Characterization of Coa Gene and Antimicrobial Profiles of Staphylococcus Aureus Isolated from Bovine Clinical and Subclinical mastitis

Younis, Gamal; Sadat, Asmaa; Maghawry, Mona

doi:10.17582/journal.aavs/2018/6.4.161.168

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…In addition, they were close to the results ofDaka et al [38] who reported the highest resistance of S. aureus to ampicillin followed by oxacillin, erythromycin, amoxicillin-clavulanic acid, trimethoprim/sulphamethoxazole, and no resistance was detected for ciprofloxacin. However, our results are inconsistent with a previous study in Egypt on the detection of high resistance levels against vancomycin (93.75%) [41]. Failure to respond to antibiotic therapy was found to be multifactorial in lactating cows, which may be attributed to the presence of micro-abscesses within the udder and inaccessibility of the drug to the causative agent and avoiding the effect of antibiotic by residing inside macrophages.…”

Section: Resultscontrasting

confidence: 99%

“…All the S. aureus isolates harbored the amplified products of both nuc and coa genes with characteristic bands at 395 bp and 630 bp, respectively indicating a high correlation between biochemical identification and genetic detection of these isolates (Figure 1 and 2). These findings were in close alignment with the previous study of Younis et al [41] who found that the nuc gene was detected in all of the S. aureus isolates while there was no correlation in the screening of the coa gene where 28 isolates were found positive for the coa gene despite being negative in the coagulase test. This might be explained by the unfunctionally of coa gene in these strains.…”

Section: Antimicrobial Agent Testedsupporting

confidence: 91%

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Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Talaat

Beskawy²,

Atwa³

et al. 2023

Zagazig Veterinary Journal

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Mastitis is a multifactorial and ubiquitous disease that results from interactions between the host, environment, and infectious agents leading to extensive economic losses. The objectives of this study were to estimate the prevalence of S. aureus in clinical and subclinical mastitis in Holstein dairy cows and determine the susceptibility of S. aureus isolatesagainst different antibiotics for screening of antibiotic resistance genes. A total of 415 Holstein dairy cows were randomly selected from three Egyptian governorates. Selected cows were examined for clinical and subclinical mastitis during the period from October 2014 to June 2018. Milk samples were examined for the presence of S. aureus. The S. aureus isolates were subjected to antibiotic sensitivity, molecular identification, and detection of the antibiotic resistance gene.The prevalence of S. aureus isolated from milk samples was 15.4% of which, 14.3% in clinical cases, and 15.7% in subclinical cases.The antibiogram of S. aureus isolates against 13 antibiotics using the disc-diffusion method revealed the highest rate of resistance to Oxacillin (OX) (96.7%), followed by Ampicillin (AM),Cefoxitin (FOX) (93.3%, each), Tetracycline (TE) (73.3%), Cefotaxime (CTX) (70%), Ampicillin/Sulbactam (SAM) (66.7%), Erythromycin (E) and Sulphamethoxazole/Trimethoprim (SXT) (56.7%, each), Gentamicin (GM) (53.3%), Ofloxacin (OFX) (40%), Chloramphenicol (C) (36.7%), Ciprofloxacin (CIP) (30%) and finally Vancomycin (VA) (0%). Molecular PCR assay revealed that all the 16 S. aureus isolates (100%) carried mecA gene, while 15 out of 16 isolates (93.7%) carried blaZ genebut, 8 out of 16 (50%) carried tetK gene, and only one isolate (0.06%) carried fexA gene. Uncontrolled uses of antibiotics in the treatment of mastitis should be restricted and increase awareness about the risk of antimicrobial-resistant bacteria in milk.

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Section: Resultscontrasting

confidence: 99%

Section: Antimicrobial Agent Testedsupporting

confidence: 91%

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Talaat

Beskawy²,

Atwa³

et al. 2023

Zagazig Veterinary Journal

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“…Besides, P. aeruginosa was isolated in 12.5% (8/64), 28.57% (2/7), and 1.72 (1/58) of rabbit, butter, and milk samples, respectively, which is consistent with previously published findings from the same food type [ 47 – 50 ]. Furthermore, P. mirabilis was encountered in 1.51 (1/66) and 3.45 (2/58) of chicken meat and milk samples, respectively, which was similar to previously reported rates in chicken meat [ 51 , 52 ], but higher than other previously obtained levels [ 3 , 53 ] from chicken meat and milk. Human UTIs, nosocomial infections, and wound infections have all been linked to Proteus [ 54 , 55 ].…”

Section: Discussionsupporting

confidence: 90%

Prevalence, antibiotic resistance patterns, and biofilm formation ability of Enterobacterales recovered from food of animal origin in Egypt

et al. 2023

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Background and Aim: The majority of animal-derived food safety studies have focused on foodborne zoonotic agents; however, members of the opportunistic Enterobacteriaceae (Ops) family are increasingly implicated in foodborne and public health crises due to their robust evolution of acquiring antimicrobial resistance and biofilms, consequently require thorough characterization, particularly in the Egyptian food sector. Therefore, this study aimed to determine the distribution and prevalence of Enterobacteriaceae family members in animal-derived foods, as well as their resistance to important antimicrobials and biofilm-forming potential. Materials and Methods: A total of 274 beef, rabbit meat, chicken meat, egg, butter, and milk samples were investigated for the presence of Enterobacteriaceae. All isolated strains were first recognized using traditional microbiological techniques. Following that, matrix-assisted laser desorption ionization-time of flight mass spectrometry was used to validate the Enterobacteriaceae's identity. The isolated enterobacteria strains were tested on disk diffusion and crystal violet quantitative microtiter plates to determine their antibiotic resistance and capacity to form biofilms. Results: There have been thirty isolates of Enterobacteriaceae from seven different species and four genera. Out of the three food types, Pseudomonas aeruginosa had the highest prevalence rate (4.1%). With three species, Enterobacter genera had the second-highest prevalence (3.28%) across five different food categories. In four different food types, the Klebsiella genera had the second-highest distribution and third-highest incidence (2.55%). Almost all isolates, except three Proteus mirabilis, showed prominent levels of resistance, particularly to beta-lactam antibiotics. Except for two Enterobacter cloacae and three P. mirabilis isolates, all isolates were classified as multidrug-resistant (MDR) or extensively multidrug-resistant (XDR). The multiple antibiotic resistance index (MARI) of the majority of isolates dropped between 0.273 and 0.727. The highest MARI was conferred by Klebsiella pneumoniae, at 0.727. Overall, 83.33% of the isolates had strong biofilm capacity, while only 16.67% exhibited moderate capacity. Conclusion: The MDR, XDR, and strong biofilm indicators confirmed in 83.33% of the currently tested Enterobacteriaceae from animal-derived foods suggest that, if not addressed, there may be rising risks to Egypt's economy and public health.

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“…Although less common in other mastitis research, P. mirabilis was shown to be present in several bovine and caprine mastitis cases. It was detected in dairy cows with subclinical mastitis (Jas, 2017;Younis et al, 2017). It was also detected in the milk of infected lactating sheep and goats (Housawi et al, 2008).…”

Section: Unintended Isolation Of Non-streptococci Bacterial Pathogensmentioning

confidence: 99%

Prevalence of Streptococci spp. and Unexpected Non-Streptococci Strains Associated with Bovine Mastitis Infection in Dairy Cattle in Region IV-A, Philippines

Ancuelo,

Perez

2023

MJST

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Bovine mastitis is an inflammatory response of the udder tissue in the mammary gland caused by microbial infections. Streptococcus spp. is among the most prevalent mastitis-inducing etiological agents. Thus, this study intended to isolate and evaluate the prevalence of Streptococci in dairy cattle infected with clinical mastitis in Region IV-A, Philippines. Edward Agar medium with 6% defibrinated sheep blood was employed as a selective medium. The bacterial isolates were phenotypically and genotypically characterized. Remarkably, out of 98 isolates, only 26.5% belonged to the genus Streptococcus despite the use of a Streptococci-specific medium. Five Streptococci species and 22 non-Streptococci species were identified. The most prevalent species were S. uberis (prevalence rate: 11.2%). The antimicrobial resistance profiling also revealed that S. agalactiae exhibited resistance to all antimicrobials used, while S. bovis showed hyper-resistance to five out of seven antibiotics. Surprisingly, most of the non-streptococcal isolates exhibited hyper-resistance to multiple antibiotics. For instance, Klebsiella pneumoniae isolates showed high resistance against all antimicrobials. Proteus and Providencia isolates exhibited resistance against six out of seven antibiotics. Strong hemolytic activity was also observed in Bacillus subtilis. The detection of diverse species of microorganisms causing mastitis is significant to the dairy industry as distinct pathogens may entail different risks and necessitate specific treatments, primarily in terms of the antimicrobials that will be utilized to cure the infection. Application of inappropriate antibiotics might unduly expose the udder microbial flora to antimicrobials, increasing the establishment of multidrug-resistant bacteria, which is a severe hazard to animal and human health.

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Characterization of Coa Gene and Antimicrobial Profiles of Staphylococcus Aureus Isolated from Bovine Clinical and Subclinical mastitis

Cited by 6 publications

References 37 publications

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Prevalence, antibiotic resistance patterns, and biofilm formation ability of Enterobacterales recovered from food of animal origin in Egypt

Prevalence of Streptococci spp. and Unexpected Non-Streptococci Strains Associated with Bovine Mastitis Infection in Dairy Cattle in Region IV-A, Philippines

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