Characterization of Alternanthera mosaic virus and its Coat Protein

Abstract

A new isolate of Alternanthera mosaic virus (AltMV-MU) was purified from Portulaca grandiflora plants. It has been shown that the AltMV-MU coat protein (CP) can be efficiently reassembled in vitro under different conditions into helical RNA-free virus-like particles (VLPs) antigenically related to native virus. The AltMV-MU and VLPs were examined by atomic force and transmission electron microscopies. The encapsidated AltMV-MU RNA is nontranslatable in vitro. However, it can be translationally activated by CP phosphorylation or by binding to the TGB1protein from the virus-coded movement triple gene block.

Study of the potexvirus ribonucleoproteins signal of assembly

“…Ранее в нашей лаборатории было показано, что БО ВМАльт способен к образованию протя-женных вирусоподобных частиц в отсутствии РНК в различных условиях [7]. Такая полимеризация БО ВМАльт затрудняет анализ формирования вРНП при инкубации БО ВМАльт с 5′-концевыми транскриптами РНК ВМАльт.…”

“…Данные по подавле-нию трансляции РНК в составе вРНП свидетель-ствуют о том, что в случае ВМАльт участок инициа-ции сборки также находится на 5′-конце молекулы РНК [6,7].…”

New phytoviral vector for superexpression of target proteins in plants

“…Èñ-ñëåäîâàíèå ïðåïàðàòîâ âèðóñîïîäîáíûõ ÷àñòèö ìå-òîäîì ïðîñâå÷èâàþùåé ýëåêòðîííîé ìèêðîñêîïèè ïðîâîäèëè, êàê îïèñàíî â ðàáîòå [6].…”

“…1, À). Áûëî ïîêàçàíî, ÷òî âûäåëåííûé èç ïðå-ïàðàòà AltMV-MU ÁÎ ñïîñîáåí îáðàçîâûâàòü ÂÏ× in vitro â îòñóòñòâèå âèðóñíîé ÐÍÊ [6].…”

The role of the 5′-cap structure in viral ribonucleoproteins assembly from potato virus X coat protein and RNAs

“…According to Lough et al [14], regulatory elements required for PVX reconstitution span the 5 0 -proximal 1-107-nt fragment of PVX RNA. It has been reported that the papaya mosaic potexvirus [16] and alternanthera mosaic potexvirus [17] assembly were also initiated at the 5 0 end of its RNA. 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 Previously, we have shown that in vitro incubation of PVX CP with heterologous RNAs of a number of plant and animal viruses belonging to various taxonomic groups produced viral ribonucleoproteins (vRNPs) whose morphological and translational properties were identical to those of cognate reassembled vRNP (PVX RNAePVX CP).…”