Characterization of Beauveria bassiana1 Isolates Associated with Euwallacea sp. nr. fornicatus2 in Populus sp.

Montesinos-Matías, Roberto; Gallou, Adrien; Berlanga-Padilla, Angélica María; Serna-Domínguez, María G.; Laureano-Ahuelicán, Bruno; Ayala-Zermeño, Miguel Angel; Ordáz-Hernández, A.; López-Buenfil, José Abel; Arredondo-Bernal, Hugo C.

doi:10.3958/059.044.0207

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Vuill. (Hypocreales: Clavicipitaceae), is a fungus species isolated from borer beetles (Selvasundaram & Muraleedharan, 2000;Wegensteiner, Wermelinger & Herrmann, 2015;Montesinos-Matías et al, 2019a). Popa et al (2012) confirmed that B. bassiana was reported infecting 12 borer beetle species.…”

Section: Introductionmentioning

confidence: 94%

Selection ofBeauveria bassiana(Hypocreales: Cordycipitaceae) strains to controlXyleborus affinis(Curculionidae: Scolytinae) females

Castrejón-Antonio

Tamez‐Guerra

Montesinos-Matías

et al. 2020

PeerJ

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Background Xyleborus affinis Eichhoff (Coleoptera: Curculionidae) is an ambrosia beetle reported to affect avocado trees (Persea americana Mill.). The use of the entomopathogenic fungus (EPF) Beauveria bassiana (Bals.-Criv.) Vuill. for ambrosia beetle control represents an alternative to insecticides. Methods This study was designed in two stages to select B. bassiana strains with potential to control X. affinis females. In the first stage, 19 B. bassiana Mexican strains from EPF collection, isolated from Coleoptera (CHE-CNRCB, http://www.gob.mx/senasica/documentos/coleccion-de-hongos-entomopatogenos), were tested. Analyses included radial growth rate, conidial yield, spore germination, and germ tube length. Results were analysed by Principal Component Analysis (PCA) to identify clusters within favourable growth phenotypes. For the second stage, 10 selected strains were re-analysed for virulence-related metabolic characteristic, including cell wall-bound cuticle-degrading enzymes–Pr1-like proteases and β-N-acetyl glucosaminidases (NAGase) chitinases, conidial hydrophobicity and monopolar germination parameters. A second PCA analysis was run for those virulence parameters analysed, and upon results strains CHE-CNRCB 44, 171, 431 and 485 were selected and tested against X. affinis females. Females were treated with a 1 × 108 conidia mL−1 suspension (recommended rate), using a Potter Tower. Results All strains showed insecticidal activity, inducing up to 58% mortality; about 30% dead beetles developed aerial mycelia (CHE-CNRCB 485) and the fastest mortality rate was t0 = 1.95 (CHE-CNRCB 44). Conclusion Since all selected strains showed virulence against X. affinis females, results indicated the possibility of selecting B. bassiana strains based on multiple metabolic attributes, as a preliminary test to perform bioassays against order-related target insects.

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Section: Introductionmentioning

confidence: 94%

Selection ofBeauveria bassiana(Hypocreales: Cordycipitaceae) strains to controlXyleborus affinis(Curculionidae: Scolytinae) females

Castrejón-Antonio

Tamez‐Guerra

Montesinos-Matías

et al. 2020

PeerJ

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“…Beauveria bassiana is a promising fungus for the control of ambrosial beetles. Several strains of B. bassiana infect more than 12 species of bark beetles [ 2 , 19 , 20 , 21 ]. In this regard, it has been reported that the highest mortality and mycosis percentage against X. glabratus was caused by a B. bassiana -based commercial product called Botanigard ® ES, as compared with other fungal products tested, under laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Xyleborus affinis (Curculionidae: Scolitinae) Females and Progeny by Beauveria bassiana (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model

Castrejón-Antonio

Tamez‐Guerra

García-Ortiz

et al. 2023

Insects

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The ambrosia beetle Xyleborus affinis, recently reported affecting avocado trees in Mexico, represents one of the most widespread insects worldwide. Previous reports have shown that Xyleborus genera members are susceptible to Beauveria bassiana and other entomopathogenic fungus strains. However, their effect on borer beetles’ progeny has not been fully investigated. The aim of the present study was to determine the insecticidal activity of B. bassiana on X. affinis adult females and their progeny in an artificial sawdust diet bioassay model. The B. bassiana strains CHE-CNRCB 44, 171, 431, and 485 were individually tested on females at concentrations ranging from 2 × 106 to 1 × 109 conidia mL−1. After 10 d of incubation, diet was evaluated to count laid eggs, larvae, and adults. Insect conidia loss after exposure was determined by attached conidia to each insect after 12 h of exposure. The results showed that females’ mortality ranged between 3.4% and 50.3% in a concentration–response manner. Furthermore, we did not observe statistical differences among strains at the highest concentration. CHE-CNRCB 44 showed the highest mortality at the lowest concentration and reduced larvae and laid eggs at the highest concentration (p < 0.01). Strains CHE-CNRCB 44, 431, and 485 significantly decreased larvae, as compared with the untreated control. After 12 h, up to 70% of conidia was removed by the effect of the artificial diet. In conclusion, B. bassiana has the potential to control X. affinis adult females and progeny.

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Metarhizium robertsii in combination with Trichoderma asperellum reduce the malathion doses used to control ambrosia beetles: the case of Xyleborus affinis

Reynoso-López

Hernández

Ek-Ramos

et al. 2021

Biocontrol Science and Technology

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Characterization of Beauveria bassiana1 Isolates Associated with Euwallacea sp. nr. fornicatus2 in Populus sp.

Cited by 3 publications

References 13 publications

Selection ofBeauveria bassiana(Hypocreales: Cordycipitaceae) strains to controlXyleborus affinis(Curculionidae: Scolytinae) females

Selection ofBeauveria bassiana(Hypocreales: Cordycipitaceae) strains to controlXyleborus affinis(Curculionidae: Scolytinae) females

Biocontrol of Xyleborus affinis (Curculionidae: Scolitinae) Females and Progeny by Beauveria bassiana (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model

Metarhizium robertsii in combination with Trichoderma asperellum reduce the malathion doses used to control ambrosia beetles: the case of Xyleborus affinis

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