Characterization of biofilm formation by Riemerella anatipestifer

Hu, Qinghai; Han, Xiangan; Zhou, Xiaojin; Ding, Siyu; Ding, Chan; Yu, Shuang

doi:10.1016/j.vetmic.2010.02.023

Cited by 60 publications

(44 citation statements)

References 18 publications

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“…Thirty-three RA strains were isolated from infected duck farms in China between 1997 and 2009 [15], including 15 strains of serotype 1 (WJ4, CH3, NJ1, NJ2, NJ4, CQ1, CQ3, CQ4, CQ5, JY4, YL4, YXB12, YXB14, NN2 and NN3), ten strains of serotype 2 (SC2, NJ3, B2, TH4, YXB1, NN5, GD3, GD4, GD5 and GD7), three strains of serotype 10 (YXL1, HXB2 and YXB11), one strain of serotype 15 (NN6) and four strains of unknown serotype (NN11, GD1, GD6 and JY6). All the RA strains were cultured in tryptic soy broth (TSB, Difco, Franklin Lakes, NJ, USA) at 37 C with 5% CO 2 .…”

Section: Bacterial Strains Plasmids and Culture Conditionsmentioning

confidence: 99%

Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation

Han

Liu

Fan

et al. 2015

Research in Microbiology

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Section: Bacterial Strains Plasmids and Culture Conditionsmentioning

confidence: 99%

Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation

Han

Liu

Fan

et al. 2015

Research in Microbiology

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“…Strains YL4 (serotype 1), Yb2 (serotype 2), and HXb2 (serotype 10) were used as the challenge strains based on our previous studies (14,15,16). All of the strains were isolated in China (17) and cultured in tryptic soy agar (TSA) (Difco, Franklin Lakes, NJ) at 37°C for 24 h in 5% CO 2 or tryptic soy broth (TSB) (Difco) at 37°C and 150 rpm for 8 to 12 h.…”

Section: Animalsmentioning

confidence: 99%

Development and Evaluation of a Trivalent Riemerella anatipestifer-Inactivated Vaccine

Hai-wen¹,

Wang²,

Ding³

et al. 2013

Clin. Vaccine Immunol.

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b Riemerella anatipestifer infections cause major economic losses in the duck industry. In this study, a trivalent inactivated vaccine of R. anatipestifer, including strains CH3 (serotype 1), NJ3 (serotype 2), and HXb2 (serotype 10), was developed. Animal experiments showed that the ducks that received two immunizations with the vaccine were 100% protected from challenge with strains from any of the three serotypes (1, 2, or 10). No death or clinical signs of diarrhea, tremors, or limb swelling were shown in the protected ducks. Also, no R. anatipestifer bacteria were isolated from the livers or brains of the protected ducks. Furthermore, no histopathological changes were observed in the liver, spleen, or brain samples from the protected ducks during histological examination. The ducks that received two immunizations with the vaccine generated high antibody titers of 1:3,200 to 1:6,400 against the three serotypes of strains. The vaccine significantly enhanced the production of gamma interferon (IFN-␥) and interleukin 2 (IL-2) after one immunization and enhanced the production of IL-4 and IL-10 after two immunizations. In addition, real-time PCR indicated that the expression of major histocompatibility complex I (MHC-I), as well as that of CD40 and CD154 molecules, was significantly increased after one immunization, and the expressions of both MHC-I and MHC-II molecules were increased after two immunizations. Our study indicates that the vaccine can induce both humoral and cellular immunities in ducks and offer effective protection against R. anatipestifer infection.

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“…Biofilm formation by diverse bacteria is considered an ecological niche and a protection against antimicrobial agents as of the immune response of host (Stewart & Costerton, 2001;Hu et al, 2010;Chen et al, 2012). In this research, we were able to confirm the capacity of ORT to form biofilm, as occurred with S. mutans, which was the strain used as the positive control for biofilm formation.…”

Section: Discussionmentioning

confidence: 57%

“…All experiments were performed in triplicate, and the mean-one standard deviation for each isolate was calculated from three independent experiments. The BHI medium and S. mutans ATCC 25125 were used as negative and positive controls for biofilm formation (Carrillo et al, 2010;Hu et al, 2010;Chen et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Conditions that induce biofilm production byOrnithobacterium rhinotracheale

Rosa-Ramos¹,

Rodríguez-Cruz

López-Villegas

et al. 2015

Avian Pathology

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Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO 2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO 2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.

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Characterization of biofilm formation by Riemerella anatipestifer

Cited by 60 publications

References 18 publications

Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation

Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation

Development and Evaluation of a Trivalent Riemerella anatipestifer-Inactivated Vaccine

Conditions that induce biofilm production byOrnithobacterium rhinotracheale

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