Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production

Sigdel, Sujan; Singh, Ranjitha; Kim, Tae Su; Li, Jinglin; Kim, Sang Yong; Kim, In Won; Jung, Woo Hee; Pan, Cheol Ho; Lee, Jung-Kul

doi:10.1371/journal.pone.0131585

Cited by 17 publications

(10 citation statements)

References 49 publications

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“…Following transport of mannose into the cell, one pathway for mannose utilization is by isomerization to fructose; however, such isomerases are predominantly found in bacteria and do not exist in yeast ( Rajesh et al, 2012 ; Sigdel et al, 2015 ). Not surprisingly, BLAST analysis revealed no homologs of bacterial mannose isomerases in Y. lipolytica (all BLAST analysis performed under default parameters unless source is noted).…”

Section: Hexose Substratesmentioning

confidence: 99%

Alternative Substrate Metabolism in Yarrowia lipolytica

et al. 2018

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Recent advances in genetic engineering capabilities have enabled the development of oleochemical producing strains of Yarrowia lipolytica. Much of the metabolic engineering effort has focused on pathway engineering of the product using glucose as the feedstock; however, alternative substrates, including various other hexose and pentose sugars, glycerol, lipids, acetate, and less-refined carbon feedstocks, have not received the same attention. In this review, we discuss recent work leading to better utilization of alternative substrates. This review aims to provide a comprehensive understanding of the current state of knowledge for alternative substrate utilization, suggest potential pathways identified through homology in the absence of prior characterization, discuss recent work that either identifies, endogenous or cryptic metabolism, and describe metabolic engineering to improve alternative substrate utilization. Finally, we describe the critical questions and challenges that remain for engineering Y. lipolytica for better alternative substrate utilization.

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Section: Hexose Substratesmentioning

confidence: 99%

Alternative Substrate Metabolism in Yarrowia lipolytica

et al. 2018

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“…Considering that, in addition to gene/protein expression, enzyme activity can also affect their function and cellular response to drug treatment [34]. Cysteine carbazole sulfuric acid method was usually used to test enzyme activity of PMI [26]. First, we found that enzyme activity of PMI in 8305C and 8505C cells decreased when PMI was knocked down (Additional file 2: Fig.…”

Section: Intracellular Zn 2+ Concentration Affects Pmi Activity and The Response Of Thyroid Cancer Cells To Mannosementioning

confidence: 99%

“…Cysteine carbazole sulfuric acid method was used to measure enzyme activity of PMI as described previously [26]. In brief, cells were washed, and then lysed by three freeze-thaw cycles and Ultrasonic cracker on ice.…”

Section: Measurement Of Pmi Enzyme Activitymentioning

confidence: 99%

ZIP10 is a negative determinant for anti-tumor effect of mannose in thyroid cancer by activating phosphate mannose isomerase

Wang

et al. 2021

J Exp Clin Cancer Res

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Background Mannose, a natural hexose existing in daily food, has been demonstrated to preferentially inhibit the progression of tumors with low expression of phosphate mannose isomerase (PMI). However, its function in thyroid cancer still remains elusive. Methods MTT, colony formation and flow cytometry assays were performed to determine the response of thyroid cancer cells to mannose. Meanwhile, mouse models of subcutaneous xenograft and primary papillary thyroid cancer were established to determine in vivo anti-tumor activity of mannose. The underlying mechanism of mannose selectively killing thyroid cancer cells was clarified by a series of molecular and biochemical experiments. Results Our data demonstrated that mannose selectively suppressed the growth of thyroid cancer cells, and found that enzyme activity of PMI rather than its protein expression was negatively associated with the response of thyroid cancer cells to mannose. Besides, our data showed that zinc ion (Zn2+) chelator TPEN clearly increased the response of mannose-insensitive cells to mannose by inhibiting enzyme activity of PMI, while Zn2+ supplement could effectively reverse this effect. Further studies found that the expression of zinc transport protein ZIP10, which transport Zn2+ from extracellular area into cells, was negatively related to the response of thyroid cancer cells to mannose. Knocking down ZIP10 in mannose-insensitive cells significantly inhibited in vitro and in vivo growth of these cells by decreasing intracellular Zn2+ concentration and enzyme activity of PMI. Moreover, ectopic expression of ZIP10 in mannose-sensitive cells decrease their cellular response to mannose. Mechanistically, mannose exerted its anti-tumor effect by inhibiting cellular glycolysis; however, this effect was highly dependent on expression status of ZIP10. Conclusion The present study demonstrate that mannose selectively kills thyroid cancer cells dependent on enzyme activity of PMI rather than its expression, and provide a mechanistic rationale for exploring clinical use of mannose in thyroid cancer therapy.

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“…A cancrose tipo A corresponde a forma mais severa da doença, sendo tipicamente da Ásia e causada por Xanthomonas citri subsp. citri (XAC), possuindo a maior gama de hospedeiros cítricos, como por exemplo, a laranja doce (Citrus sinensis), limão (C. limon) e lima ácida (C. aurantifolia) (STALL;SEYMOUR, 1983). É a estirpe que está mais difundida no mundo, atingindo o Japão, Argentina, Brasil, Paraguai, China, entre outros países.…”

Section: O Cancro Cítricounclassified

“…Proudfoot e colaboradores (PROUDFOOT et al, 1994) definiram três tipos de PMIs com base no alinhamento da sequência e propriedades físico-químicas e cinéticas. As enzimas dos mamíferos e da maioria das bactérias pertencem às PMIs do Tipo I, que são proteínas monofuncionais homólogas que catalisam uma única reação de isomerização (PROUDFOOT et al, 1994;PROUDFOOT;PAYTON; (SIGDEL et al, 2015).…”

Section: Fosfomanose Isomeraseunclassified

Planejamento computacional e avaliação in vitro de novos potenciais inibidores da fosfomanose isomerase (PMI) de interesse para o controle do cancro cítrico

Barcelos¹

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Primeiramente, gostaria de agradecer aos meus pais, José Anselmo e Elmara, meus maiores exemplos. Mãe, seu cuidado е dedicação me deram, em alguns momentos, а esperança pаrа seguir. Pai, sua presença significou segurança е certeza de que não estou sozinha nessa caminhada.Não poderia deixar de agradecer ao meu avô, Paulo, que todos os dias me levava para escola e jamais permitiu que eu faltasse.Ao meu irmão, Lucas, por todo amor e carinho. Aos meus padrinhos, tios, primos e avó que sempre estiveram presentes. Ao meu namorado, Rafael, por todo amor, carinho, paciência e compreensão que tem me dedicado e também pelas risadas nos momentos de aflição.Ao professor Carlos Henrique que, com muita paciência e atenção, dedicou seu valioso tempo para me orientar em cada passo desse trabalho e também pela contribuição em minha vida acadêmica e influência na minha futura vida profissional.Aos meus colegas de laboratório do LCQF (Leonardo, João Gabriel, Suzane, Guilherme, Raí, Isaque e Lorane) e ao André do LBBMA. Obrigada pelos momentos de paciência, de brincadeira e, principalmente, pela mão que se estendia quando eu precisava.Às minhas amigas e irmãs, Leticia, Julia, Marília e Patrícia por todo apoio e cumplicidade, porque mesmo distantes estavam presentes em minha vida.Às coordenadoras Joze e Cristiane Colégio Objetivo de Batatais por todos conselhos, apoio, torcida, risadas, companheirismo e amizade. Aos alunos, professores e funcionários do Colégio, o meu muito obrigada.Obrigada a todos que, mesmo não citados aqui, tanto contribuíram para a conclusão desta etapa e para quem eu sou hoje.

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Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production

Cited by 17 publications

References 49 publications

Alternative Substrate Metabolism in Yarrowia lipolytica

Alternative Substrate Metabolism in Yarrowia lipolytica

ZIP10 is a negative determinant for anti-tumor effect of mannose in thyroid cancer by activating phosphate mannose isomerase

Planejamento computacional e avaliação in vitro de novos potenciais inibidores da fosfomanose isomerase (PMI) de interesse para o controle do cancro cítrico

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Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production

Cited by 17 publications

References 49 publications

Alternative Substrate Metabolism in Yarrowia lipolytica

Alternative Substrate Metabolism in Yarrowia lipolytica

ZIP10 is a negative determinant for anti-tumor effect of mannose in thyroid cancer by activating phosphate mannose isomerase

Planejamento computacional e avaliação in vitro de novos potenciais inibidores da fosfomanose isomerase (PMI) de interesse para o controle do cancro cí­trico

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Planejamento computacional e avaliação in vitro de novos potenciais inibidores da fosfomanose isomerase (PMI) de interesse para o controle do cancro cítrico