Characterization of constitutive heterochromatin inCebus apella(Cebidae, Primates) andPan troglodytes (Hominidae, Primates): Comparison to human chromosomes

García, Francisca; Nogués, Carme; Garcı́a, M.; Egozcue, J.; Ponsà, M.

doi:10.1002/(sici)1098-2345(199911)49:3<205::aid-ajp1>3.0.co;2-8

Cited by 11 publications

(10 citation statements)

References 25 publications

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“…Our data and data from Pieczarka et al . (1996) and García et al . (1999) suggest that the origin of ITH is recent, a fact which could explain the homogeneity observed in nearly all the species, and that it may have occurred after speciation or was perhaps, in some cases, related to the process of speciation.…”

Section: Discussionmentioning

confidence: 99%

“…Distamycin A/DAPI was performed on unbanded chromosomes (DA/DAPI) (Schweizer, Ambros & Andrle, 1978), on C‐banded chromosomes (C + DA/DAPI) (Bella & Gosalvez, 1991) , and on sequentially G‐C banded chromosomes (G‐C + DA/DAPI) (García et al ., 1999) . In DA/DAPI stained chromosomes which had been previously G‐C banded, control of bands was made by staining half of the slide with Wright (G‐bands) or Giemsa (C‐bands).…”

Section: Methodsmentioning

confidence: 99%

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Qualitative analysis of constitutive heterochromatin and primate evolution

García

Garcı́a

Mora

et al. 2003

Biological Journal of the Linnean Society

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The results of qualitative heterochromatin analysis in 16 species of primates: Homo sapiens, Pan troglodytes and Gorilla gorilla (F. Hominidae), Hylobates syndactilus (F. Hylobatidae), Macaca fascicularis, M. tibetana, Mandrillus sphinx, M. leucophaeus, Cercopithecus aethiops, C. sabaeus and C. albogularis (F. Cercopithecidae), Cebus apella, Ateles belzebuth hybridus, Aotus azarae, Saimiri sciureus and Lagothrix lagothricha (F. Cebidae) are presented in this work. We characterized heterochromatin using: (a) in situ digestion with restriction enzymes AluI, HaeIII, RsaI and Sau3A, and (b) chromosome staining with DA/DAPI on unbanded chromosomes, on C‐banded chromosomes and on sequentially G‐C‐banded chromosomes. The aim of this work was to relate the qualitative characteristics of constitutive heterochromatin observed with the cytogenetic evolutive processes in the primate group. Results obtained show that (1) in the family Cercopithecidae, Papionini species do not present chromosomal rearrangements when their karyotypes are compared and the heterochromatin characteristics are uniform, while Cercopithecini species show a high number of chromosomal reorganizations, but they have the same heterochromatic characteristics; (2) the Platyrrhini species analysed show variability in their karyological and heterochromatic characteristics; (3) the Hominoidea present two different situations: Pan, Gorilla and Homo with few chromosomal reorganizations among their karyotypes but with a high variability in their heterochromatin characteristics, and Hylobates with low heterochromatin variability and a highly derived karyotype. Speciation processes related to chromosome changes and heterochromatin variations in different groups of primates are discussed. © 2003 The Linnean Society of London, Biological Journal of the Linnean Society, 2003, 80, 107–124.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Qualitative analysis of constitutive heterochromatin and primate evolution

García

Garcı́a

Mora

et al. 2003

Biological Journal of the Linnean Society

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“…Human (HSA) chromosome-specific probes (Oncor and Cambio) were used to hybridize CNI and ABH chromosomes following the method described in García et al [1999García et al [ , 2000.…”

Section: Zoo-fishmentioning

confidence: 99%

Chromosomal homologies between Cebus and Ateles (Primates) based on ZOO‐FISH and G‐banding comparisons

García

Ruiz‐Herrera²,

Egozcue

et al. 2002

American J Primatol

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ZOO-FISH (Fluorescent "in vitro" hybridization) was used to establish the chromosomal homology between humans (HSA) and Cebus nigrivitatus (CNI) and Ateles belzebuth hybridus (ABH). These two species belong to different New World monkey families (Cebidae and Atelidae, respectively) which differ greatly in chromosome number and in chromosome morphology. The molecular results were followed by a detailed banding analysis. The ancestral karyotype of Cebus was then determined by a comparison of in situ hybridization results, as well as chromosomal morphology and banding in other Platyrrhini species. The karyotypes of the four species belonging to the genus Cebus differ from each other by three inversions and one fusion as well as in the location and amounts of heterochromatin. Results obtained by ZOO-FISH in ABH are in general agreement with previous gene-mapping and in situ hybridization data in Ateles, which show that spider monkeys have highly derived genomes. The chromosomal rearrangements detected between HSA and ABH on a band-to-band basis were 27 fusions/fissions, 12 centromeric shifts, and six pericentric inversions. The ancestral karyotype of Cebus was then compared with that of Ateles. The rearrangements detected were 20 fusions/fissions, nine centromeric shifts, and five inversions. Atelidae species are linked by a fragmentation of chromosome 4 into three segments forming an association of 4/15, while Ateles species are linked by 13 derived associations. The results also helped clarify the content of the ancestral platyrrhine karyotype and the mode of chromosomal evolution in these primates. In particular, associations 2/16 and 5/7 should be included in the ancestral karyotype of New World monkeys.

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“…The REs induce structural alterations in metaphase chromosomes which could be related, at the cytological level, to specific structural characteristics of eukaryote chromosome bands, reflecting a distribution of different DNA sequences along the chromosomes (Fernandez et al, 1998;Garcia et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I

Faria

Morielle‐Versute

2002

Genet. Mol. Biol.

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The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band-positive regions of bat chromosomes have a different DNA composition.

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Characterization of constitutive heterochromatin inCebus apella(Cebidae, Primates) andPan troglodytes (Hominidae, Primates): Comparison to human chromosomes

Cited by 11 publications

References 25 publications

Qualitative analysis of constitutive heterochromatin and primate evolution

Qualitative analysis of constitutive heterochromatin and primate evolution

Chromosomal homologies between Cebus and Ateles (Primates) based on ZOO‐FISH and G‐banding comparisons

In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I

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