Characterization of an NDM-5 carbapenemase-producing Escherichia coli ST156 isolate from a poultry farm in Zhejiang, China

Tang, Biao; Chang, Jiang; Cao, Liujie; Luo, Qixia; Xu, Hao; Lyu, Wentao; Qian, Mingrong; Ji, Xiaofeng; Zhang, Qiaoyan; Xia, Xiaodong; Yang, Hua

doi:10.1186/s12866-019-1454-2

Cited by 40 publications

(34 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of carbapenem resistance in bacteria from animals, including food-producing animals (pigs, bovines and horses) has also been reported from some European countries such as Germany, France and Belgium [9]. The identification of E.coli isolates harbouring multiple (at least two) carbapenemase encoding genes from food animal in this study differentiates it from previous similar studies which mostly reported E. coli isolates harbouring one or two carbapenemase genes [11,12].…”

Section: Discussionsupporting

confidence: 60%

Phylogenetically Diverse Escherichia Coli Strains From Chicken Co-Harbour Multiple Carbapenemase Encoding Genes (blaNDM-blaOXA-blaIMP)

Aklilu¹,

Harun²,

Singh³

et al. 2020

Preprint

View full text Add to dashboard Cite

Carbapenem resistant Enterobacteriaceae (CRE) has been public health risk in several countries and recent reports indicate the emergence of CRE in food animals. This study was conducted to investigate the occurrence, resistance patterns, and phylogenetic diversity of CRE E.coli from chicken. Routine bacteriology, PCR detection of E.coli species, multiplex PCR to detect carbapenemase encoding genes and phylogeny of CRE E. coli were conducted. The results show that 24.36 % (19/78) were identified as CRE based on the phenotypic identifications of which 17 were positive for the tested carabanemase genes. The majority, 57.99% (11/19) of the isolates harbored multiple carbapenemase genes. Four isolates harbored all blaNDM blaOXA, blaIMP, five and two different isolates harbored blaNDM and blaOXA, and blaOXA and blaIMP respectively. The Meropenem, Imipenem and Ertapenem MIC values for the isolates ranged from 2g/mL to ≥256g/mL. Phylogenetic grouping showed that the CRE E.coli isolates belonged to five different groups; groups A, B1, C, D and unknown. The detection of carbapenem resistant E.coli in this study shows that CRE is has become an emerging problem in farm animals, particularly, in poultry farms. This also implies the potential public health risks posed by CRE from chicken to the consumers.

show abstract

Section: Discussionsupporting

confidence: 60%

Phylogenetically Diverse Escherichia Coli Strains From Chicken Co-Harbour Multiple Carbapenemase Encoding Genes (blaNDM-blaOXA-blaIMP)

Aklilu¹,

Harun²,

Singh³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…3). BLASTn analysis revealed high similarity (100% query cover and 99% nucleotide identity) to previously reported bla NDM−5 -positive plasmids, including the bla NDM−5 -positive plasmids of various sources, such as MH234502 (human, China) [9], CP027204 (sewage, China) [9], CP029245 (laying hen, China) [9], CP028705 (vegetable, China) [9], KX507346 (swine, China) [9], and CP034744 (goose, China) [9]. Comparative analysis revealed that these plasmids are highly similar, possessing the same backbone, which includes IncX3 replication.…”

Section: Conjugationsupporting

confidence: 51%

Characterization of carbapenem non-susceptible Gram-negative Bacilli isolated from the feces of 10,000 inpatients in Southern China

Duan¹,

Li²,

Peng³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Carbapenem non-susceptible Gram-negative bacilli (CNS-GNB) were dominant pathogen causing clinical infections. The human intestine was important reservoir of GNB, but there were few studies to analysis the prevalence of fecal colonization with them. Methods Fecal samples were collected from hosiptal screening test for GNB was conducted by using home-made MacConkey agar. Antimicrobial susceptibility was determined by the automatic microbiology analyzer and drug-resistant genes were characterized by polymerase chain reaction assays and DNA sequencing. The whole genome sequencing were used to analysis the characteristic of genetic structure of the isolates. Results A total of 680 CNS-GNB were collected. Acinetobacter spp. were the dominant species (33.8%) of the 22 genera. Carbapenemase genes were identified in 307 isolates (45.1%), including 206 (30.3%) blaNDM; 51 (7.5%) blaVIM−2, 48 (7.1%) blaIMP, and seven (1.0%) blaKPC−2. The blaNDM genes were first detected in three isolates, Providencia vermicola, Achromobacter spp., and Cupriavidus gilardii. Co-existence of blaVIM and blaIMP genes was detected in five isolates; Achromobacter co-producing VIM and IMP has not been previously reported. The mcr-1 gene was identified in five strains of Acinetobacter and one strain of K. pneumoniae. In addition, we detected seven isolates harboring the blaAFM−1 gene, a novel metallo-β-lactamase gene. This was first genomic analysis of ST11 K. pneumoniae co-producing NDM-5 and mcr-1, which revealed that blaNDM-5 and mcr-1 are located on two different plasmids. The plasmid harboring blaNDM-5, which was composed of a typical IncX3-type backbone, and the mcr-1 gene, was located between an IS30-like element ISApl1 and a PAP2-like encoding gene in the IncHI2-type plasmid. Conclusions the overall prevalence of fecal carriage of CNS-GNB in 10,000 stool samples was 7.45%, and more than half of CNS-GNB produced carbapenemase. Most CNS-GNB cases were associated with infectious disease, multiple hospitalizations, or long-term care, and a high prevalence of underlying disease.

show abstract

“…WGS showed that E. coli 15978 and C4109 belonged to ST156 and ST167, respectively ( Table 1). These two E. coli sequence types were also related to bla NDM dissemination worldwide in humans, 15,16 animals, 17,18 and food. 19 The resistance genes, chromosomal mutations, and virulence genes of the two isolates were displayed in Table 1.…”

Section: Resultsmentioning

confidence: 90%

Emergence of Almost Identical F36:A-:B32 Plasmids Carrying blaNDM-5 and qepA in Escherichia coli from Both Pakistan and Canada

Baloch¹,

Lv²,

Yi³

et al. 2019

IDR

View full text Add to dashboard Cite

The New Delhi Metallo-β-lactamase (NDM) producing Enterobacteriaceae is spreading worldwide. Although the bla NDM gene has been identified in animal associated Enterobacteriaceae isolates in many countries, little is known about its occurrence in animal products in Pakistan. In this study, 13 Escherichia coli isolates were collected from chicken meat samples in Pakistan. Two isolates, 15978 and C4109, exhibited reduced susceptibility (MIC ≥1 μg/mL) to imipenem, and carried bla NDM-5 and bla NDM-7 gene, respectively. Whole-genome sequencing and Oxford Nanopore MinION sequencing revealed that 15978 and C4109 belonged to ST156 and ST167, respectively. bla NDM-7 was carried by an IncX3 plasmid that has disseminated worldwide, whereas bla NDM-5 was located on an F36: A-: B32 plasmid, which shared high identity with two plasmids carried by E. coli isolates from other countries (one from a patient in Canada). To the best of our knowledge, this is the first report characterizing bla NDM -carrying plasmids from chicken meat samples in Pakistan. The dissemination of almost identical bla NDM-5 -bearing F36:A-:B32 and bla NDM-7 -bearing IncX3 plasmids in different countries highlights the importance of international trade and travel in the spread of antimicrobial resistance strains and plasmids worldwide.

show abstract

Characterization of an NDM-5 carbapenemase-producing Escherichia coli ST156 isolate from a poultry farm in Zhejiang, China

Cited by 40 publications

References 64 publications

Phylogenetically Diverse Escherichia Coli Strains From Chicken Co-Harbour Multiple Carbapenemase Encoding Genes (blaNDM-blaOXA-blaIMP)

Phylogenetically Diverse Escherichia Coli Strains From Chicken Co-Harbour Multiple Carbapenemase Encoding Genes (blaNDM-blaOXA-blaIMP)

Characterization of carbapenem non-susceptible Gram-negative Bacilli isolated from the feces of 10,000 inpatients in Southern China

<p>Emergence of Almost Identical F36:A-:B32 Plasmids Carrying <em>bla</em><sub>NDM-5</sub> and <em>qepA</em> in <em>Escherichia coli </em>from Both Pakistan and Canada</p>

Contact Info

Product

Resources

About