Characterization of a second gene (ZSOD22) of Na+/H+ antiporter from salt-tolerant yeastZygosaccharomyces rouxii and functional expression ofZSOD2 andZSOD22 inSaccharomyces cerevisiae

Iwaki, Toru; Higashida, Yuuichi; Tsuji, Hiromitsu; Tamai, Youichi; Watanabe, Yasuo

doi:10.1002/(sici)1097-0061(19980930)14:13<1167::aid-yea318>3.0.co;2-5

Cited by 57 publications

(70 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the nuclear-encoded SOD2 gene, we tried to clone two different SOD2 genes, previously characterized in Z. rouxii ATCC 42981 (Iwaki et al, 1998;Watanabe et al, 1991Watanabe et al, , 1995 and in the hybrid strain NCYC 1682 (James et al, 2005). The SOD1F/SOD1R primer pair was selected to amplify the Z. rouxii CBS 732 T ZrSOD2-22 gene and the SOD2F/SOD2R primer set was specifically designed to amplify the Z. rouxii ATCC 42981/NCYC3042 ZSOD22 variant.…”

Section: Cox2 Sod2 and His3 Gene Sequencingmentioning

confidence: 99%

See 1 more Smart Citation

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

Solieri

Cassanelli

Giudici

2007

Yeast

View full text Add to dashboard Cite

The taxonomic status and species number of the genus Zygosaccharomyces have rapidly changed in the last years. In this study, two new osmotolerant Zygosaccharomyces strains isolated from traditional balsamic vinegar, viz. ABT301 and ABT601, were investigated to elucidate their taxonomic relationships with Zygosaccharomyces rouxii species. A multi-gene sequence approach was employed, including regions of the rDNA repeat [5.8S, two internal transcribed spacers (ITS) and the 26S D1/D2 domain], COX2 mitochondrial gene and two nuclear genes (SOD2 and HIS3 ). Cloning and sequence analysis of 5.8S-ITS rDNA revealed that these strains bear an unusual polymorphism for this region. Three highly divergent 5.8S-ITS sequences were detected, one identical to Z. rouxii, the other two showing some relatedness to Z. mellis. Sequence and gene number polymorphism was also observed for the protein-encoding nuclear genes SOD2 and HIS3, as two copies for each gene different from those found in Z. rouxii were detected. Analysis of the D1/D2 26S domain showed that ABT301 and ABT601 have only one type of D1/D2 sequence statistically different from that of Z. rouxii. The findings obtained in this work suggest that the genomic background of strains ABT301 and ABT601 is different from the other Zygosaccharomyces species. We speculated that they could belong to a new putative species related to Z. rouxii.

show abstract

Section: Cox2 Sod2 and His3 Gene Sequencingmentioning

confidence: 99%

“…the Z. rouxii ATCC 42981 strain is considered haploid (Iwaki et al, 1998), even though it has two different SOD2 copies, but other Z. rouxii strains, such as CBS 732 T , are diploid (de Montigny et al, 2000;Sujaya et al, 2003). Such heterogeneity in the gene copy numbers and sequences (e.g.…”

Section: Protein-encoding Nuclear Genes Sod2 and His3mentioning

confidence: 99%

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

Solieri

Cassanelli

Giudici

2007

Yeast

View full text Add to dashboard Cite

show abstract

“…In addition to H + -ATPase, Na + \H + antiporters are known to play an important role in the maintenance and regulation of intracellular H + and Na + homeostasis (Padan & Schuldiner, 1994 ;Utsugi et al, 1998). This antiporter carries out cross-membrane electroneutral exchange of Na + and H + (Jia et al, 1992 ;Watanabe et al, 1995 ;Hahnenberger et al, 1997 ;Iwaki et al, 1998). The observed effect of Na + on the extrusion of H + and germtube formation in C. albicans indicates the existence of a Na + \H + antiporter(s) and their possible role in influencing the growth-mode transition.…”

Section: Introductionmentioning

confidence: 98%

“…T.-W. SOONG and OTHERS Na + \H + antiporter genes have been cloned and characterized from several fungi including Saccharomyces cerevisiae (NHA1 ; Prior et al, 1996), Schizosaccharomyces pombe (sod2 ; Jia et al, 1992) and Zygosaccharomyces rouxii (ZSOD2 and ZSOD22 ; Watanabe et al, 1995 ;Iwaki et al, 1998). However, no Na + \H + antiporter has so far been reported in C. albicans.…”

Section: Introductionmentioning

confidence: 99%

The Candida albicans antiporter gene CNH1 has a role in Na+ and H+ transport, salt tolerance, and morphogenesis The GenBank accession number for the sequence reported in this work is AF128238.

et al. 2000

View full text Add to dashboard Cite

The isolation and functional characterization of a Candida albicans Na M /H M antiporter gene, CNH1, is reported here. The gene encodes a protein of 840 amino acids that exhibits high levels of similarity in sequence, size, and structural and functional domains to a group of known Na M /H M antiporters of fungi. The CNH1 gene is able to functionally complement the salt-sensitivity of a Saccharomyces cerevisiae ena1 nha1 mutant, and mutations of two conserved aspartate residues to asparagines in the putative Na M -binding site abolish this activity. Deletion of CNH1 results in retardation of growth and a highly elongated morphology in a significant fraction of cells under conditions that normally support yeast growth. These results indicate that CNH1 has a role in Na M and H M transport, salt-tolerance, and morphogenesis.

show abstract

“…Therefore, cells have relatively high concentrations of K + and low concentrations of Na + , which are achieved by the coordinated functions of different transporters for cation influx and efflux. The NHA1 gene product has been described as a Na + /H + antiporter because it is involved in sodium tolerance and it has similarities to the Na + /H + antiporters sod2 of Schizosaccharomyces pombe (Jia et al, 1992), Z-Sod2 and Z-Sod22 of Zygosaccharomyces rouxii (Iwaki et al, 1998;Watanabe et al, 1995) and Cnh1 of Candida albicans (Soong et al, 2000). Nha1p has been shown to be involved in K + efflux through the plasma membrane, playing an important role during growth in a highpotassium environment (Bañ uelos et al, 1998;Kinclová et al, 2001).…”

mentioning

confidence: 99%

Role of the Nha1 antiporter in regulating K⁺ influx in Saccharomyces cerevisiae

Bañuelos¹,

Ruiz²,

Jiménez³

et al. 2001

Yeast

View full text Add to dashboard Cite

NHA1 encodes a K + (Na + )/H + antiporter in the plasma membrane of Saccharomyces cerevisiae. We report that cells expressing the NHA1 gene contained less K + than the mutant lacking the gene when grown without K + limitation. They also grew better at low K + and showed higher affinity of transport than the nha1 strain. In agreement with the function of an electroneutral cation/H + antiporter, the effect was only observed at acidic pH. The improved growth and transport depended on the presence of Trk1p (the main K + influx system) and did not require the product of TRK2. We propose that Nha1p regulates the potassium content of the cell and, as a consequence, can affect the activity of the main K + influx system (Trk1p).

show abstract

Characterization of a second gene (ZSOD22) of Na+/H+ antiporter from salt-tolerant yeastZygosaccharomyces rouxii and functional expression ofZSOD2 andZSOD22 inSaccharomyces cerevisiae

Cited by 57 publications

References 16 publications

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

The Candida albicans antiporter gene CNH1 has a role in Na+ and H+ transport, salt tolerance, and morphogenesis The GenBank accession number for the sequence reported in this work is AF128238.

Role of the Nha1 antiporter in regulating K⁺ influx in Saccharomyces cerevisiae

Contact Info

Product

Resources

About

Characterization of a second gene (ZSOD22) of Na+/H+ antiporter from salt-tolerant yeastZygosaccharomyces rouxii and functional expression ofZSOD2 andZSOD22 inSaccharomyces cerevisiae

Cited by 57 publications

References 16 publications

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

The Candida albicans antiporter gene CNH1 has a role in Na+ and H+ transport, salt tolerance, and morphogenesis The GenBank accession number for the sequence reported in this work is AF128238.

Role of the Nha1 antiporter in regulating K+ influx in Saccharomyces cerevisiae

Contact Info

Product

Resources

About

Role of the Nha1 antiporter in regulating K⁺ influx in Saccharomyces cerevisiae