Characterization of a new coagulase-negative Staphylococcus species (Staphylococcus pettenkoferi) isolated from blood cultures from a hospitalized patient in Porto Alegre, Brazil

d’Azevedo, Pedro Alves; Comin, Guilherme; Cantarelli, Vlademir Vicente

doi:10.1590/s0037-86822010000300023

Cited by 11 publications

(10 citation statements)

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“…New Gram-positive cocci species have been identifi ed from clinical specimens in recent years, and these species exhibit phenotypic profi les similar to other staphylococcal strains 13 . Although these species were not included in our study, the tested strains allowed us to evaluate the accuracy of the automated system in identifying the common species isolated in the clinical setting.…”

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confidence: 99%

Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

Paim

Cantarelli

d’Azevedo

2014

Rev. Soc. Bras. Med. Trop.

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Introduction. The genera Enterococcus, Staphylococcus and Streptococcus are recognized as important Gram-positive human pathogens. The aim of this study was to evaluate the performance of Vitek 2 in identifying Gram-positive cocci and their antimicrobial susceptibilities. Methods. One hundred four isolates were analyzed to determine the accuracy of the automated system for identifying the bacteria and their susceptibility to oxacillin and vancomycin. Results. The system correctly identifi ed 77.9% and 97.1% of the isolates at the species and genus levels, respectively. Additionally, 81.8% of the Vitek 2 results agreed with the known antimicrobial susceptibility profi les. Conclusion. Vitek 2 correctly identifi ed the commonly isolated strains; however, the limitations of the method may lead to ambiguous fi ndings.Keywords: Gram-positive cocci. Vitek 2 automated system.Gram-positive cocci are widely distributed as part of the normal fl ora in humans; however, some species are recognized as major human pathogens and cause a large variety of infections worldwide. These microorganisms are frequently isolated from bloodstream infections, skin and soft tissue infections, sepsis, urinary tract infections and lower respiratory tract infections 1 .Automated bacterial identifi cation in the clinical laboratory provides a rapid and reliable diagnosis for most pathogens involved in infectious diseases. A previous study demonstrated the satisfactory performances of the automated methodologies, resulting in their use in routine practice with a highly acceptable level of identification accuracy; additionally, automated identification enabled the interpretation of antimicrobial susceptibility tests for the correct treatment of patients 2 .The aim of the present study was to evaluate the performance of the Vitek 2 automated system in the identifi cation of bacteria and antimicrobial susceptibilities of Gram-positive cocci isolates recovered from clinical samples and reference strains.The study was performed at Laboratório de Cocos GramPositivos (LCGP) of the Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA) and Laboratório Qualità, Rio Grande do Sul, Brazil. The isolates included in the present study were selected from the strain collection belonging LCGP. A total of 104 isolates of Gram-positive cocci were analyzed, including 29 reference strains selected from the American Type Culture Collection (ATCC) and 75 clinical strains isolated from different patients; these strains included Staphylococcus coagulase-negative (n=36), Enterococcus spp. (n=33) and Staphylococcus aureus (n=6). All of these strains have been previously characterized by the LCGP with regard to their virulence factors using molecular methods and susceptibility profi les and were identifi ed at the species level using conventional reference methods 3,4 . For the identifi cation of staphylococci, the following characteristics were tested: catalase; colony morphology and pigmentation; Gram stain; hemolysis; susceptibility to novobiocin; p...

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confidence: 99%

Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

Paim

Cantarelli

d’Azevedo

2014

Rev. Soc. Bras. Med. Trop.

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“…Because of these inconsistent biochemical findings, we proceeded to the sequencing of the 16S rRNA gene. The 16S rRNA genes from both isolates were amplified by PCR (Perkin-Elmer Gene-Amp PCR System 2720) using the universal primers 27F 5′-AGAGTTTGATCMTGGCTCAG-3′ and 1492R 5′-TACGGYTACCTTGTTACGACTT-3′ [8]. PCR products were purified and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems Warrington, UK) and the ABI Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, Calif, US).…”

Section: Case Presentationmentioning

confidence: 99%

“…Two strains have been described in 2007 in a French diabetic patient with osteomyelitis and in a case of bloodstream infection in Korea in 2009, respectively [6, 7]. In 2010, two further isolates have been identified from a patient hospitalized in Porto Alegre, Brazil [8]. …”

Section: Introductionmentioning

confidence: 99%

A Fatal Bloodstream Infection byStaphylococcus pettenkoferiin an Intensive Care Unit Patient

Mammina

Bonura

Verde

et al. 2011

Case Reports in Critical Care

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“…Three more S. pettenkoferi isolates from blood cultures were reported later [ 2 ]. Cases of osteomyelitis [ 3 ] and bloodstream infection [ 4 , 5 ] were thought to be caused by S. pettenkoferi . Isolates resistant to methicillin and linezolid were also reported recently [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Clinical and Microbiological Characteristics of Six Staphylococcus pettenkoferi Isolates From Blood Samples

2015

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Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Brucker Biotyper (Brucker Daltonics, Bremen, Germany) and molecular typing with 16S rRNA gene sequencing confirmed the 6 isolates as S. pettenkoferi. The conventional phenotypic identification of these isolates is not reliable owing to their inconsistent biochemical characteristics. Five of the 6 isolates were found to be resistant to oxacillin, and all isolates showed susceptibility to vancomycin and linezolid. For accurate identification of this novel species, advanced methods by using Brucker Biotyper or molecular methods such as 16S rRNA gene sequencing are required.

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Characterization of a new coagulase-negative Staphylococcus species (Staphylococcus pettenkoferi) isolated from blood cultures from a hospitalized patient in Porto Alegre, Brazil

Cited by 11 publications

References 5 publications

Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

A Fatal Bloodstream Infection byStaphylococcus pettenkoferiin an Intensive Care Unit Patient

Clinical and Microbiological Characteristics of Six Staphylococcus pettenkoferi Isolates From Blood Samples

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