Characterization of a lectin from the craysfish Cherax quadricarinatus hemolymph and its effect on hemocytes

Sánchez-Salgado, José Luis; Pereyra, Mohamed Alí; Vivanco-Rojas, Óscar; Sierra-Castillo, Claudia; Alpuche, Juan; Zenteno, Edgar; Agundis, Concepción

doi:10.1016/j.fsi.2014.05.039

Cited by 19 publications

(6 citation statements)

References 45 publications

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“…After homogenization, the suspension of each well was loaded into a well of 96-wells-plate to be read on a spectrophotometer (Multiskan Ascent, Thermo Labsystems, PHL, USA) at 630 nm. The O 2 - was determined as nanomoles (nM) of reduced NBT (NBTr) per well, considering an extinction factor of 0.1 OD at 630 nm representing 1.9 nM O 2 - 69 . The O 2 - production generated by the neutrophils without stimulus in fresh medium was considered the 100% of ROS production.…”

Section: Methodsmentioning

confidence: 99%

Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6

Magaña‐Guerrero¹,

Domínguez-López²,

Martinez-Aboytes³

et al. 2017

Sci Rep

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The mesenchymal stem cells obtained from human amniotic membrane (hAMSC) possess immunosuppressive functions through soluble factors such as prostanoids and proteins; thus, they have been proposed to ameliorate inflammatory processes. On the other hand, activated neutrophils are cells of the first line of immune defense that are able to release extracellular traps (NETs). NETs are formed of DNA and granular components; however, the excessive release of NETs is associated with the development of autoimmune and chronic inflammatory diseases. In this study, we identified that conditioned medium (CM) from hAMSC was able to diminish NETs release, as well as the production of reactive oxygen species (ROS) and the mitochondrial membrane potential from LPS-stimulated mouse bone marrow-derived neutrophils (BMN). Interestingly, NETs inhibition, ROS levels decrease and mitochondrial membrane potential loss were reverted when LPS-stimulated murine derived BMN were exposed to the CM from hAMSC transfected with TSG-6-siRNA. Finally, rhTSG6 was able to significantly diminish NETs release in BMN. These data suggest an inhibition mechanism of NETs ROS-dependent in which TSG-6 participates. Consequently, we propose the hAMSC use as a therapeutic candidate in the treatment of inflammatory diseases in which NETs are involved.

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Section: Methodsmentioning

confidence: 99%

Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6

Magaña‐Guerrero¹,

Domínguez-López²,

Martinez-Aboytes³

et al. 2017

Sci Rep

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“…Klebsiellapneumoniae 2 500 (Ravindranath et al, 1985;Muraliet al, 1999;Maheswari et al, 2002;Alpucheet al, 2005;Sun et al, 2008;Sánchez-Salgado et al, 2014). In our study, the serum hemagglutinating activity was screened with 11 different vertebrate erythrocytes, with 10 RBC showing agglutination with various titres.…”

Section: Salmonella Abortusequi 2 25mentioning

confidence: 77%

Physico-Chemical Characterization of a naturally occurring Hemagglutinin in the Serum of Sand Lobster, Thenus orientalis with Affinity for N-Acetylated Aminosugars

Vaishnavi¹,

Mullainadhan²

2017

Int.J.Curr.Microbiol.App.Sci

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“…Erythrocytes from human, rabbit and weather loach were used for the hemagglutination assay, following the procedure described in a previous study (Sanchez‐Salgado et al., 2014). The erythrocytes were collected with anticoagulant buffer (0.14 M NaCl, 0.1 M glucose, 30 mM trisodium citrate, 26 mM citric acid, 10 mM EDTA, pH 4.6) and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Zhang

Yang

Zhang³

et al. 2020

Journal of Fish Diseases

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C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a galactose‐specific QPD motif and a conserved Ca2+‐binding site. Transcripts of MaCTL were significantly upregulated after immune challenge with its pathogen A. hydrophila. In vitro assays with recombinant MaCTL protein revealed that it exhibited hemagglutinating and bacterial agglutinating activities, in a Ca2+‐dependent manner. MaCTL was found to bind to a wide range of bacteria, as well as bind to bacterial polysaccharides LPS and PGN. Moreover, MaCTL displayed antimicrobial activity by inhibiting the growth of bacteria. These results collectively suggest that MaCTL is involved in the antibacterial defence of weather loach.

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Characterization of a lectin from the craysfish Cherax quadricarinatus hemolymph and its effect on hemocytes

Cited by 19 publications

References 45 publications

Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6

Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6

Physico-Chemical Characterization of a naturally occurring Hemagglutinin in the Serum of Sand Lobster, Thenus orientalis with Affinity for N-Acetylated Aminosugars

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

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