Characterization of bacteriophage <scp>pA</scp>h‐1 and its protective effects on experimental infection of <i>Aeromonas hydrophila</i> in Zebrafish (<i>Danio rerio</i>)

Easwaran, Maheswaran; Dananjaya, S.H.S.; Park, Taesung; Lee, J; Shin, Hyun‐Jin; Zoysa, Mahanama De

doi:10.1111/jfd.12536

Cited by 38 publications

(30 citation statements)

References 19 publications

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“…1d). Latent periods and burst sizes of reported Aeromonas phages differed over a wide range, considerably high burst size (626 PFU/infected cells) and short latent period (15 min) [19] to very low burst size (2 PFU/infected cells) and long latent periods (150 min) [17]. These two parameters in our study were compatible with reported A. hydrophila phages [18], and showed smaller burst size compared to BPA6 [16] and Φ2 [7].…”

Section: Resultssupporting

confidence: 88%

Isolation and characterization of phage AHP-1 and its combined effect with chloramphenicol to control Aeromonas hydrophila

et al. 2019

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To develop an alternative bio-control measure for multi-drug resistant pathogenic Aeromonas hydrophila, which causes motile Aeromonas septicemia in fish, novel virulent phage (AHP-1) was isolated from carp tissues. Morphological analysis by transmission electron microscopy revealed that AHP-1 belongs to Myoviridae family. AHP-1 displayed 81% of moderate adsorption by 25 min, and latent period of 40 min with burst size of 97 PFU mL −1 at an optimal multiplicity of infection (MOI) 0.1. AHP-1 was stable over a broad range of pH (4-11), temperature (4-50°C), and salinity (0.1-3.5%). Both time and MOI dependent in vitro A. hydrophila growth inhibition was observed with AHP-1. AHP-1 (10 MOI) showed higher growth inhibition against A. hydrophila than chloramphenicol (5 μg mL −1), and combined treatment was more promising than individuals. Immune gene expression analysis of zebrafish upon continuous bath exposure to AHP-1 resulted significantly higher (il-6 and sod-1) or slight induction (tnf-α, il1-β, il-10, and cxcl-8a) than controls at beginning of the phage exposure, but those lowered to basal level by day 12 post-phage exposure. It suggests no adverse immune responses have occurred for the AHP-1 dose that used, and have potential for the phage therapy. Further detailed in vivo studies are needed to confirm the protective efficacy of newly isolated AHP-1 against A. hydrophila infection.

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Section: Resultssupporting

confidence: 88%

Isolation and characterization of phage AHP-1 and its combined effect with chloramphenicol to control Aeromonas hydrophila

et al. 2019

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“…We showed interest in the AS‐R5 strain because phage SW69‐9 formed clear plaques compared to all those observed in the previous study which were turbid (Vincent et al , ). The observation of such phage plaque differences has been made also by others in A. salmonicida (Easwaran et al , ). By comparing the lytic phenotype of several strains with or without functional A‐layers, the role of the A‐layer in turbid plaque seems evident.…”

Section: Discussionsupporting

confidence: 55%

Beyond the A‐layer: adsorption of lipopolysaccharides and characterization of bacteriophage‐insensitive mutants of Aeromonas salmonicida subsp. salmonicida

Paquet

Vincent

Moineau

et al. 2019

Molecular Microbiology

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helped to identify mutations in genes involved in the biogenesis of lipopolysaccharides (LPS) and on an uncharacterized gene (ASA_1998 ). The characterization of the LPS profile and gene complementation assays identified LPS as a phage receptor and confirmed the involvement of the uncharacterized protein ASA_1998 in phage infection. In addition, we confirmed that the presence of an A-layer at the bacterial surface could act as protection against phages. This study brings new elements into our understanding of the phage adsorption to A. salmonicida subsp. salmonicida cells.

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“…Their therapeutic effects have been confirmed in animals, humans, crops, and aquaculture [7,8,10]. Several studies have reported the isolation of Aeromonas phages and examined their protective effects against disease [3,14,15].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported the isolation of Aeromonas phages and the investigation of their protective effects against Aeromonas salmonicida [26][27][28] and A. hydrophila [3,14]. Although the results of these studies suggested their protective effects against Aeromonas infection, the degrees of protection are different.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

et al. 2020

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Bacteriophages are increasingly being used as biological control agents against pathogenic bacteria. In the present study, we isolate and characterize bacteriophage Akh-2 from Geoje Island, South Korea, to evaluate its utility in controlling motile Aeromonas septicemia. Akh-2 lysed four of the seven Aeromonas hydrophila strains tested. Transmission electron microscopy analysis showed that Akh-2 belongs to the Siphoviridae family, with head and tail sizes of 50 ± 5 and 170 ± 5 nm, respectively. One-step growth curve analysis revealed that the phage has a latent period of 50 ± 5 min and a burst size of 139 ± 5 plaque-forming units per infected cell. The phage appeared stable in a pH range of 6–8 and a temperature range of −80 to 46 °C. Based on next-generation sequencing analysis, its genome is 114,901 bp in size, with a 44.22% G + C content and 254 open reading frames. During an artificial induction of the disease, loach (Misgurnus anguillicaudatus) treated with Akh-2 showed an increased survival rate and time compared with the non-treated control. Our results suggest that Akh-2 is a potential biological agent for the treatment of Aeromonas infections in fish.

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Characterization of bacteriophage pAh‐1 and its protective effects on experimental infection of Aeromonas hydrophila in Zebrafish (Danio rerio)

Cited by 38 publications

References 19 publications

Isolation and characterization of phage AHP-1 and its combined effect with chloramphenicol to control Aeromonas hydrophila

Isolation and characterization of phage AHP-1 and its combined effect with chloramphenicol to control Aeromonas hydrophila

Beyond the A‐layer: adsorption of lipopolysaccharides and characterization of bacteriophage‐insensitive mutants of Aeromonas salmonicida subsp. salmonicida

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

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