Characterization of 16 microsatellite markers for the <i>Oreinotinus</i> clade of <i>Viburnum</i> (Adoxaceae)

Barish, Syndi; Arakaki, Mónica; Edwards, Erika J.; Donoghue, Michael J.; Clement, Wendy L.

doi:10.3732/apps.1600103

Cited by 3 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…gSSRs have been developed for four of the~163 species of Viburnum including V. dilatatum [41], a member of the Succotinus subclade, V. rufidulum [42], a member of the Lentago subclade, and V. triphyllum and V. pichinchense [43], which are members of the Oreinotinus subclade [21]. These gSSRs were developed for species/cultivar identification as well as investigation of population genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of 15 Novel Genomic SSRs for Viburnum farreri

Hamm

Nowicki

Boggess

et al. 2021

Plants

View full text Add to dashboard Cite

The Viburnum genus is of particular interest to horticulturalists, phylogeneticists, and biogeographers. Despite its popularity, there are few existing molecular markers to investigate genetic diversity in this large genus, which includes over 160 species. There are also few polymorphic molecular tools that can delineate closely related species within the genus. Viburnum farreri, a member of the Solenotinus subclade and one of the centers of diversity for Viburnum, was selected for DNA sequencing and development of genomic simple sequence repeats (gSSRs). In this study, 15 polymorphic gSSRs were developed and characterized for a collection of 19 V. farreri samples. Number of alleles per locus ranged from two- to- eight and nine loci had four or more alleles. Observed heterozygosity ranged from 0 to 0.84 and expected heterozygosity ranged from 0.10 to 0.80 for the 15 loci. Shannon diversity index values across these loci ranged from 0.21 to 1.62. The markers developed in this study add to the existing molecular toolkit for the genus and will be used in future studies investigating cross-transferability, genetic variation, and species and cultivar delimitation in the Viburnum genus and closely allied genera in the Adoxaceae and Caprifoliaceae.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and Characterization of 15 Novel Genomic SSRs for Viburnum farreri

Hamm

Nowicki

Boggess

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…A total of 49 primer pairs from four previous studies that were developed from the four major clades of Viburnum were used in this study. Eleven markers were developed from V. dilatatum in Laminotinus 28 , seven from V. rufidulum in Valvatotinus 29 , 16 from V. triphyllum and V. pichinchense in Porphyrotinus 30 , and 15 from V. farreri in Crenotinus 31 (Supplementary Table 2 ). All SSR markers were single locus and polymorphic in the species from which they were obtained and included simple-perfect and compound-imperfect motifs.…”

Section: Methodsmentioning

confidence: 99%

“…All SSR markers were single locus and polymorphic in the species from which they were obtained and included simple-perfect and compound-imperfect motifs. For simplicity, the markers developed from V. triphyllum and V. pichinchense were renamed Vore01-16 (Vore = Viburnum Oreinotinus subclade) in the same order found in the published table (original names in parentheses in Supplementary Table 2 ) 30 .…”

Section: Methodsmentioning

confidence: 99%

“…Four sets of microsatellite markers, also known as simple sequence repeat (SSR) markers, were developed from species within the four major clades of Viburnum 22 including Laminotinus ( Succotinus subclade) 28 , Valvatotinus ( Lentago subclade) 29 , Porphyrotinus ( Oreinotinus subclade) 30 , and Crenotinus ( Solenotinus subclade) 31 (Fig. 1 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A set of SSR markers to characterize genetic diversity in all Viburnum species

Hamm

Nowicki

Boggess

et al. 2023

Sci Rep

View full text Add to dashboard Cite

About 160 species are classified within the Viburnum genus and many of these are cultivated for horticultural purposes. The vast dispersal of Viburnum makes the genus a useful model for studying evolutionary history and inferring how species expanded into their current distributions. Simple sequence repeat (SSR) markers were previously developed for five Viburnum species that were classified within the four major clades (Laminotinus, Crenotinus, Valvatotinus, and Porphyrotinus). The ability of some of these markers to cross-amplify in Viburnum species has been scantly evaluated, but there has not been any genus-wide assessment for the markers. We evaluated a collection of 49 SSR markers for the ability to cross-amplify in 224 samples, including 46 Viburnum species, representing all 16 subclades, and five additional species in the Viburnaceae and Caprifoliaceae. A subset of 14 potentially comprehensive markers for Viburnum species was identified and evaluated for the ability to detect polymorphisms in species outside of their respective clades. The 49 markers had overall amplification success in 52% of the samples, including a 60% success rate within the Viburnum genus and 14% in other genera. The comprehensive marker set amplified alleles in 74% of all samples tested, including 85% of Viburnum samples and 19% of outgroup samples. To the best of our knowledge, this is the first comprehensive set of markers able to characterize species across an entire genus. This set of markers can be used to assess the genetic diversity and population structure of most Viburnum species and closely allied species.

show abstract

“…Besides, plant morphology, taxonomical values of the phytochemical amentoflavone isolated from Viburnum leaves and branchlets [19], as well as anatomical characteristics of fruits, cork, assimilating parenchyma, and leaf epidermal cells [20,21], were also assessed. More recently, assessments of Viburnum diversification have been made at a molecular level [3,4,8,22,23]. Clement et al [24] proposed a Viburnum phyloge-netic classification and provided formal phylogenetic definitions for 30 clades.…”

Section: Introductionmentioning

confidence: 99%

Genus Viburnum: Therapeutic Potentialities and Agro‐Food‐Pharma Applications

Sharifi‐Rad

Quispe

Valdés

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

The genus Viburnum (Adoxaceae, Dipsacales) is of scientific interest due to the chemical components and diverse biological activities found across species of the genus, which includes more than 230 species of evergreen, semievergreen, or deciduous shrubs and small trees. Although frequently used as an ornament, the Viburnum species show biological properties with health-promoting effects. Fruits, flowers, and barks of certain species are used for pharmaceutical purposes or as cooking ingredients, hence containing biochemical compounds with health-promoting activity such are carotenoids, polyphenols, and flavonoids. However, its taxonomical determination is difficult, due to its wide distribution and frequent hybridizations; therefore, an objective classification would allow us to understand its biological activity based on its phytochemical components. More than sixty phytochemical compounds have been reported, where vibsanin-type diterpenes and their derivatives are the most prevalent. Leaves and twigs of V. dilatatum contain the largest number of phytochemicals among the genus. Through preclinical evidence, this study provides insight regarding antioxidant, antibacterial, anti-inflammatory, cytotoxic, and anticancer activities of genus Viburnum.

show abstract

Characterization of 16 microsatellite markers for the Oreinotinus clade of Viburnum (Adoxaceae)

Cited by 3 publications

References 13 publications

Development and Characterization of 15 Novel Genomic SSRs for Viburnum farreri

Development and Characterization of 15 Novel Genomic SSRs for Viburnum farreri

A set of SSR markers to characterize genetic diversity in all Viburnum species

Genus Viburnum: Therapeutic Potentialities and Agro‐Food‐Pharma Applications

Contact Info

Product

Resources

About