Characterization of C-type natriuretic peptide receptors in human mesangial cells

Zhao, Jun; Ardaillou, Nicole; Lu, Changyong; Placier, Sandrine; Pham, Paul; Lakhdar, B.; Cambar, J.; Ardaillou, Raymond

doi:10.1038/ki.1994.326

Cited by 35 publications

(20 citation statements)

References 23 publications

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“…The existence of the NPR-A receptor was also suggested earlier by electrophysiological observations from these cells [34]. Using immunohistochemistry and PCR analysis it was suggested that the dominantly expressed natriuretic peptide receptor in human MCs is NPR-B [35,36]. These findings indicate marked species differences in the expression of receptors for natriuretic peptides.…”

Section: Discussionmentioning

confidence: 59%

Natriuretic Peptides and Diadenosine Polyphosphates Modulate pH Regulation of Rat Mesangial Cells

Schulte

Hohendahl

Stegemann

et al. 1999

Cell Physiol Biochem

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Modulation of cell proliferation has often been thought to be connected to changes in the activity of pH-regulatory transporters and consequently intracellular pH (pH_i). The influence of natriuretic peptides, diadenosine polyphosphates, adenosine and ATP as well as platelet-derived growth factor (PDGF) on pH_i regulation of cultured rat mesangial cells was examined with the pH-sensitive dye 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. The inhibitors of Na⁺/H⁺ exchange, amiloride and HOE694, blocked pH_i recovery completely in the absence of and by approximately 50% in the presence of HCO₃^–/CO₂. Natriuretic peptides (ANP, BNP, CNP, urodilatin) completely inhibited pH_i recovery in the absence of and by approximately 40% in the presence of HCO₃^–/CO₂. These effects were abolished by the cGMP-dependent protein kinase inhibitor KT5823. Diadenosine polyphosphates (Ap3A-Ap6A), ATP and adenosine also inhibited pH_i recovery completely in the absence of and partially (30–40%) in the presence of HCO₃^–/ CO₂. The effect of adenosine was abolished in the presence of the cAMP-dependent protein kinase inhibitor KT5720, and that of Ap5A by the protein kinase C inhibitor calphostin C. PDGF activated acid extrusion in these cells by approximately 40%. From the four cloned isoforms of the Na⁺/H⁺ exchanger in the rat, only transcripts of NHE-1 were found in these mesangial cell cultures using RT-PCR analysis. These data suggest that in these rat mesangial cells the Na⁺/H⁺ exchanger, specifically the NHE-1 isoform, accounts for around 50% of pH_i recovery from an acid load under physiological conditions, and that Na⁺/H⁺ exchange stimulated by acidification can be inhibited by activation of PKG, PKA, and PKC and stimulated by PDGF after acute exposition to these agonists.

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Section: Discussionmentioning

confidence: 59%

Natriuretic Peptides and Diadenosine Polyphosphates Modulate pH Regulation of Rat Mesangial Cells

Schulte

Hohendahl

Stegemann

et al. 1999

Cell Physiol Biochem

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“…At the end of the incubation period, the supernatant was discarded. Intracellular cyclic AMP was extracted with an ethanol:formic acid mixture (95:5; v/v) and measured by radioimmunoassay as previously described [19]. …”

Section: Methodsmentioning

confidence: 99%

Characterization of B<sub>2</sub>-Bradykinin Receptors in Rabbit Principal Cells of the Collecting Duct

Ardaillou¹,

Placier²,

Zhao³

et al. 1998

Nephron Exp Nephrol

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Bradykinin (BK) plays a key role in collecting duct functions. Using an established line of principal cells of the rabbit collecting duct (R.C. SV3), we examined the characteristics of the BK receptors in these cells. [³H]-BK bound specifically to R.C. SV3. Saturation binding analyses allowed K_D (968 ± 232 pM) and B_max values (356 ± 43 fmol/mg protein) to be calculated. Competitive displacement of [³H]-BK was observed with Hoe-140, a specific type 2 BK receptor (BKR-2) antagonist, but not with des arg⁹-BK, a BKR-1 agonist. The presence of BKR-2 was confirmed by the reverse-transcription polymerase chain reaction technique. BK stimulated cytosolic calcium and inositol phosphate formation in a dose-dependent manner (from 1 nM to 1 µM). BK also inhibited the arginine vasopressin dependent increase of cyclic adenosine monophosphate. This effect could not be related to the production of prostaglandin E₂. These results demonstrate the presence of high-affinity BKR-2 in the principal cells of the rabbit collecting duct that are linked to phospholipase C activity and are involved in arginine vasopressin related regulatory loops.

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“…ANP-induced cGMP formation was regulated by protein kinase A (PKA), since H-89, a PKA inhibitor, decreased ANP-stimulated cGMP formation in podocytes (248). ANP, BNP, and CNP stimulated concentration-dependent cGMP formation, and in RT-PCR studies, mRNA for NPR-A, -B, and -C receptors was detected in human podocytes (514). The functional consequences of cGMP increase on podocytes are poorly understood.…”

Section: A Cgmp Signalingmentioning

confidence: 99%

Cell Biology of the Glomerular Podocyte

Pavenstädt

Kriz

Kretzler

2003

Physiological Reviews

1,308

1,278

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Glomerular podocytes are highly specialized cells with a complex cytoarchitecture. Their most prominent features are interdigitated foot processes with filtration slits in between. These are bridged by the slit diaphragm, which plays a major role in establishing the selective permeability of the glomerular filtration barrier. Injury to podocytes leads to proteinuria, a hallmark of most glomerular diseases. New technical approaches have led to a considerable increase in our understanding of podocyte biology including protein inventory, composition and arrangement of the cytoskeleton, receptor equipment, and signaling pathways involved in the control of ultrafiltration. Moreover, disturbances of podocyte architecture resulting in the retraction of foot processes and proteinuria appear to be a common theme in the progression of acquired glomerular disease. In hereditary nephrotic syndromes identified over the last 2 years, all mutated gene products were localized in podocytes. This review integrates our recent physiological and molecular understanding of the role of podocytes during the maintenance and failure of the glomerular filtration barrier.

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Characterization of C-type natriuretic peptide receptors in human mesangial cells

Cited by 35 publications

References 23 publications

Natriuretic Peptides and Diadenosine Polyphosphates Modulate pH Regulation of Rat Mesangial Cells

Natriuretic Peptides and Diadenosine Polyphosphates Modulate pH Regulation of Rat Mesangial Cells

Characterization of B<sub>2</sub>-Bradykinin Receptors in Rabbit Principal Cells of the Collecting Duct

Cell Biology of the Glomerular Podocyte

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