Characterization of a lignin peroxidase gene from the white-rot fungus Trametes versicolor

Regulation of Laccase Gene Transcription in Trametes versicolor

“…This gene encodes a Cu-Zn superoxide dismutase which is regulated by copper and zinc. We have analyzed the promoter sequences upstream of the TATA boxes in lcc genes from a number of other white rot fungi (20,30,34,48,55) and can identify no sequences similar to the ACE1 binding site of S. cerevisiae SOD1 or to the MREs of higher eucaryotes. This leads us to speculate that the mechanism involved in the copper regulation of lcc transcription is unrelated to the mechanisms which mediate the regulation of metallothionein gene expression.…”

“…An XRE has been identified in the promoter of a lip gene (46); it conforms to the XRE consensus sequence, TCACGC (19). We have analyzed a number of white rot fungal lcc promoters (6,20,30,34,48,55) and have found putative XREs in two of them. The lcc promoter in the basidiomycete PM1 (6), a closely related fungus to T. versicolor, contains a sequence identical to the XRE consensus 180 bp upstream of the TATA box.…”

“…PCR amplification. Forward (5Ј-ATTGGCACGGCTTCTTCC-3Ј) and reverse (5Ј-GATCTGGATGGAGTCGAC-3Ј) PCR primers were based on conserved regions between previously described lcc sequences from Trametes species and closely related fungi (6,30,34,55). The predicted sizes for lcc amplification products from genomic DNA and cDNA were approximately 690 and 520 bp, respectively.…”

Regulation of Laccase Gene Transcription in Trametes versicolor

“…This gene encodes a Cu-Zn superoxide dismutase which is regulated by copper and zinc. We have analyzed the promoter sequences upstream of the TATA boxes in lcc genes from a number of other white rot fungi (20,30,34,48,55) and can identify no sequences similar to the ACE1 binding site of S. cerevisiae SOD1 or to the MREs of higher eucaryotes. This leads us to speculate that the mechanism involved in the copper regulation of lcc transcription is unrelated to the mechanisms which mediate the regulation of metallothionein gene expression.…”

“…An XRE has been identified in the promoter of a lip gene (46); it conforms to the XRE consensus sequence, TCACGC (19). We have analyzed a number of white rot fungal lcc promoters (6,20,30,34,48,55) and have found putative XREs in two of them. The lcc promoter in the basidiomycete PM1 (6), a closely related fungus to T. versicolor, contains a sequence identical to the XRE consensus 180 bp upstream of the TATA box.…”

“…PCR amplification. Forward (5Ј-ATTGGCACGGCTTCTTCC-3Ј) and reverse (5Ј-GATCTGGATGGAGTCGAC-3Ј) PCR primers were based on conserved regions between previously described lcc sequences from Trametes species and closely related fungi (6,30,34,55). The predicted sizes for lcc amplification products from genomic DNA and cDNA were approximately 690 and 520 bp, respectively.…”

Disulfide Bonds and Glycosylation in Fungal Peroxidases

“…(PCL7) is shown for comparison, as its crystal structure was recently solved Edwards et al, 1993;Piontek et al, 1993). T versicolor lignin isoperoxidase (TvLP12) has been cloned (Jonsson and Nyman, 1992) and characterized by partial amino-acid sequencing (Johansson et al, 1993). As the complete sequence of TvLPlO which was used in the present study is unknown, residue numbers of TvLP12 are used for reference.…”

“…Amino-acid sequences and M , of disulfide-linked peptides from TvLPlO and TvMP2, before and after reduction and carboxymethylation. Residue numbers for TvLPlO refer to the DNA-derived sequence for mature TvLPl2 (Jonsson and Nyman, 1992), and TvMP2 to a recent DNA-derived sequence (Fig. 2;Johansson, 1994).…”

Regulation of Laccase Gene Transcription in Trametes versicolor

“…This gene encodes a Cu-Zn superoxide dismutase which is regulated by copper and zinc. We have analyzed the promoter sequences upstream of the TATA boxes in lcc genes from a number of other white rot fungi (20,30,34,48,55) and can identify no sequences similar to the ACE1 binding site of S. cerevisiae SOD1 or to the MREs of higher eucaryotes. This leads us to speculate that the mechanism involved in the copper regulation of lcc transcription is unrelated to the mechanisms which mediate the regulation of metallothionein gene expression.…”

“…An XRE has been identified in the promoter of a lip gene (46); it conforms to the XRE consensus sequence, TCACGC (19). We have analyzed a number of white rot fungal lcc promoters (6,20,30,34,48,55) and have found putative XREs in two of them. The lcc promoter in the basidiomycete PM1 (6), a closely related fungus to T. versicolor, contains a sequence identical to the XRE consensus 180 bp upstream of the TATA box.…”

“…PCR amplification. Forward (5Ј-ATTGGCACGGCTTCTTCC-3Ј) and reverse (5Ј-GATCTGGATGGAGTCGAC-3Ј) PCR primers were based on conserved regions between previously described lcc sequences from Trametes species and closely related fungi (6,30,34,55). The predicted sizes for lcc amplification products from genomic DNA and cDNA were approximately 690 and 520 bp, respectively.…”

Disulfide Bonds and Glycosylation in Fungal Peroxidases

“…(PCL7) is shown for comparison, as its crystal structure was recently solved Edwards et al, 1993;Piontek et al, 1993). T versicolor lignin isoperoxidase (TvLP12) has been cloned (Jonsson and Nyman, 1992) and characterized by partial amino-acid sequencing (Johansson et al, 1993). As the complete sequence of TvLPlO which was used in the present study is unknown, residue numbers of TvLP12 are used for reference.…”

“…Amino-acid sequences and M , of disulfide-linked peptides from TvLPlO and TvMP2, before and after reduction and carboxymethylation. Residue numbers for TvLPlO refer to the DNA-derived sequence for mature TvLPl2 (Jonsson and Nyman, 1992), and TvMP2 to a recent DNA-derived sequence (Fig. 2;Johansson, 1994).…”

Regulation of Laccase Gene Transcription in Trametes versicolor

“…This gene encodes a Cu-Zn superoxide dismutase which is regulated by copper and zinc. We have analyzed the promoter sequences upstream of the TATA boxes in lcc genes from a number of other white rot fungi (20,30,34,48,55) and can identify no sequences similar to the ACE1 binding site of S. cerevisiae SOD1 or to the MREs of higher eucaryotes. This leads us to speculate that the mechanism involved in the copper regulation of lcc transcription is unrelated to the mechanisms which mediate the regulation of metallothionein gene expression.…”

“…An XRE has been identified in the promoter of a lip gene (46); it conforms to the XRE consensus sequence, TCACGC (19). We have analyzed a number of white rot fungal lcc promoters (6,20,30,34,48,55) and have found putative XREs in two of them. The lcc promoter in the basidiomycete PM1 (6), a closely related fungus to T. versicolor, contains a sequence identical to the XRE consensus 180 bp upstream of the TATA box.…”

“…PCR amplification. Forward (5Ј-ATTGGCACGGCTTCTTCC-3Ј) and reverse (5Ј-GATCTGGATGGAGTCGAC-3Ј) PCR primers were based on conserved regions between previously described lcc sequences from Trametes species and closely related fungi (6,30,34,55). The predicted sizes for lcc amplification products from genomic DNA and cDNA were approximately 690 and 520 bp, respectively.…”

Disulfide Bonds and Glycosylation in Fungal Peroxidases

“…(PCL7) is shown for comparison, as its crystal structure was recently solved Edwards et al, 1993;Piontek et al, 1993). T versicolor lignin isoperoxidase (TvLP12) has been cloned (Jonsson and Nyman, 1992) and characterized by partial amino-acid sequencing (Johansson et al, 1993). As the complete sequence of TvLPlO which was used in the present study is unknown, residue numbers of TvLP12 are used for reference.…”

“…Amino-acid sequences and M , of disulfide-linked peptides from TvLPlO and TvMP2, before and after reduction and carboxymethylation. Residue numbers for TvLPlO refer to the DNA-derived sequence for mature TvLPl2 (Jonsson and Nyman, 1992), and TvMP2 to a recent DNA-derived sequence (Fig. 2;Johansson, 1994).…”