Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.)  in Tobacco (Nicotiana tabacum L.)

Iskandar, Hayati Minarsih; Suhandono, Sonny; Pambudi, Jembar; Kristianti, Tati; Putranto, Riza Arief; Mose, Windi; Sustiprijatno, Sustiprijatno

doi:10.29244/jtcs.7.01.28-36

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…It showed that Pr-1DHNSo can be considered a constitutive promoter based on functional tests previously conducted (Minarsih et al, 2020). The study of DHN promoter also indicated that the Pr-1DHNSo promoter from sugarcane was expressed and induced by drought stress treatment in tobacco plants (Iskandar et al, 2020).…”

Section: Introductionmentioning

confidence: 92%

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Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets

Minarsih

Fitriyah

Aksa

et al. 2023

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Dehydrin is known to have an important role in plant response and adaptation to abiotic stresses including drought and high salinity. Previous research reported the isolation of the full-length coding sequence (CDS) of DHN1 from sugarcane var. PSJT 941, and it shares a high homology with DHN genes from sorghum and other sugarcane varieties. In this study, the full-length CDS was cloned under the constitutive CaMV35S promoter and transformed into sugarcane calli mediated by Agrobacterium tumefaciens. The DHN promoter, Pr-1DHNSo, was also successfully isolated from the sugarcane var. PSJT 941 and cloned into the pBI121 expression vector. The promoter construct was subsequently transformed into sugarcane calli of var. Kidang Kencana. Transgenic sugarcane carrying DHN1 gene and DHN promoter constructs were regenerated according to the standard protocol of sugarcane tissue culture. Optimization of an acclimatization protocol using modified post-rooting media was also conducted and the resulting protocol reduced the total mortality rates of the transformed plantlets. The presence of the gene and promoter constructs was periodically tested by PCR using specific primers. The genotyping results showed that the constructs were present for more than a year after transformation.

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Section: Introductionmentioning

confidence: 92%

“…Prior research also showed that the activity of the sugarcane DHN promoter that controls the expression of the GUS promoter gene was greatly increased in drought-stressed transgenic tobacco plants, relative to well-watered tobacco plants (Iskandar et al, 2020).…”

Section: Transformation Of Pbi121-pr-1dhnso In Sugarcane Calli and It...mentioning

confidence: 99%

Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets

Minarsih

Fitriyah

Aksa

et al. 2023

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“…Basic fertilizers are given at the end of land preparation, while additional fertilizers are administered during plant growth. This additional fertilization is essential, specifically for plants with long growth phases, such as tomatoes (Maillard et al, 2015), tobacco (Iskandar et al, 2020), cauliflower (Sofian & Susila, 2018), and winged bean (Ishthifaiyyah et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effect of Leaf Pruning and Additional Fertilizer on Growth and Young Pods Yield of Winged Beans

Tustiyani,

Melati,

Arifin Aziz

et al. 2024

JTAS

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Winged bean (Psophocarpus tetragonolobus L.) exhibits luxuriant foliage, making leaf pruning essential to enhance sunlight interception. Additionally, supplementing with additional fertilizer helps offset the impact of gradual harvesting. Therefore, this research aimed to determine the effect of leaf pruning and additional fertilizer on the growth and yield parameter of winged beans in the Institut Pertanian Bogor (IPB) experimental station at Leuwikopo, IPB University, Bogor, Indonesia. A randomized complete block design was used with two factors and three replications, namely leaf pruning intensities (0, 15, and 30% leaf pruning) and rates of additional fertilizer (0, 6.25, 12.5, and 18.5 g NPK 16-16-16/plant). The observed variables were plant height, leaf number, root length, leaf nutrient, auxin content, nutrient uptake, and young pods yield. The findings revealed that the interaction of pruning intensities and additional fertilizer rates significantly influenced leaf number and root length. Specifically, plants receiving a treatment combination without pruning and 6.25 g of additional fertilizer/plant exhibited the highest leaf number. In contrast, those subjected to 15% leaf pruning showed the greatest root length. Leaf nutrient levels, auxin content, and nutrient uptake exhibited noticeable improved with the addition of fertilizer. Meanwhile, a higher phosphorus and organic carbon content was observed in the 15% pruning treatment, and the young pods yield were not affected because the Fairuz variety reached its potential yield. Based on the results, the most recommended treatment is a combination without leaf pruning, supplemented with 6.25 g of additional fertilizer/plant.

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Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.) in Tobacco (Nicotiana tabacum L.)

Cited by 2 publications

References 15 publications

Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets

Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets

Effect of Leaf Pruning and Additional Fertilizer on Growth and Young Pods Yield of Winged Beans

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