Characterization of avian pathogenic Escherichia coli isolated in eastern China

Dou, Xiaowei; Gong, Jicheng; Han, Xiangan; Xu, Ming; Shen, Haiyu; Zhang, Di; Zhuang, Liying; JiaSheng, Liu; Zou, Jianmin

doi:10.1016/j.gene.2015.10.012

Cited by 64 publications

(62 citation statements)

References 23 publications

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“…Of the 15 tested antimicrobials, E. coli were most resistant to cephalothin, nitrofurantoin, and ampicillin (Table ). These results are consistent with previous findings (Dou et al, ; Parveen et al, ; Rees et al, ; Ryu, Lee, et al, ). Authors have reported that E. coli isolates are generally resistant to antimicrobials which have been in use the longest time in human and veterinary medicine, like nitrofurantoin, ampicillin, and tetracycline (Dou et al, ).…”

Section: Discussionsupporting

confidence: 94%

“…Antimicrobial resistance (AMR) is another tool for characterization of bacterial isolates. With the wide use of antimicrobials in humans and in the environment, AMR E. coli have been reported from different sources and countries (Dou et al, 2016;Kao et al, 2016;Rabbia et al, 2016;Zhang et al, 2016). The presence of resistant organisms in the environment is an emerging concern around the world (Watkinson, Micalizzi, Graham, Bates, & Costanzo, 2007), and since AMR bacteria can accumulate in bivalve mollusks (Barkovskii, Green, & Hurley, 2010), it has been suggested that bivalves may be useful in assessing environmental contamination by AMR bacteria (Berendonk et al, 2015;Rees et al, 2015).…”

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confidence: 99%

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Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

et al. 2018

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The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty‐one E. coli isolates were characterized for serogroups with 181 specific O antisera and antimicrobial susceptibility using the disk diffusion method. The genetic diversity was assessed using pulsed‐field gel electrophoresis (PFGE). The results showed that among the isolates, 19.9% were classified as multi‐drug resistant (MDR) and resistance was most frequently observed to cephalothin, nitrofurantoin, and ampicillin. The predominant serogroups were O6, O8, and O38. Some serogroups were recognized as pathogenic E. coli . PFGE dendrograms indicated extensive genetic diversity among the isolates. Although characteristics of the E. coli isolates were highly variable, it is important to note that E. coli belonging to pathogenic serogroups and MDR isolates are present in mollusks of both study areas. This is the first report on the phenotypic and genotypic characterization of E. coli from mollusks from Santa Catarina and the Chesapeake Bay that should encourage studies focusing on comparison of isolates across countries.

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Section: Discussionsupporting

confidence: 94%

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confidence: 99%

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

et al. 2018

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“…Because China is a large country of duck production customers, duck breeding accounts for a significant part of Chinese agriculture. Nevertheless, with the development of industrial‐scale production and a significantly increased stocking density, contagious diseases of duck, especially APEC, H9 AIV, TMUV, and DPV, have gradually become more epidemic and severe (Dou et al., ; Lee et al., ; Li et al., ; Liu, Zhang, Chen, Wang, & Cheng, ; Sun et al., ; Wang et al., , ). All these infections induce a high mortality or severe losses of productivity in ducks, whereas coinfection among them further aggravates the diseases, making the conditions worse and leading to extensive damage to the duck industry.…”

Section: Discussionmentioning

confidence: 99%

Duck viral infection escalated the incidence of avian pathogenic Escherichia coli in China

Liu

Diao

Wang

et al. 2019

Transbound Emerg Dis

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Avian pathogenic Escherichia coli (APEC) causes high mortality in poultry flocks and often is complicated with viral infections, leading to large economic losses; however, little information is available on the epidemiological characteristics of this pathogen in ducks. Therefore, a systemic epidemiological investigation was performed on 325 duck farms from 13 provinces in China during the period of 1 April 2016 until 31 March 2018, covering 2 years. A total of 26 APEC strains were isolated from different farms in this study, and analysis showed that all of those isolates carried multiple virulence‐associated genes and drug‐resistance genes, which led to high pathogenicity (15/26), strong or moderate biofilm formation (24/26) and multidrug‐resistant abilities (26/26). On the other hand, coinfection with APEC, H9 avian influenza virus (AIV) and Tembusu virus (TMUV) was very common on those farms (11/26), with APEC and TMUV sharing a similar morbidity peak (from May to September) and susceptibility (60% infections occurred in ducklings); thus, we speculated that the emerging TMUV infection escalated the APEC incidence in ducks. Finally, the data presented in this report enhance the current understanding of the epidemiology of APEC and different viral infections in ducks and provide additional insight into the critical factors that determine their pathogenicity. Meanwhile, the emergence of multidrug‐resistant APEC strains and their coinfection with different viruses emphasize that preventive measures against such infections on poultry farms should be implemented immediately.

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“…They have also been proven to play a role in distribution and spread of AMR (Deng et al, 2015). Several studies have reported a positive association between integrons and multidrug resistant E. coli isolates in food-producing animals (Cavicchio et al, 2015;Dou et al, 2016;Szmolka et al, 2015) as well as in humans (Lavakhamseh et al, 2016).…”

Section: Integronsmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Characterization of avian pathogenic Escherichia coli isolated in eastern China

Cited by 64 publications

References 23 publications

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

Duck viral infection escalated the incidence of avian pathogenic Escherichia coli in China

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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