytohesin-1 in the activation of the fMLF-Arf-PLD signaling axis, and the accomplishment of superoxide anion production, and degranulation was investigated in PMNs using the selective inhibitor of cytohesin, Sec 7 inhibitor H3 (secinH3). Cytohesin-1 inhibition with secinH3 leads to Arf6 but not Arf1 inhibition, demonstrating the specificity for Arf6, and fMLF-mediated activation of PLD and of the oxidative burst as well. We observed a decrease in fMLF-mediated protein secretion and expression of cell surface markers corresponding to primary (CD63/myeloperoxidase), secondary (CD66/lactoferrin), and tertiary (matrix metalloproteinase-9) granules in PMNs incubated with secinH3. Similarly, silencing cytohesin-1 or Arf6 in PLB-985 cells negatively affected fMLF-induced activation of PLD, superoxide production, and expression of granule markers on the cell surface. In contrast, stable overexpression of cytohesin-1 in PLB-985 cells enhanced fMLF-induced activation of Arf6, PLD, and NADPH oxidase. The results of this study provide evidence for an involvement of cytohesin-1 in the regulation of the functional responses of human PMNs and link these events, in part at least, to the activation of Arf6.

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Section: Absmentioning

confidence: 99%

Section: Absmentioning

confidence: 99%

mentioning

confidence: 99%

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Cytohesin-1 Regulates the Arf6-Phospholipase D Signaling Axis in Human Neutrophils: Impact on Superoxide Anion Production and Secretion

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Garceau

Harbour

et al. 2009

The Journal of Immunology

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“…Anti-CD14 mAb, clone 3C10, was a gift from Dr. W. C. Van Voorhis (University of Washington, Seattle, WA) and 63D3 was a gift from Dr. P. S. Tobia (The Scripps Research Institute, La Jolla, CA). Anti-cytohesin-1 mAb (clone 7H2) was a gift from Dr. W. Kolanus (Ludwig-Maximilians-Universität, München, Germany) and rabbit polyclonal anti-cytohesin-1 (O139) was previously described (22). Anti-TLR2 (clone TL2.1), anti-TLR4 (clone HTA125), and FITC-labeled anti-CR3 activation epitope (clone CBRM1/5) were from eBiosciences.…”

Section: Antibodiesmentioning

confidence: 99%

“…B, Cells were prepared as in A and stimulated for 10 min with BCG alone or serum-opsonized BCG, LAM (5 g/ml) alone, or LAM in presence of 200 ng/ml LBP. Cell samples (A and B) were assayed for PI3K activity as previously described (22,23). Spots corresponding to phosphatidylinositol phosphate (PIP) were cut and analyzed by scintillation counting.…”

Section: Mycobacterial Lam Up-regulates the Avidity Of Cr3mentioning

confidence: 99%

Cross-Talk between CD14 and Complement Receptor 3 Promotes Phagocytosis of Mycobacteria: Regulation by Phosphatidylinositol 3-Kinase and Cytohesin-1

Sendide

Reiner

Lee

et al. 2005

The Journal of Immunology

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The glycosylphosphatidyl anchored molecule CD14 to the monocyte membrane plays a prominent role in innate immunity, and the paradigms for CD14 selective signaling are beginning to be elucidated. In this study, transfected human monocytic cell line THP-1 and Chinese hamster ovary (CHO) fibroblastic cells were used to examine phagocytosis of Mycobacterium bovis bacillus Calmette-Guérin (BCG). Flow cytometry was combined with molecular and biochemical approaches to demonstrate a dual mechanism for BCG internalization involving either CD14 alone or a CD14-regulated complement receptor (CR)3-dependent pathway. Phagocytosis by CD14-positive THP-1 cells was attenuated by phosphatidylinositol-3 inhibitors LY294002 and wortmannin and experiments using transfected CHO cells showed substantial accumulation of phosphatidylinositol-3,4,5-trisphosphate at the BCG attachment site in CHO cells expressing CD14 and TLR2 suggesting that bacteria bind to CD14 and use TLR2 to initiate a PI3K signaling pathway. Additional experiments using blocking Abs showed that anti-TLR2 Abs inhibit phagocytosis of BCG by THP-1 cells. Furthermore, knockdown of cytohesin-1, a PI3K-regulated adaptor molecule for β2 integrin activation, specifically abrogated CD14-regulated CR3 ingestion of BCG consistent with the observation of physical association between CR3 and cytohesin-1 in cells stimulated with mycobacterial surface components. These findings reveal that mycobacteria promote their uptake through a process of “inside-out” signaling involving CD14, TLR2, PI3K, and cytohesin-1. This converts low avidity CR3 into an active receptor leading to increased bacterial internalization.

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Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation

Azreq

Bourgoin

2011

Molecular Immunology

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Characterization of cytohesin-1 monoclonal antibodies: Expression in neutrophils and during granulocytic maturation of HL-60 cells

Cited by 9 publications

References 50 publications

Cytohesin-1 Regulates the Arf6-Phospholipase D Signaling Axis in Human Neutrophils: Impact on Superoxide Anion Production and Secretion

Cytohesin-1 Regulates the Arf6-Phospholipase D Signaling Axis in Human Neutrophils: Impact on Superoxide Anion Production and Secretion

Cross-Talk between CD14 and Complement Receptor 3 Promotes Phagocytosis of Mycobacteria: Regulation by Phosphatidylinositol 3-Kinase and Cytohesin-1

Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation

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