Characterization of CD1e, a Third Type of CD1 Molecule Expressed in Dendritic Cells

Angénieux, Catherine; Salamero, Jean; Fricker, Dominique; Cazenave, Jean‐Pierre; Goud, Bruno; Hanau, Daniel

doi:10.1074/jbc.m007082200

Cited by 81 publications

(100 citation statements)

References 25 publications

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“…S4) and partially colocalized with the Golgi marker TGN-38 and with MHC class II compartment (Fig. S3B), thus showing a distribution similar to the one occurring in human cells (1). Number and phenotype of iNKT cells in thymus and periphery as well as of other lymphocyte populations and monocytes were comparable in Tg and WT mice (Figs.…”

Section: Facilitated Presentation Of Lipid Antigens In Cd1e Transgenimentioning

confidence: 71%

“…CD1e is the only CD1 molecule that becomes soluble and is never expressed on the plasma membrane, thus it cannot behave as an antigen-presenting molecule. Moreover, CD1e shows a very peculiar intracellular distribution, which in dendritic cells (DCs) correlates with their maturation stage (1). In immature DCs, CD1e prevalently accumulates in the trans-Golgi network (TGN), whereas, in mature DCs, it distributes in late endosomes and lysosomes (LYs) where it is cleaved into an active soluble form (2).…”

Section: Cd1 Restriction | Lipid Transfer Proteins | Natural Killer Tmentioning

confidence: 99%

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Fine tuning by human CD1e of lipid-specific immune responses

Facciotti

Cavallari

Angénieux

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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CD1e is a member of the CD1 family that participates in lipid antigen presentation without interacting with the T-cell receptor. It binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. We find that CD1e may positively or negatively affect lipid presentation by CD1b, CD1c, and CD1d. This effect is caused by the capacity of CD1e to facilitate rapid formation of CD1-lipid complexes, as shown for CD1d, and also to accelerate their turnover. Similar results were obtained with antigen-presenting cells from CD1e transgenic mice in which lipid complexes are assembled more efficiently and show faster turnover than in WT antigen-presenting cells. These effects maximize and temporally narrow CD1-restricted responses, as shown by reactivity to Sphingomonas paucimobilis-derived lipid antigens. CD1e is therefore an important modulator of both group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability as well as the generation and persistence of CD1-lipid complexes.CD1 restriction | lipid transfer proteins | natural killer T cells

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Section: Facilitated Presentation Of Lipid Antigens In Cd1e Transgenimentioning

confidence: 71%

Section: Cd1 Restriction | Lipid Transfer Proteins | Natural Killer Tmentioning

confidence: 99%

Fine tuning by human CD1e of lipid-specific immune responses

Facciotti

Cavallari

Angénieux

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…group 3 CD1). It appears to be expressed mainly intracellularly or as a secreted protein, and its potential function is currently unknown (10).…”

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confidence: 99%

Direct Measurement of Antigen Binding Properties of CD1 Proteins Using Fluorescent Lipid Probes

Illarionov

et al. 2004

Journal of Biological Chemistry

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Section: Introductionmentioning

confidence: 99%

“…In contrast, CD1e is not expressed on the cell surface and is an intermediate form in terms of its homology to the other CD1 molecules. Accordingly, it is regarded as a group 3 CD1 molecule [3].By trafficking through endosomal compartments, CD1 proteins pick up antigens in a similar fashion to MHC class II molecules [4]. However, instead of peptides, CD1 molecules present lipids, glycolipids or lipopeptides of either foreign or self origin [2,5].…”

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confidence: 99%

Viral danger signals control CD1d de novo synthesis and NKT cell activation

et al. 2008

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The nonpolymorphic CD1 molecules present lipid antigens to T cells. In myeloid DC humans express five different CD1 proteins (CD1a-e; the corresponding CD1 genes are designated CD1A-E). A role for CD1d-restricted NKT cells in the control of virus infections has been delineated from clinical observations, mouse models and viral evasion mechanisms targeting CD1d. How NKT cells are activated by virus infections is unclear. We found that human myeloid DC differentially regulate CD1 antigen presentation in response to viral danger signals. Stimulation with type I IFN, viral TLR ligands or viruses strongly enhanced the number of CD1D transcripts in human myeloid DC but diminished the abundance of CD1A, CD1B and CD1E mRNA. These changes on the transcriptional level were mirrored by altered cellular distribution and increased surface expression of CD1d. As a consequence NKT cells were activated and showed a Th1-like response. Moreover, NKT cell activation in PBMC exposed to viral danger signals was dependent on human plasmacytoid DC which produce large amounts of IFN-a. In conclusion, our data indicate that viral danger signals trigger NKT cell activation by enhancing CD1d de novo synthesis through increasing the abundance of CD1D mRNA in human myeloid DC. IntroductionThe immune system detects viruses by recognizing antigens loaded on specialized surface molecules. The highly polymorphic MHC molecules bind to diverse peptides, traffic to the cell surface, and display this information to T cells [1]. Although distantly related to MHC proteins, the nonpolymorphic CD1 proteins constitute an evolutionarily distinct family of antigenpresenting molecules [2]. Humans express five different CD1 proteins (CD1a-e; the corresponding CD1 genes are designated CD1A-E). They are further divided into group 1 (CD1a-c) and group 2, which contains CD1d as the sole member. In contrast, CD1e is not expressed on the cell surface and is an intermediate form in terms of its homology to the other CD1 molecules. Accordingly, it is regarded as a group 3 CD1 molecule [3].By trafficking through endosomal compartments, CD1 proteins pick up antigens in a similar fashion to MHC class II molecules [4]. However, instead of peptides, CD1 molecules present lipids, glycolipids or lipopeptides of either foreign or self origin [2,5]. The CD1d-restricted NKT cells can also recognize a-galactosylceramide (aGalCer), a well-studied and highly potent CD1d ligand derived from a marine sponge [6,7]. These [18,25] or improves disease outcome [12,26]. Moreover, viruses subvert antigen presentation through CD1d [27][28][29][30][31][32][33], indicating its importance in antiviral immune defense.The mechanisms leading to activation of CD1d-restricted NKT cells in humans infected with viral pathogens are unknown. However, DC will play an important role as they link the innate and adaptive antiviral immunity [34]. DC sense invading viruses by detecting conserved pathogen-associated molecular patterns through a variety of different pattern recognition receptors, e.g. TLR...

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Characterization of CD1e, a Third Type of CD1 Molecule Expressed in Dendritic Cells

Cited by 81 publications

References 25 publications

Fine tuning by human CD1e of lipid-specific immune responses

Fine tuning by human CD1e of lipid-specific immune responses

Direct Measurement of Antigen Binding Properties of CD1 Proteins Using Fluorescent Lipid Probes

Viral danger signals control CD1d de novo synthesis and NKT cell activation

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