Characterization of atypical Listeria innocua isolated from swine slaughterhouses and meat markets

Moreno, Luisa Zanolli; Paixão, Renata; Gobbi, Débora Dirani Sena de; Raimundo, Daniele Cristine; Ferreira, Tiago Osório; Hofer, Ernesto; Matté, Maria Helena; Moreno, Andréa Micke

doi:10.1016/j.resmic.2012.02.004

Cited by 37 publications

(32 citation statements)

References 16 publications

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“…Listeria innocua is generally considered to be nonpathogenic for humans. However, at least one human patient died after bacteraemia caused by a nonhaemolytic L. innocua (Perrin et al, 2003), and multiple L. innocua are haemolytic because they carry the prfA pathogenicity cluster (Johnson et al, 2004;Volokhov et al, 2007;Milillo et al, 2012;Moreno et al, 2012). We identified six strains from human infections that were L. innocua according to MLST.…”

Section: Discussionmentioning

confidence: 99%

The ubiquitous nature of Listeria monocytogenes clones: a large‐scale Multilocus Sequence Typing study

Haase

Didelot

Lecuit

et al. 2013

Environmental Microbiology

131

132

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Listeria monocytogenes is ubiquitously prevalent in natural environments and is transmitted via the food chain to animals and humans, in whom it can cause life-threatening diseases. We used Multilocus Sequence Typing (MLST) of ∼2000 isolates of L. monocytogenes to investigate whether specific associations existed between clonal complexes (CCs) and the environment versus diseased hosts. Most CCs (72%) were not specific for any single source, and many have been isolated from the environment, food products, animals as well as from humans. Our results confirm that the population structure of L. monocytogenes is largely clonal and consists of four lineages (I-IV), three of which contain multiple CCs. Most CCs have remained stable for decades, but one epidemic clone (CC101) was common in the mid-1950s and very rare until recently when it may have begun to re-emerge. The historical perspective used here indicates that the central sequence types of CCs were not ancestral founders but have rather simply increased in frequency over decades.

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Section: Discussionmentioning

confidence: 99%

The ubiquitous nature of Listeria monocytogenes clones: a large‐scale Multilocus Sequence Typing study

Haase

Didelot

Lecuit

et al. 2013

Environmental Microbiology

131

132

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“…Of these, 25 were isolated from pork, slaughterhouses, and markets (15 isolates of L. monocytogenes and 10 of L. innocua ), 11 isolates of L. monocytogenes were obtained from human infections, and four were control strains ( L. monocytogenes ATCC 19115 and ATCC 19111 and L. innocua ATCC 33090 and CLIP 12612) (Table 1). The environmental and pork isolates were isolated as described by Moreno et al [13]; the clinical strains and Listeria controls were obtained from the Public Health Laboratory (School of Public Health, University of Sao Paulo) and Laboratory of Swine Health (School of Veterinary Medicine and Animal Science, University of Sao Paulo) collections. The environmental and pork isolates were obtained from different swab samples taken from the slaughterhouses environment and carcasses from Sao Paulo State; the clinical isolates were obtained from the blood, placenta, and cerebrospinal fluid samples of different patients from different Brazilian states (Tables 1 and 2).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of AtypicalListeria monocytogenesandListeria innocuaIsolated from Swine Slaughterhouses and Meat Markets

Moreno

Paixão

Gobbi

et al. 2014

BioMed Research International

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In the last decade, atypical Listeria monocytogenes and L. innocua strains have been detected in food and the environment. Because of mutations in the major virulence genes, these strains have different virulence intensities in eukaryotic cells. In this study, we performed phenotypic and genotypic characterization of atypical L. monocytogenes and L. innocua isolates obtained from swine slaughterhouses and meat markets. Forty strains were studied, including isolates of L. monocytogenes and L. innocua with low-hemolytic activity. The isolates were characterized using conventional phenotypic Listeria identification tests and by the detection and analysis of L. monocytogenes-specific genes. Analysis of 16S rRNA was used for the molecular identification of the Listeria species. The L. monocytogenes isolates were positive for all of the virulence genes studied. The atypical L. innocua strains were positive for hly, plcA, and inlC. Mutations in the InlC, InlB, InlA, PI-PLC, PC-PLC, and PrfA proteins were detected in the atypical isolates. Further in vitro and transcriptomic studies are being developed to confirm the role of these mutations in Listeria virulence.

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“…The best-characterized L. monocytogenes virulence factors are listeriolysin O (LLO), encoded by the hly , and the internalins A and B (InlA and InlB) genes, respectively, which belong to the virulence gene cluster [18]. The present study detected hly gene in 6 out of 11 L. monocytogenes isolates with percentage (54.5%) as shown in photo (1).…”

Section: Resultsmentioning

confidence: 52%

Existence and Virulence Designation of Listeria Monocytogenes in Retail Chilled Pork Byproducts in Cairo Porcine Markets with Trials of Using Lactobacillus Probiotic as Anti-Listerial Meat Perservative

Abuelnaga¹,

Ata²,

Hedia³

et al. 2017

IOSR JESTFT

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Characterization of atypical Listeria innocua isolated from swine slaughterhouses and meat markets

Cited by 37 publications

References 16 publications

The ubiquitous nature of Listeria monocytogenes clones: a large‐scale Multilocus Sequence Typing study

The ubiquitous nature of Listeria monocytogenes clones: a large‐scale Multilocus Sequence Typing study

Phenotypic and Genotypic Characterization of AtypicalListeria monocytogenesandListeria innocuaIsolated from Swine Slaughterhouses and Meat Markets

Existence and Virulence Designation of Listeria Monocytogenes in Retail Chilled Pork Byproducts in Cairo Porcine Markets with Trials of Using Lactobacillus Probiotic as Anti-Listerial Meat Perservative

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