2006
DOI: 10.1128/aem.72.5.3515-3523.2006
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Abstract: A gene encoding an exo-␤-1,3-galactanase from Clostridium thermocellum, Ct1,3Gal43A, was isolated. The sequence has similarity with an exo-␤-1,3-galactanase of Phanerochaete chrysosporium (Pc1,3Gal43A). The gene encodes a modular protein consisting of an N-terminal glycoside hydrolase family 43 (GH43) module, a family 13 carbohydrate-binding module (CBM13), and a C-terminal dockerin domain. The gene corresponding to the GH43 module was expressed in Escherichia coli, and the gene product was characterized. The … Show more

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Cited by 44 publications
(61 citation statements)
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“…The activity of the recombinant enzyme was determined by the method described previously. 6,7) The reaction mixture consisted of 20 ml of McIlvaine buffer (0.2 M Na 2 HPO 4 -0.1 M citric acid), pH 5.0, and 25 ml of 1% (w/v) -1,3-galactan. After 5 min of preincubation at 37 C, 5 ml of enzyme was added to the solution, and the mixture was incubated at 37 C for 10 min.…”
Section: Methodsmentioning
confidence: 99%
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“…The activity of the recombinant enzyme was determined by the method described previously. 6,7) The reaction mixture consisted of 20 ml of McIlvaine buffer (0.2 M Na 2 HPO 4 -0.1 M citric acid), pH 5.0, and 25 ml of 1% (w/v) -1,3-galactan. After 5 min of preincubation at 37 C, 5 ml of enzyme was added to the solution, and the mixture was incubated at 37 C for 10 min.…”
Section: Methodsmentioning
confidence: 99%
“…Isopropylthiogalactopyranoside was added to 1 mM, and incubation was continued at 20 C for 24 h. The cells were harvested and sonicated by the method described previously. 7) After removal of insoluble material by centrifugation, the supernatants were used as crude enzymes. Each crude enzyme was purified with a Ni-NTA agarose (Qiagen, Hilden, Germany) column (5 Â 50 mm).…”
Section: Methodsmentioning
confidence: 99%
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