Characterization of cloned rat ribosomal DNA fragments

Fuke, Motohiro; Dennis, Kenrick J.; Busch, Harris

doi:10.1007/bf00422762

Cited by 28 publications

(11 citation statements)

References 21 publications

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“…Restriction maps for other rat rDNA clones (also from Sprague-Dawley libraries) have been published (4,27,32,33) and, in general, agree with our proposed map (Figure 1). One difference among the cloned rDNAs from different laboratories is the estimated size of the Eco RI fragment containing the initiation site.…”

Section: Genomic Organizationsupporting

confidence: 86%

Genomic organization of rat rDNA

et al. 1983

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A detailed restriction map was determined for a 10.9 KB region that contains the initiation site for 45S pre-rRNA and the first 1.7 KB of the 18S rRNA coding region. When the restriction pattern of the cloned rDNA was compared with that of total rat DNA, the rDNA regions of both Sprague-Dawley and BD-9 rats were identical to each other and to that of the cloned rDNA. However, both strains exhibit a major polymorphism consisting of an insertion of 0.9 KB of DNA in the nontranscribed spacer between 0.29 KB and 1.8 KB upstream from the 45S RNA initiation site. This region consists of tandem repeats approximately 130 base pairs in length. These repeats contain large poly T tracts and are similar in sequence to analogous elements 5' to the origin of mouse rRNA transcription. Regions containing highly repetitious DNA sequences were located at sites 2.8 KB and 4.3 KB upstream from the initiation site. The repetitive sequence at 2.8 KB from the initiation site anneal to a known Alu-equivalent type 2 sequence derived from the second intron of the rat growth hormone gene.

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Section: Genomic Organizationsupporting

confidence: 86%

Genomic organization of rat rDNA

et al. 1983

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“…The characterization of rat rDNA cloned in lambda bacteriophages and the recloning of the rDNA in plasmid pBR322 were previously described (5).…”

Section: Methodsmentioning

confidence: 99%

The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes

Torczynski¹,

Bollon²,

Fuke³

1983

Nucl Acids Res

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134

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The complete nucleotide sequence of the rat 18S ribosomal RNA gene has been determined. A comparison of the rat 18S ribosomal RNA gene sequence with the known sequences of yeast and frog revealed three conserved (stable) regions, two unstable regions, and three large inserts. (A,T) leads to (G,C) changes were more frequent than (G,C) leads to (A,T) changes for three comparisons (yeast leads to frog, frog leads to rat, and yeast leads to rat). GC pairs were inserted preferentially over AT pairs for the same three comparisons. These two factors contribute to the progressively higher GC content of 18S ribosomal RNA of yeast, frog, and rat.

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“…Drs. Richard Torczynski and Harris Busch (Wadley Institutes of Molecular Medicine, Dallas, TX) generously provided the rat ribosomal 18s RNA probe; the probe (pDF8) contained 0.9 kb of the middle portion of the 18s DNA (Torczynski et al, 1983) subcloned into pBR322 (Fuke et al, 1981). The v-ruPu cDNA probe was obtained from the American Type Culture Collection (ATCC No.…”

Section: Hybridization Probesmentioning

confidence: 99%

Developmental expression of cathepsin L and c‐ras^Ha in the mouse placenta

Hamilton

Bruns

Delgado

et al. 1991

Molecular Reproduction Devel

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An investigation is described of the expression of the cysteine proteinase cathepsin L during placental development. In addition, whether cathepsin L expression is linked to c-rasHa expression in development, as it is in metastatic cells, is examined. Large amounts of cathepsin L and its transcript are present in the mouse placenta, more than six times more than in adult kidney and liver. Throughout gestation, cathepsin L and its transcript are located in the giant cells and spongiotrophoblasts of the placenta. Several forms of different mobility on denaturing gels are found in the placenta. Their apparent molecular weights, as determined from the gels, are 43,000, 39,000, 29,000, and 20,000. The 39-kDa form is procathepsin L. The 29-kDa and 20-kDa forms are lysosomal cathepsin Ls. The 39-kDa procathepsin L and the 20-kDa mature cathepsin L are the most abundant species in the placenta and are present in about equal amounts throughout gestation. At any time during gestation, placental minces synthesize and secrete only procathepsin L. The amniotic fluid of the fetus contains the 43-kDa form of cathepsin L and procathepsin L, but no detectable amounts of mature cathepsin L. By contrast, serum from nonpregnant or pregnant mice contains three forms of cathepsin L (i.e., the 43-kDa form, procathepsin L, and mature cathepsin L). Cathepsin L and the rasHa oncogene are expressed in two coincident waves corresponding to periods during which the placenta is invasive and just before parturition. The presence of large amounts of cathepsin L in the placenta suggests that the proteinase has a significant function there. Expression of cathepsin L in the placenta is potentially under the control of the ras gene product p21; both are under developmental control.

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Characterization of cloned rat ribosomal DNA fragments

Cited by 28 publications

References 21 publications

Genomic organization of rat rDNA

Genomic organization of rat rDNA

The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes

Developmental expression of cathepsin L and c‐ras^Ha in the mouse placenta

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Characterization of cloned rat ribosomal DNA fragments

Cited by 28 publications

References 21 publications

Genomic organization of rat rDNA

Genomic organization of rat rDNA

The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes

Developmental expression of cathepsin L and c‐rasHa in the mouse placenta

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Product

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Developmental expression of cathepsin L and c‐ras^Ha in the mouse placenta