Characterization of complex chromosomal abnormalities in uveal melanoma by fluorescence in situ hybridization, spectral karyotyping, and comparative genomic hybridization

Microarray comparative genomic hybridisation analysis of intraocular uveal melanomas identifies distinctive imbalances associated with loss of chromosome 3

“…Table 2 shows the chromosomal changes for all 18 tumours analysed, and Figure 2 shows the frequency of CNAs detected at the level of each chromosome arm. The overall frequency of alterations observed in the 18 tumours was higher than has been previously reported in metaphase CGH studies of UVM (Ghazvini et al, 1996;Tschentscher et al, 2000;Aalto et al, 2001;Naus et al, 2001). This reflects the greater sensitivity of array CGH.…”

Microarray comparative genomic hybridisation analysis of intraocular uveal melanomas identifies distinctive imbalances associated with loss of chromosome 3

“…Table 2 shows the chromosomal changes for all 18 tumours analysed, and Figure 2 shows the frequency of CNAs detected at the level of each chromosome arm. The overall frequency of alterations observed in the 18 tumours was higher than has been previously reported in metaphase CGH studies of UVM (Ghazvini et al, 1996;Tschentscher et al, 2000;Aalto et al, 2001;Naus et al, 2001). This reflects the greater sensitivity of array CGH.…”

Multiple locations on chromosome 3 are the targets of specific deletions in uveal melanoma

“…10,19,24,25 The present study detected two tumours (SOM 240 and SOM 177) with specific abnormalities affecting chromosome 3q. In SOM 240, a deletion affecting 3q23-24 to 3qter was observed by cytogenetic analysis.…”

Genomic and Expression Profiling of Human Spermatocytic Seminomas: Primary Spermatocyte as Tumorigenic Precursor and DMRT1 as Candidate Chromosome 9 Gene

“…Cells were counterstained with 4V ,6-diamidino-2-phenylindole and mounted in antifade solution (Dabco-Vectashield 1:1). Using the Spectra Cube 300 system and Skyview analysis software (ASI), nine metaphase cells were examined (26).…”

Microarray comparative genomic hybridisation analysis of intraocular uveal melanomas identifies distinctive imbalances associated with loss of chromosome 3

“…Table 2 shows the chromosomal changes for all 18 tumours analysed, and Figure 2 shows the frequency of CNAs detected at the level of each chromosome arm. The overall frequency of alterations observed in the 18 tumours was higher than has been previously reported in metaphase CGH studies of UVM (Ghazvini et al, 1996;Tschentscher et al, 2000;Aalto et al, 2001;Naus et al, 2001). This reflects the greater sensitivity of array CGH.…”

Multiple locations on chromosome 3 are the targets of specific deletions in uveal melanoma

“…10,19,24,25 The present study detected two tumours (SOM 240 and SOM 177) with specific abnormalities affecting chromosome 3q. In SOM 240, a deletion affecting 3q23-24 to 3qter was observed by cytogenetic analysis.…”

Genomic and Expression Profiling of Human Spermatocytic Seminomas: Primary Spermatocyte as Tumorigenic Precursor and DMRT1 as Candidate Chromosome 9 Gene

“…Cells were counterstained with 4V ,6-diamidino-2-phenylindole and mounted in antifade solution (Dabco-Vectashield 1:1). Using the Spectra Cube 300 system and Skyview analysis software (ASI), nine metaphase cells were examined (26).…”

Microarray comparative genomic hybridisation analysis of intraocular uveal melanomas identifies distinctive imbalances associated with loss of chromosome 3

“…Table 2 shows the chromosomal changes for all 18 tumours analysed, and Figure 2 shows the frequency of CNAs detected at the level of each chromosome arm. The overall frequency of alterations observed in the 18 tumours was higher than has been previously reported in metaphase CGH studies of UVM (Ghazvini et al, 1996;Tschentscher et al, 2000;Aalto et al, 2001;Naus et al, 2001). This reflects the greater sensitivity of array CGH.…”

Multiple locations on chromosome 3 are the targets of specific deletions in uveal melanoma

“…10,19,24,25 The present study detected two tumours (SOM 240 and SOM 177) with specific abnormalities affecting chromosome 3q. In SOM 240, a deletion affecting 3q23-24 to 3qter was observed by cytogenetic analysis.…”

Genomic and Expression Profiling of Human Spermatocytic Seminomas: Primary Spermatocyte as Tumorigenic Precursor and DMRT1 as Candidate Chromosome 9 Gene

“…Cells were counterstained with 4V ,6-diamidino-2-phenylindole and mounted in antifade solution (Dabco-Vectashield 1:1). Using the Spectra Cube 300 system and Skyview analysis software (ASI), nine metaphase cells were examined (26).…”