Characterization of a parainfluenza virus isolated from a bottlenose dolphin (Tursiops truncatus)

Nollens, Hendrik H.; Wellehan, James F.X.; Saliki, Jeremiah T.; Caseltine, Shannon L.; Jensen, Eric D.; Bonn, William Van; Venn‐Watson, Stephanie

doi:10.1016/j.vetmic.2007.10.005

Cited by 33 publications

(39 citation statements)

References 36 publications

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“…The bovine parainfluenza virus 3 strain SF-4 was purchased from ATCC (VR-281). The dolphin isolate of parainfluenza virus (Tursiops truncatus PIV-1, or TtPIV-1) was obtained from the Marine Mammal Health Program at the University of Florida (28). Both BPIV-3 and TtPIV-1 were propagated in primary bovine turbinate (BT) cells.…”

Section: Methodsmentioning

confidence: 99%

Parainfluenza Virus 3 Blocks Antiviral Mediators Downstream of the Interferon Lambda Receptor by Modulating Stat1 Phosphorylation

Eberle

McGill

Reinhardt

et al. 2016

J Virol

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Parainfluenza viruses are known to inhibit type I interferon (IFN) production; however, there is a lack of information regarding the type III IFN response during infection. Type III IFNs signal through a unique heterodimeric receptor, IFN-R1/interleukin-10R2 (IL-10R2), which is primarily expressed by epithelial cells. Parainfluenza virus 3 (PIV-3) infection is highly restricted to the airway epithelium. We therefore sought to examine type III IFN signaling pathways during PIV-3 infection of epithelial cells. We used three strains of PIV-3: human PIV-3 (HPIV-3), bovine PIV-3 (BPIV-3), and dolphin PIV-1 (Tursiops truncatus PIV-1, or TtPIV-1). Here, we show that message levels of IL-29 are significantly increased during PIV-3 infection, yet downstream antiviral signaling molecules are not upregulated to levels similar to those of the positive control. IMPORTANCEParainfluenza virus (PIV) in humans is associated with bronchiolitis and pneumonia and can be especially problematic in infants and the elderly. Also seen in cattle, bovine PIV-3 causes respiratory infections in young calves. In addition, PIV-3 is one of a number of pathogens that contribute to the bovine respiratory disease complex (BRDC). As their name suggests, interferons (IFNs) are produced by cells to interfere with viral replication. Paramyxoviruses have previously been shown to block production and downstream signaling of type I IFNs. For the first time, it is shown here that PIV-3 can induce protective type III IFNs in epithelial cells, the primary site of PIV-3 infection. However, we found that PIV-3 modulates signaling pathways downstream of the type III IFN receptor to block production of several specific molecules that aid in a productive antiviral response. Importantly, this work expands our understanding of how PIV-3 effectively evades host innate immunity. P arainfluenza virus 3 (PIV-3) causes a prominent respiratory infection in both cattle and humans. The CDC reports that, in humans, most children have antibodies against human PIV-3 (HPIV-3) by 5 years of age (http://www.cdc.gov/parainfluenza /hcp/clinical.html). There is currently no vaccine available for control of HPIV-3 infection; however, a few studies have examined the use of an attenuated bovine PIV-3 (BPIV-3) to protect against HPIV-3 because of the homology between bovine and human strains (1-3). Given the lack of an efficacious vaccine for HPIV-3, there is a critical need to understand the mechanisms of HPIV-3-induced disease and the molecular pathways associated with viral modulation of the host antiviral defenses.Paramyoxviruses are negative-sense single-stranded RNA viruses which are part of the Paramyxoviridae family and Paramyxovirinae subfamily (4). PIV-3 is found within the genus Respirovirus. PIV-3 is a respiratory virus that primarily infects the epithelial cells of the lung. Symptoms of HPIV-3 infection include bronchiolitis and pneumonia, and HPIV-3 is especially problematic in infants (4). Airway epithelial cells recognize viral infection through pattern recog...

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Section: Methodsmentioning

confidence: 99%

Parainfluenza Virus 3 Blocks Antiviral Mediators Downstream of the Interferon Lambda Receptor by Modulating Stat1 Phosphorylation

Eberle

McGill

Reinhardt

et al. 2016

J Virol

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“…A large number of diverse host species have been involved, including avian, porcine, canine, bovine, equine, ovine, human, reptilian, and aquatic species (22,29,40,50,51). Cases of cross-species transmission and pathogen jumping to humans were also reported (10,20), demonstrating the value of characterizing new animal pathogens, even if their pathogenic potential is currently unknown.…”

mentioning

confidence: 99%

“…Hendra virus and Nipah virus, which caused severe respiratory disease and death in horses and their trainer and severe febrile encephalitis and death in pigs and farmers, respectively, have been classified as members of the genus Henipavirus in the subfamily Paramyxovirinae (7,9,20,30,48). Some recently isolated viruses, such as Menangle virus (55), Tupaia paramyxovirus (69), Tioman virus (11), Mossman virus (47), J-virus (31,33), Beilong virus (42), Mapuera virus (34), Tursiops truncatus parainfluenzavirus 1 (PIV1), isolated from bottlenose dolphins (50), and Atlantic salmon paramyxovirus (51), remain unclassified below the subfamily level. All members of the subfamily Paramyxovirinae have six genes in the following order: 3Ј-N-P-M-F-A-L-5Ј, where N, P, M, F, A, and L indicate the genes for the nucleocapsid protein, the phosphoprotein, and the matrix, fusion, attachment, and large polymerase proteins, respectively (40).…”

mentioning

confidence: 99%

Complete Genome Sequence and Pathogenicity of Two Swine Parainfluenzavirus 3 Isolates from Pigs in the United States

Qiao

Janke

Elankumaran

2010

J Virol

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“…Primers and protocols for the broad detection of herpesviruses, paramyxoviruses, and reoviruses were used as previously described (Vandevanter et al 1996, Nollens et al 2007, Wellehan et al 2009). …”

Section: Animals and Samplesmentioning

confidence: 99%

Detection of a respiratory coronavirus from tissues archived during a pneumonia epizootic in free-ranging Pacific harbor seals Phoca vitulina richardsii

Nollens¹,

Wellehan²,

Archer³

et al. 2010

Dis. Aquat. Org.

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In June 2000, 21 adult harbor seals Phoca vitulina richardsii were found dead along a localized section of the central California coast. Necropsy of 5 fresh carcasses revealed pulmonary congestion, consolidation, and hemorrhage. Histopathological changes in lungs from 2 of these seals included a necrotizing lymphocytic and histocytic lobar pneumonia with intra-lesional bacteria. A coronavirus (CoV) was detected in archived tissues from 1 of the 5 seals via a degenerate PCR for nidoviral RNA-dependent RNA polymerase (RdRp), and was subsequently confirmed via specific PCR. Based on the partial RdRp sequence, the CoV was identified as a novel, divergent member of the CoV group 1a. The virus is tentatively named harbor seal coronavirus (HSCoV). The clinical significance of HSCoV and its involvement in the etiology of the epizootic pneumonia and deaths of the harbor seals is uncertain. KEY WORDS: Harbor seal · Phoca vitulina richardsii · Coronavirus · Pneumonia Resale or republication not permitted without written consent of the publisherDis Aquat Org 90: [113][114][115][116][117][118][119][120] 2010 crochiropteran bat hosts; and Group 2d, from megachiropteran bat hosts (Woo et al. 2007). It has been proposed that Group 3 be divided as follows: Groups 3a, from avian hosts; Group 3b, which contains a coronavirus from a beluga whale Delphinapterus leucas, the only coronavirus identified in a marine mammal to date; and Group 3c, which contains viruses from diverse avian hosts as well as one from an Asian leopard cat Prionailurus bengalensis that may represent recent host switching (Woo et al. 2009).In late May and early June 2000, 21 adult harbor seals Phoca vitulina richardsii were found dead along a localized 10 mile (16 km) section of the central California coast at Point Reyes National Seashore. Upon postmortem examination of 5 relatively fresh carcasses, all seals had grossly abnormal lungs, and histological examination of 3 cases revealed severe pneumonia. A similar event with similar gross necropsy findings had occurred in 1997, when approximately 90 seals were found dead in the same area, but no conclusive etiology for the event was identified. In both events, a viral infection with secondary bacterial pneumonia was suspected. CoVs are recognized causes of enteric and respiratory infections that are often fatal in young animals. Here we report on the identification of a novel respiratory coronavirus from one of these harbor seals. This novel coronavirus of wildlife is a novel member of Group 1a and is distinct from, but most closely related to the CoVs of ferrets, cats, dogs, and swine. MATERIALS AND METHODS Animals and samples.On 26 May 2000, a harbor seal (HS D62) was found dead at McClure's Beach, Point Reyes National Seashore, in central California, and a field necropsy was conducted. Tonsil, stomach, cerebellum, brain stem, and lymph node were collected in 10% formalin for histological analysis. An additional 4 adult harbor seals were found dead on 6 June 2000. Two harbor seals, designated as H...

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Characterization of a parainfluenza virus isolated from a bottlenose dolphin (Tursiops truncatus)

Cited by 33 publications

References 36 publications

Parainfluenza Virus 3 Blocks Antiviral Mediators Downstream of the Interferon Lambda Receptor by Modulating Stat1 Phosphorylation

Parainfluenza Virus 3 Blocks Antiviral Mediators Downstream of the Interferon Lambda Receptor by Modulating Stat1 Phosphorylation

Complete Genome Sequence and Pathogenicity of Two Swine Parainfluenzavirus 3 Isolates from Pigs in the United States

Detection of a respiratory coronavirus from tissues archived during a pneumonia epizootic in free-ranging Pacific harbor seals Phoca vitulina richardsii

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