Characterization of an Elicitor-Induced Rice WRKY Gene,<i>OsWRKY71</i>

Chujo, Takeshi; Kato, Tomoaki; Yamada, Kazunari; Takai, Ryota; Akimoto-Tomiyama, Chiharu; Minami, Eiichi; Nagamura, Yoshiaki; Shibuya, Naoto; Yasuda, Michiko; Nakashita, Hideo; Umemura, Kenji; Okada, Atsushi; Okada, Kazunori; Nojiri, Hideaki; Yamane, Hisakazu

doi:10.1271/bbb.70553

Cited by 41 publications

(32 citation statements)

References 26 publications

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“…Among the 13 characterized rice WRKY proteins involved in rice-pathogen interactions, only six have been examined for their transcriptional activity in rice cells (Cheng and Wang, 2014). WRKY45-1/WRKY45 and WRKY53 show transactivation activity (Chujo et al, 2007;Shimono et al, 2007), whereas WRKY13, WRKY28, WRKY71, and WRKY76 are transcriptional repressors (Chujo et al, 2008Xiao et al, 2013;Yokotani et al, 2013). Interestingly, the four repressors belong to group II WRKYs, which harbor one WRKY motif and one C2H2-type zinc-finger motif, and WRKY53 and WRKY45-1/WRKY45 belong to group I WRKYs, which harbor two WRKY motifs and two C2H2-type zinc-finger motifs, and group III WRKYs, which harbor one WRKY motif and one C2HC-type zinc-finger motif, respectively, based on the classification of rice WRKY proteins (Wu et al, 2005).…”

Section: Wrky42 As a Transcriptional Repressor Negativelymentioning

confidence: 99%

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

et al. 2015

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Blast caused by fungal Magnaporthe oryzae is a devastating disease of rice (Oryza sativa) worldwide, and this fungus also infects barley (Hordeum vulgare). At least 11 rice WRKY transcription factors have been reported to regulate rice response to M. oryzae either positively or negatively. However, the relationships of these WRKYs in the rice defense signaling pathway against M. oryzae are unknown. Previous studies have revealed that rice WRKY13 (as a transcriptional repressor) and WRKY45-2 enhance resistance to M. oryzae. Here, we show that rice WRKY42, functioning as a transcriptional repressor, suppresses resistance to M. oryzae. WRKY42-RNA interference (RNAi) and WRKY42-overexpressing (oe) plants showed increased resistance and susceptibility to M. oryzae, accompanied by increased or reduced jasmonic acid (JA) content, respectively, compared with wild-type plants. JA pretreatment enhanced the resistance of WRKY42-oe plants to M. oryzae. WRKY13 directly suppressed WRKY42. WRKY45-2, functioning as a transcriptional activator, directly activated WRKY13. In addition, WRKY13 directly suppressed WRKY45-2 by feedback regulation. The WRKY13-RNAi WRKY45-2-oe and WRKY13-oe WRKY42-oe double transgenic lines showed increased susceptibility to M. oryzae compared with WRKY45-2-oe and WRKY13-oe plants, respectively. These results suggest that the three WRKYs form a sequential transcriptional regulatory cascade. WRKY42 may negatively regulate rice response to M. oryzae by suppressing JA signaling-related genes, and WRKY45-2 transcriptionally activates WRKY13, whose encoding protein in turn transcriptionally suppresses WRKY42 to regulate rice resistance to M. oryzae.

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Section: Wrky42 As a Transcriptional Repressor Negativelymentioning

confidence: 99%

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

et al. 2015

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“…In addition, the MAPK6-mediated cascade is activated by the RAC1 defensome complex to transduce PIT-mediated immunity (Lieberherr et al, 2005;Kawano et al, 2010). Similarly, the WRKY transcription factor family has been extensively studied for its role in the rice defense response (Liu et al, 2005;Liu et al, 2007;Qiu et al, 2007;Shimono et al, 2007;Chujo et al, 2008;Peng et al, 2008;Tao et al, 2009). Overexpression of WRKY53 induced after chitin treatment in cultured rice cells (Chujo et al, 2007) and in rice plants leads to enhanced resistance to M. oryzae, indicating its positive role in basal defense (Chujo et al, 2009).…”

Section: Hdt701 Regulation Of the Expression Of Defense-related Genesmentioning

confidence: 99%

HDT701, a Histone H4 Deacetylase, Negatively Regulates Plant Innate Immunity by Modulating Histone H4 Acetylation of Defense-Related Genes in Rice

et al. 2012

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Histone acetylation and deacetylation play an important role in the modification of chromatin structure and regulation of gene expression in eukaryotes. Chromatin acetylation status is modulated antagonistically by histone acetyltransferases and histone deacetylases (HDACs). In this study, we characterized the function of histone deacetylase701 (HDT701), a member of the plant-specific HD2 subfamily of HDACs, in rice (Oryza sativa) innate immunity. Transcription of HDT701 is increased in the compatible reaction and decreased in the incompatible reaction after infection by the fungal pathogen Magnaporthe oryzae. Overexpression of HDT701 in transgenic rice leads to decreased levels of histone H4 acetylation and enhanced susceptibility to the rice pathogens M. oryzae and Xanthomonas oryzae pv oryzae (Xoo). By contrast, silencing of HDT701 in transgenic rice causes elevated levels of histone H4 acetylation and elevated transcription of pattern recognition receptor (PRR) and defense-related genes, increased generation of reactive oxygen species after pathogen-associated molecular pattern elicitor treatment, as well as enhanced resistance to both M. oryzae and Xoo. We also found that HDT701 can bind to defense-related genes to regulate their expression. Taken together, these results demonstrate that HDT701 negatively regulates innate immunity by modulating the levels of histone H4 acetylation of PRR and defense-related genes in rice.

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“…Modeling the dynamics of the 2245 features across the two treatments and three time points allowed features to be grouped into six distinct expression profiles (profiles I to VI), of which the largest group, termed profile I, corresponded to 720 features that showed a strong increase in accumulation at 2 DAI with a reduction at the 4 and 6 DAI time points ( Figure 5B; see Supplemental Data Set 1 online). Within this category were a large proportion of genes representative of basal defense with some of them already well characterized during leaf infection (Table 1); for example, an endochitinase (Chit1; Kim et al, 2009), an elicitor-inducible shikimate kinase (SK2; Kasai et al, 2005), certain members of the WRKY family (WRKY71, Chujo et al, 2008;WRKY53, Chujo et al, 2009), or noteworthy, the previously described chitin receptor (chitin-elicitor binding protein, CEBiP; Kaku et al, 2006), a Ser/Thr protein kinase previously reported to be associated with defense against biotrophic rust fungi in other cereals (ORK10; Cheng et al, 2002b), and NAC4, a transcriptional activator involved in the initiation of hypersensitive response-associated cell death (Kaneda et al, 2009). The distinct gene expression pattern was independently confirmed by real-time RT-PCR (see Supplemental Figure 1 online).…”

Section: Root Invasion By M Oryzae Is Accompanied By Suppression Of mentioning

confidence: 99%

Tissue-Adapted Invasion Strategies of the Rice Blast Fungus Magnaporthe oryzae

et al. 2010

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Magnaporthe oryzae causes rice blast, the most serious foliar fungal disease of cultivated rice (Oryza sativa). During hemibiotrophic leaf infection, the pathogen simultaneously combines biotrophic and necrotrophic growth. Here, we provide cytological and molecular evidence that, in contrast to leaf tissue infection, the fungus adopts a uniquely biotrophic infection strategy in roots for a prolonged period and spreads without causing a loss of host cell viability. Consistent with a biotrophic lifestyle, intracellularly growing hyphae of M. oryzae are surrounded by a plant-derived membrane. Global, temporal gene expression analysis used to monitor rice responses to progressive root infection revealed a rapid but transient induction of basal defense-related gene transcripts, indicating perception of the pathogen by the rice root. Early defense gene induction was followed by suppression at the onset of intracellular fungal growth, consistent with the biotrophic nature of root invasion. By contrast, during foliar infection, the vast majority of these transcripts continued to accumulate or increased in abundance. Furthermore, induction of necrotrophy-associated genes during early tissue penetration, previously observed in infected leaves, was not seen in roots. Collectively, our results not only report a global characterization of transcriptional root responses to a biotrophic fungal pathogen but also provide initial evidence for tissue-adapted fungal infection strategies.

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Characterization of an Elicitor-Induced Rice WRKY Gene,OsWRKY71

Cited by 41 publications

References 26 publications

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

HDT701, a Histone H4 Deacetylase, Negatively Regulates Plant Innate Immunity by Modulating Histone H4 Acetylation of Defense-Related Genes in Rice

Tissue-Adapted Invasion Strategies of the Rice Blast Fungus Magnaporthe oryzae

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