Characterization of a human epidermis model reconstructed from hair follicle keratinocytes and comparison with two commercially models and native skin

Guiraud, B.; Hernandez-Pigeon, H.; Ceruti, Isabelle; Mas, Sergi; Palvadeau, Y.; Saint-Martory, C.; Castex‐Rizzi, N.; Duplan, Hélène; Bessou‐Touya, S.

doi:10.1111/ics.12150

Cited by 22 publications

(38 citation statements)

References 25 publications

(56 reference statements)

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“…After 14 days culture at the air/liquid interface, the RHE model presented a similar morphology and three-dimensional architecture to those of native epidermis with the presence of the classical four skin layers (stratum basale, stratum spinosum, stratum granulosum and the outermost layer stratum corneum). The RHE model reconstruction was reproducible and the expression of proliferation and differentiation markers was close to the marketed epidermal substitutes and skin [16]. Moreover, the hair follicle-derived RHE is a convenient and easy-to-handle in vitro RHE model and a more reliable source of biological material than skin explants.…”

Section: Introductionmentioning

confidence: 70%

“…Tissue engineering was performed in-house according to the recently published protocol [16]. The 1.12 cm 2 epidermal equivalent consisted of airlifted, living, multilayered, normal human keratinocytes cultured for 16 days on an inert polycarbonate filter.…”

Section: Preparation Of Reconstituted Human Epidermis and Skin Explantsmentioning

confidence: 99%

“…Therefore, we propose in the present work a strategy for the evaluation of the cutaneous protection against the genotoxicity of simulated sunlight using a recently characterized reconstituted human epidermis (RHE) grown from outer root sheath keratinocytes isolated from adult hair follicles [16]. This RHE model has the main advantage to be prepared from a non-invasive procedure since the keratinocytes are easily and quickly taken by hair plucking.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection

Bacqueville

Douki

Duprat

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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Section: Introductionmentioning

confidence: 70%

Section: Preparation Of Reconstituted Human Epidermis and Skin Explantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection

Bacqueville

Douki

Duprat

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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“…In line with this, very recently Bacqueville et al (2017) reported that COMT mRNA was strongly expressed in their ORS-RHE model (Guiraud et al 2014). …”

Section: Xenobiotica-metabolizing Enzymes In the Human Skin Includingmentioning

confidence: 66%

Xenobiotica-metabolizing enzymes in the skin of rat, mouse, pig, guinea pig, man, and in human skin models

2018

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Studies on the metabolic fate of medical drugs, skin care products, cosmetics and other chemicals intentionally or accidently applied to the human skin have become increasingly important in order to ascertain pharmacological effectiveness and to avoid toxicities. The use of freshly excised human skin for experimental investigations meets with ethical and practical limitations. Hence information on xenobiotic-metabolizing enzymes (XME) in the experimental systems available for pertinent studies compared with native human skin has become crucial. This review collects available information of which—taken with great caution because of the still very limited data—the most salient points are: in the skin of all animal species and skin-derived in vitro systems considered in this review cytochrome P450 (CYP)-dependent monooxygenase activities (largely responsible for initiating xenobiotica metabolism in the organ which provides most of the xenobiotica metabolism of the mammalian organism, the liver) are very low to undetectable. Quite likely other oxidative enzymes [e.g. flavin monooxygenase, COX (cooxidation by prostaglandin synthase)] will turn out to be much more important for the oxidative xenobiotic metabolism in the skin. Moreover, conjugating enzyme activities such as glutathione transferases and glucuronosyltransferases are much higher than the oxidative CYP activities. Since these conjugating enzymes are predominantly detoxifying, the skin appears to be predominantly protected against CYP-generated reactive metabolites. The following recommendations for the use of experimental animal species or human skin in vitro models may tentatively be derived from the information available to date: for dermal absorption and for skin irritation esterase activity is of special importance which in pig skin, some human cell lines and reconstructed skin models appears reasonably close to native human skin. With respect to genotoxicity and sensitization reactive-metabolite-reducing XME in primary human keratinocytes and several reconstructed human skin models appear reasonably close to human skin. For a more detailed delineation and discussion of the severe limitations see the Conclusions section in the end of this review.

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“…Another possibility is to seed the keratinocytes onto a de‐epidermised dermis derived from normal skin . A further option is to use keratinocytes derived from the outer root sheath of hair follicles to prepare skin models . This provides a non‐invasive way to generate skin models with keratinocytes derived from patients.…”

Section: D Skin Modelsmentioning

confidence: 99%

Skin microbiota and human 3D skin models

Rademacher

Simanski

Gläser

et al. 2018

Experimental Dermatology

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Although the role of the microbiota in skin homeostasis is still emerging, there is growing evidence that an intact microbiota supports the skin barrier. The increasing number of research efforts that are trying to shed more light on the human skin-microbiota interaction requires the use of suitable experimental models. Three-dimensional (3D) skin equivalents have been established as a valuable tool in dermatological research because they contain a fully differentiated epidermal barrier that reflects the morphological and molecular characteristics of normal human epidermis. In this review, we provide an overview of current 3D skin models and illustrate the potential of 3D skin models to study the human skin-microbiota interplay.

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Characterization of a human epidermis model reconstructed from hair follicle keratinocytes and comparison with two commercially models and native skin

Abstract: ORS cell-based reconstructed epidermis is a valid and reproducible model for human epidermis and it may be used to evaluate the effects of active substances and cosmetic formulations.

Cited by 22 publications

References 25 publications

A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection

A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection

Xenobiotica-metabolizing enzymes in the skin of rat, mouse, pig, guinea pig, man, and in human skin models

Skin microbiota and human 3D skin models

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