Characterization of a Mr 25,000 basic fibroblast growth factor form in adult, regenerating, and fetal rat liver

Presta, Marco; Statuto, Massimo; Rusnati, Marco; Dell’Era, Patrizia; Ragnotti, Giovanni

doi:10.1016/0006-291x(89)91794-4

Cited by 42 publications

(29 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 148 amino acid form could not result from an internal initiation of the translation since there is no methionine or other unusual initiation codon near the cleavage site (SIT"TLP). The same truncated form was also observed in extracts of eukaryotic cells producing the 210FGF-2 form (data not shown) and was described as artefactual degradation occurring during storage of tissues at -20°C [28,33]. Futhermore, we and others never identified such a maturation product after the 155FGF-2 form production suggesting that the processing recognition site is not fully present in the 155 amino acid form [15,18,19].…”

Section: Pur$cation Of the R21ofgf-2supporting

confidence: 61%

“…Analysis of an extended hepatoma FGF-2 cDNA revealed unusual start codons [22]: proteins result from an alternative initiation of translation at an AUG (155FGF-2, 155 amino acids: 18 kDa) or at three inframe upstream CUGs (210FGF-2, 210 amino acids: 24 kDa, 201 amino acids: 22.5 kDa and 196 amino *Corresponding author. Fax: (33) 6226-4012. acids: 22 kDa) [22,23]. The high molecular weight forms of FGF-2 contain a glycine-arginine-rich domain where arginines can be dimethylated [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Purification and characterization of the 210‐amino acid recombinant basic fibroblast growth factor form (FGF‐2)

et al. 1994

View full text Add to dashboard Cite

Four forms of basic fibroblast growth factor (bFGF or FGF-2), using one AUG (155 amino acids) and three upstream CUG (210, 201 and 196 amino acids) start codons, were synthesized through an alternative use of initiation codons. The 210-amino acid form of FGF-2 (210FGF-2) was expressed in a plasmid vector under the control of a bacteriophage T7 RNA polymerase promoter system in Escherichia coli. Characterization of the purified protein was performed by electrospray mass spectrometry and Edman degradation. The recombinant 210FGF-2 produced in E. coli had a mitogenic activity similar to the 146-amino acid form extracted from tissues.

show abstract

Section: Pur$cation Of the R21ofgf-2supporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Purification and characterization of the 210‐amino acid recombinant basic fibroblast growth factor form (FGF‐2)

et al. 1994

View full text Add to dashboard Cite

show abstract

“…Hep ato cellular production of VEGF peaks 48-72 hours after hepatectomy, 20 as mitogens for hepatocytes FGF-2 are overexpressed in the regenerating liver. 21,22 It has been reported that FGF-2 stimulates the regeneration of the extracellular matrix after liver injury and regulates proliferation and migration of hepatocytes in vitro. 23,24 In the regenerating liver, FGF-2 seems to be primarily produced in hepatic stellate cells acting on the sinusoids.…”

Section: Discussionmentioning

confidence: 99%

Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice

et al. 2012

View full text Add to dashboard Cite

Background: Different approaches to study liver regeneration in murine models have been proposed. We investigated the effect of different liver resection models on liver damage and regeneration parameters in mice. Methods:We compared the technical aspect of the 2 most commonly used techniques of 50% and 70% liver resection. Liver damage, as determined by the change in serum alanine aminotransferase and aspartate aminotransferase, as well as the regeneration parameters VEGF and FGF-2 were analyzed at 6 time points. A postoperative vitality score was introduced.Results: Cholestasis was not observed for either technique. Both resection techniques resulted in full weight recovery of the liver after 240 hours, with no significant difference between sham and resection groups. Postoperative animal morbidity and total protein levels did not differ significantly for either method, indicating early and full functional recovery. However, comparing the mitogenic growth factors FGF-2 and VEGF, a significant increase in serum levels and, therefore, increased growth stimulus, was shown in the extended resection group. Conclusion:Extended resection led to a greater response in growth factor expression. This finding is important since it shows that growth factor response differs acdording to the extent of resection. We have demonstrated the need to standardize murine hepatic resection models to adequately compare the resulting liver damage.Contexte : Différentes approches ont été proposées pour étudier la régénérescence du foie dans des modèles murins. Nous avons exploré l'effet de divers modèles de résection sur l'atteinte hépatique et les paramètres de la régénérescence chez la souris. Méthodes :Nous avons comparé l'aspect technique des 2 approches les plus couramment utilisées, soit les résections de 50 % et de 70 % du foie. L'atteinte hépatique, déter-minée en fonction de la modification des taux d'alanine aminotransférase et d'aspartate aminotransférase sériques, ainsi que les paramètres de régénérescence VEGF (facteur de croissance endothéliale vasculaire) et FGF-2 (facteur de croissance des fibroblastes) ont été analysés à 6 moments. Un score de vitalité postopératoire a en outre été introduit.

show abstract

“…Although mRNA levels are very low, resting adult livers contain abundant reserves of FGF1 and FGF2 protein at levels higher than most other tissues (12). FGF1 and FGF2 mRNAs increase in response to partial hepatectomy or damage (13)(14)(15). Other FGF homologues have been reported in liver, but their significance, cellular target, and physiologic role are unclear (16).…”

Section: Introductionmentioning

confidence: 99%

Ectopic Activity of Fibroblast Growth Factor Receptor 1 in Hepatocytes Accelerates Hepatocarcinogenesis by Driving Proliferation and Vascular Endothelial Growth Factor–Induced Angiogenesis

Huang

Jin

et al. 2006

Cancer Research

View full text Add to dashboard Cite

Fibroblast growth factor (FGF) signaling mediates cell-to-cell communication in development and organ homeostasis in adults. Of the four FGF receptor (FGFR) tyrosine kinases, only FGFR4 is expressed in mature hepatocytes. Although FGFR1 is expressed by hepatic cell progenitors and adult nonparenchymal cells, ectopic expression is commonly observed in hepatoma cells. Here, we determined whether ectopic FGFR1 is a cause or consequence of hepatocellular carcinoma by targeting a constitutively active human FGFR1 to mouse hepatocytes. Livers of transgenic mice exhibited accelerated regeneration after partial hepatectomy but no signs of neoplastic or preneoplastic abnormalities for up to 18 months. However, in diethylnitrosamine-treated mice, the chronic FGFR1 activity promoted an incidence of 44% adenomas at 4 months and 38% hepatocellular carcinoma at 8 months. No adenoma or hepatocellular carcinoma was observed in diethylnitrosamine-treated wild-type (WT) livers at 4 or 8 months, respectively. At 10 and 12 months, tumorbearing livers in transgenic mice were twice the size of those in WT animals. Isolated hepatoma cells from the transgenic tumors exhibited a growth advantage in culture. Advanced hepatocellular carcinoma in the transgenic livers exhibited a reduced rate of necrosis. This was accompanied by a mean microvessel density of 2.7 times that of WT tumors and a markedly higher level of vascular endothelial growth factor. In cooperation with an initiator, the persistent activity of ectopic FGFR1 in hepatocytes is a strong promoter of hepatocellular carcinoma by driving cell proliferation at early stages and promoting neoangiogenesis at late stages of progression.

show abstract

Characterization of a Mr 25,000 basic fibroblast growth factor form in adult, regenerating, and fetal rat liver

Cited by 42 publications

References 9 publications

Purification and characterization of the 210‐amino acid recombinant basic fibroblast growth factor form (FGF‐2)

Purification and characterization of the 210‐amino acid recombinant basic fibroblast growth factor form (FGF‐2)

Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice

Ectopic Activity of Fibroblast Growth Factor Receptor 1 in Hepatocytes Accelerates Hepatocarcinogenesis by Driving Proliferation and Vascular Endothelial Growth Factor–Induced Angiogenesis

Contact Info

Product

Resources

About