Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution.

Petryniak, Bronislawa; Staudt, Louis M.; Postema, Christina E.; McCormack, Wayne T.; Thompson, Craig B.

doi:10.1073/pnas.87.3.1099

Cited by 35 publications

(24 citation statements)

References 30 publications

(41 reference statements)

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“…The primary structure of the Oct-1 homeo domain is conserved among Xenopus (Smith and Old 1990), chickens (Petryniak et al 1990), and humans )--members of three separate vertebrate classes. During mammalian evolution, however, the homeo domain has varied.…”

Section: Implications Of Oct-1 Homeo Domain Variability In Mice and Hmentioning

confidence: 99%

Differential positive control by Oct-1 and Oct-2: activation of a transcriptionally silent motif through Oct-1 and VP16 corecruitment.

Cleary

Stern²,

Tanaka³

et al. 1993

Genes Dev.

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Transcriptional regulation by the ubiquitous human POU homeo domain protein Oct-1 and the related B-cell protein Oct-2 is a model for understanding how proteins that recognize the same regulatory site elicit different programs of gene transcription. Here, we describe a mechanism for differential promoter activation whereby only Oct-l, through selective corecruitment with the herpesvirus trans-activator VPI6, acquires the ability to stimulate transcription from a TAATGARAT-containing site that responds to neither Oct-1 nor Oct-2 alone. To measure differential in vivo activation by human Oct-1 and Oct-2 in response to VP16, we have developed a transient assay in murine NIH-3T3 cells. Surprisingly, murine Oct-1 associates with VP16 much less effectively than its human counterpart, most likely because the murine Oct-1 homeo domain differs at four positions from the human Oct-1 homeo domain. The murine cell transient assay shows directly that human Oct-l, but not human Oct-2, can respond to VP16 in vivo. The Oct-1 DNA-binding POU domain is sufficient and the Oct-1 homeo domain is critical for this response, because an Oct-1 POU domain containing the Oct-2 homeo domain fails to respond to the VP16-induced positive control of transcription. Thus, by selective homeo domain interaction and corecruitment to an otherwise silent regulatory element, VP16 expands the repertoire of sites responsive to Oct-1 without affecting the activity of its close relative Oct-2.

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Section: Implications Of Oct-1 Homeo Domain Variability In Mice and Hmentioning

confidence: 99%

Differential positive control by Oct-1 and Oct-2: activation of a transcriptionally silent motif through Oct-1 and VP16 corecruitment.

Cleary

Stern²,

Tanaka³

et al. 1993

Genes Dev.

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“…So far, five different classes can be distinguished, based on sequence homology (He et al 1989;Scholer et al 1990). They are present in murine tissue at different stages of development (Lenardo et al 1989;Scholer et al 1989a,b;Okamoto et al 1990), in adult human and rat brain (He et al 1989), chicken (Petryniak et al 1990), Xenopus laevis (Smith and Old 1990), C. elegans (Burglin et al 1989), and Diosophila (Johnson and Hirsh 1990), where they may function as neurospecific transcription factors.…”

mentioning

confidence: 99%

The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain.

Verrijzer¹,

Kal²,

Vliet³

1990

Genes Dev.

134

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The ubiquitous octamer-binding protein oct-1 contains a POU domain required for DNA binding, which can be subdivided into a POU-specific domain and a POU homeo domain. We have overproduced the POU domain and the POU homeo domain in a vaccinia expression system, purified both polypeptides to near homogeneity, and compared their DNA-binding properties. In contrast to the POU domain, the homeo domain protects only part of the octamer sequence in the Ad2 origin against breakdown by DNase I or hydroxyl radicals. Analysis of purine contacts by DMS and DEPC interference assays shows that the Ad2 octamer can be divided into two regions: one that is recognized both by the POU domain and the homeo domain in an identical fashion, and one that is only recognized by the POU domain. This suggests that the POU-specific domain is responsible for the additional contacts located at one side of the octamer. In agreement with this, mutating the first 3 nucleotides (ATG) of the octamer affected binding by the POU domain but not by the homeo domain. The apparent binding affinities to different octamer sites were compared. The homeo domain binds 600-fold less efficiently to the canonical octamer sequence (ATGCAAAT) than the POU domain. The difference is only sevenfold for the Ad2 octamer, whereas both K^ values are almost identical for the HSV ICP4 TAATGARAT motif. Both the POU and homeo domains recognize target sequences for mammalian homeo box proteins. We conclude that the octamer can act as a bipartite recognition sequence for oct-1 and that the POU-specific domain contributes to the binding affinity, as well as to the specificity, by providing additional contacts.

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“…Rapid isolation of Agt1O inserts by the polymerase chain reaction for 32p labeling by random priming was performed as described (25). Isolation of genomic clones and additional cDNA clones was performed using end-labeled oligonucleotides or the insert from cDNA 1340 radiolabeled by random priming (25,26 (25,27). The UG9 cell line was a gift of L. Schierman (University of Georgia).…”

mentioning

confidence: 99%

“…The UG9 cell line was a gift of L. Schierman (University of Georgia). The MSB-1 and UG9 cell lines were grown and passaged as described (16,26).…”

mentioning

confidence: 99%

Evolutionary conservation of antigen recognition: the chicken T-cell receptor beta chain.

Tjoelker

Carlson

Lee

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

106

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T cells play important regulatory roles in the immune responses of vertebrates. Antigen-specific T-cell activation involves T-cell receptor (TCR) recognition of a peptide antigen presented by a major histocompatibility complex molecule, and much has been learned about this antigenrecognition process through structural and genetic studies of mammalian TCRs. Although previous studies have demonstrated that avian T cells express cell-surface molecules analogous to the mammalian TCR heterodimers, TCR genes have not been identified in nonmammalian species. We now report the cloning of a cDNA that encodes the .8 chain of the chicken TCR. Southern blot analysis using this TCRfi cDNA probe demonstrated that the chicken TCR38 locus was clonally rearranged in chicken T-cell lines. TCRfi mRNA was expressed in cells isolated from the thymus but not in cells from the bursa of Fabricius where B cells are generated. Sequence analysis of six additional TCRfi cDNAs suggested the existence of at least two variable (V) region families, three joining (J) elements, and single diversity (D) and constant (C) elements. As in mammals, considerable nucleotide diversity was observed at the junctions of the variable, diversity, and joining elements in chicken TCRfi cDNAs. Genomic Vfi and Jfi elements were also cloned and sequenced. Both elements are flanked by classical heptamer/nonamer recombination signal sequences. Although the chicken and mammalian TCR.8 chains displayed only 31% overall amino acid sequence identity, a number of conserved structural features were observed. These data indicate that (t0 the chicken TCR, repertoire is generated by combinatorial and junctional diversity and (it) despite divergent evolution at the level of nucleotide sequence, important structural features of the TCRfi polypeptide are conserved between avian and mammalian species.

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Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution.

Cited by 35 publications

References 30 publications

Differential positive control by Oct-1 and Oct-2: activation of a transcriptionally silent motif through Oct-1 and VP16 corecruitment.

Differential positive control by Oct-1 and Oct-2: activation of a transcriptionally silent motif through Oct-1 and VP16 corecruitment.

The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain.

Evolutionary conservation of antigen recognition: the chicken T-cell receptor beta chain.

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