Characterization of centromere alterations in liposarcomas

A new multicolor-FISH approach for the characterization of marker chromosomes: centromere-specific multicolor-FISH (cenM-FISH)

“…Tumorcytogenetic markers with the involvement of heterochromatic material in HSR have not been frequently described (Liehr et al 1997;Sirvent et al 2000). By the application of cenM-FISH, juxtacentromeric material derived from chromosome 1 has been shown here to be amplified in a non-Hodgkin lymphoma case.…”

A new multicolor-FISH approach for the characterization of marker chromosomes: centromere-specific multicolor-FISH (cenM-FISH)

“…Tumorcytogenetic markers with the involvement of heterochromatic material in HSR have not been frequently described (Liehr et al 1997;Sirvent et al 2000). By the application of cenM-FISH, juxtacentromeric material derived from chromosome 1 has been shown here to be amplified in a non-Hodgkin lymphoma case.…”

HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX

“…Our results suggest that this imbalance can result not just in ectopic assembly of CENP-A, but also in modest recruitment of other kinetochore components, which correlate with increased mitotic defects [ 46 ], and amplification of the underlying DNA. This latter phenomenon is widely observed in human tumors [ 33 , 34 ]. Consistent with this idea, we present evidence that increasing CENP-A mislocalization and ectopic kinetochore formation may be one factor involved in the amplification and overexpression of MYC .…”

Neocentromeres: New Insights into Centromere Structure, Disease Development, and Karyotype Evolution

“…These cancers are marked by a supernumerary ring chromosome or large marker chromosome devoid of alpha-satellite DNA, comprised primarily of amplified 12q14-15 sequences that contain oncogenes 19,20 and possessing a neocentromere at the primary constriction. 21,22 This is in direct contrast to other liposarcomas, which contain the same amplified 12q14-15 sequences but on chromosomes with alphoid centromeres. 21 Thus, the presence of a marker chromosome with a neocentromere appears to be a defining characteristic of this tumor class.…”

“…21,22 This is in direct contrast to other liposarcomas, which contain the same amplified 12q14-15 sequences but on chromosomes with alphoid centromeres. 21 Thus, the presence of a marker chromosome with a neocentromere appears to be a defining characteristic of this tumor class.…”

A new multicolor-FISH approach for the characterization of marker chromosomes: centromere-specific multicolor-FISH (cenM-FISH)

“…Tumorcytogenetic markers with the involvement of heterochromatic material in HSR have not been frequently described (Liehr et al 1997;Sirvent et al 2000). By the application of cenM-FISH, juxtacentromeric material derived from chromosome 1 has been shown here to be amplified in a non-Hodgkin lymphoma case.…”

HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX

“…Our results suggest that this imbalance can result not just in ectopic assembly of CENP-A, but also in modest recruitment of other kinetochore components, which correlate with increased mitotic defects [ 46 ], and amplification of the underlying DNA. This latter phenomenon is widely observed in human tumors [ 33 , 34 ]. Consistent with this idea, we present evidence that increasing CENP-A mislocalization and ectopic kinetochore formation may be one factor involved in the amplification and overexpression of MYC .…”

Neocentromeres: New Insights into Centromere Structure, Disease Development, and Karyotype Evolution

“…These cancers are marked by a supernumerary ring chromosome or large marker chromosome devoid of alpha-satellite DNA, comprised primarily of amplified 12q14-15 sequences that contain oncogenes 19,20 and possessing a neocentromere at the primary constriction. 21,22 This is in direct contrast to other liposarcomas, which contain the same amplified 12q14-15 sequences but on chromosomes with alphoid centromeres. 21 Thus, the presence of a marker chromosome with a neocentromere appears to be a defining characteristic of this tumor class.…”

“…21,22 This is in direct contrast to other liposarcomas, which contain the same amplified 12q14-15 sequences but on chromosomes with alphoid centromeres. 21 Thus, the presence of a marker chromosome with a neocentromere appears to be a defining characteristic of this tumor class.…”

A new multicolor-FISH approach for the characterization of marker chromosomes: centromere-specific multicolor-FISH (cenM-FISH)

“…Tumorcytogenetic markers with the involvement of heterochromatic material in HSR have not been frequently described (Liehr et al 1997;Sirvent et al 2000). By the application of cenM-FISH, juxtacentromeric material derived from chromosome 1 has been shown here to be amplified in a non-Hodgkin lymphoma case.…”

HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX

“…Our results suggest that this imbalance can result not just in ectopic assembly of CENP-A, but also in modest recruitment of other kinetochore components, which correlate with increased mitotic defects [ 46 ], and amplification of the underlying DNA. This latter phenomenon is widely observed in human tumors [ 33 , 34 ]. Consistent with this idea, we present evidence that increasing CENP-A mislocalization and ectopic kinetochore formation may be one factor involved in the amplification and overexpression of MYC .…”

Neocentromeres: New Insights into Centromere Structure, Disease Development, and Karyotype Evolution

“…These cancers are marked by a supernumerary ring chromosome or large marker chromosome devoid of alpha-satellite DNA, comprised primarily of amplified 12q14-15 sequences that contain oncogenes 19,20 and possessing a neocentromere at the primary constriction. 21,22 This is in direct contrast to other liposarcomas, which contain the same amplified 12q14-15 sequences but on chromosomes with alphoid centromeres. 21 Thus, the presence of a marker chromosome with a neocentromere appears to be a defining characteristic of this tumor class.…”

“…21,22 This is in direct contrast to other liposarcomas, which contain the same amplified 12q14-15 sequences but on chromosomes with alphoid centromeres. 21 Thus, the presence of a marker chromosome with a neocentromere appears to be a defining characteristic of this tumor class.…”