Characterization of Copolymer Dehypon® LS 54 and Its Application for Aqueous Two-Phase Systems Paired with the Waxy Maize Starch for Protein Extraction

Abstract

A thermo-separating aqueous two-phase system composed of Dehypon ® LS 54, a polymeric surfactant and the waxy maize starch (amylopectin starch) has been used for partitioning of cutinase as a model protein. Dehypon ® LS 54 were characterized by using 1 H NMR spectroscopy to get information regarding the chemical structure and to confirm the presence of aliphatic moiety group in this copolymer. The phase diagram obtained for these novel polymer-polymers two-phase system shows two-phases with high polymer concentration. The waxy maize starch is enriched in the bottom phase while the copolymer Dehypon ® LS 54 is found in the upper phase. Since this copolymer (Dehypon ® LS 54) is thermo-reactive, the upper phase can be removed and heated above the copolymer's cloud-point which resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper is copolymer-rich phase. Our results show that systems formed by waxy maize starch and Dehypon ® LS 54 could become an alternative system to be used in large scale protein and enzyme purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling which makes this system more attractive.

Decellularised tissues obtained by a CO2-philic detergent and supercritical CO2

“…For instance, decellularisation of rat hearts with SDS in pulsatile flow retains most of the GAGs in the ECM (Park et al, 2018). Likewise, optimisation of the decellularisation protocol can significantly enhance the remaining GAG content in a decellularised -Jünger et al, 2016). GAGs are important for the visco-elastic properties of tissues (Lovekamp et al, 2006).…”

“…Then, the skin was immediately treated according to the section "scCO 2 treatment". scCO 2 treatment Based on the protocols described by others (Guler et al, 2017;Huang et al, 2017;Sawada et al, 2008;Wang et al, 2017), scCO 2 was used to eliminate cellular components from the tissues (Fig. 1).…”

“…ethanol, is necessary to eliminate or counteract charged molecules such as phospholipids (Dunford and Temelli, 1995;Montanari et al, 1999;Tanaka et al, 2004). Using scCO 2 with ethanol already provides successful decellularisation for aorta, cornea, oesophagus and adipose tissues (Casali et al, 2018;Guler et al, 2017;Halfwerk et al, 2018;Huang et al, 2017;Sawada et al, 2008;Wang et al, 2017;Zambon et al, 2016). However, when dealing with denser tissues, ethanol does not have the required counteractive solvent strength.…”

Laser Irradiation Effects at Different Wavelengths on Phenology and Yield Components of Pretreated Maize Seed

“…For this purpose, maize was selected as a model plant due to its significant economic value as a worldwide cultivated crop for its high starch content [31,32]. Furthermore, studies revealed that the laser is useful to improve the sanitary quality of maize seed [33]; this characteristic allows a seedling to have more strength to emerge from the soil [34].…”