Characterization, Isolation, and Culture of Mouse and Human Spermatogonial Stem Cells

Guo, Ying; Hai, Yanan; Gong, Yuehua; Li, Zheng; He, Zuping

doi:10.1002/jcp.24471

Cited by 52 publications

(42 citation statements)

References 98 publications

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“…2 Lin28 was used as a SSCs marker in the previous studies and has been shown to be associated with stemness of SSCs. 36, 37, 38, 39, 40 In our experiments, Lin28 was used to distinguish the undifferentiated spermatogonia from other types of cells(such as Sertoli cell and differentiated spermatogonia).The result showed that Lin28-positive cells in ESET-KD group were significantly reduced (Figure 2e), suggesting ESET deficiency led to decrease of the number of SSCs probably because of apoptosis or differentiation.…”

Section: Resultsmentioning

confidence: 78%

The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice

Zhang

Qin

et al. 2014

Cell Death Dis

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Self-renewal and differentiation of spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout a male's life. SSC transplantation will be a valuable solution for young male patients to preserve their fertility. As SSCs in the collected testis tissue from the patients are very limited, it is necessary to expansion the SSCs in vitro. Previous studies suggested that histone methyltransferase ERG-associated protein with SET domain (ESET) represses gene expression and is essential for the maintenance of the pool of embryonic stem cells and neurons. The objective of this study was to determine the role of ESET in SSCs using in vitrocell culture and germ cell transplantation. Cell transplantation assay showed that knockdown of ESET reduced the number of seminiferous tubules with spermatogenesis when compared with that of the control. Knockdown of ESET also upregulated the expression of apoptosis-associated genes (such as P53, Caspase9, Apaf1), whereas inhibited the expression of apoptosis-suppressing genes (such as Bcl2l1, X-linked inhibitor of apoptosis protein). In addition, suppression of ESET led to increase in expression of Caspase9 and activation of Caspase3 (P17) as well as cleavage of poly (ADP-ribose) polymerase. Among the five ESET-targeting genes (Cox4i2, spermatogenesis and oogenesis Specific Basic Helix-Loop-Helix 2, Nobox, Foxn1 and Dazl) examined by ChIP assay, Cox4i2 was found to regulate SSC apoptosis by the rescue experiment. BSP analyses further showed that DNA methylation in the promoter loci of Cox4i2was influenced by ESET, indicating that ESET also regulated gene expression through DNA methylation in addition to histone methylation. In conclusion, we found that ESET regulated SSC apoptosis by suppressing of Cox4i2 expression through histone H3 lysine 9 tri-methylation and DNA methylation. The results obtained will provide unique insights that would broaden the research on SSC biology and contribute to the treatment of male infertility.

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Section: Resultsmentioning

confidence: 78%

The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice

Zhang

Qin

et al. 2014

Cell Death Dis

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“…The expression profile of miRNAs in testes is given in Table 1. miRNA and spermatogonial stem cell renewal Within the testis, the SSCs reside in a unique microenvironment, or 'niche', which includes the surrounding somatic cells. Spermatogenesis originates from SSCs, which have the dual property of continually renewing and undergoing differentiation into a spermatogonial progenitor that expands and further differentiates (Hess et al 2006, Ventela et al 2012, Dovere et al 2013, Silvan et al 2013, van den Driesche et al 2014, Guo et al 2014. In the rodent testis, SSCs are among undifferentiated spermatogonia that include Asingle (As), Apaired (Apr), and Aaligned (Aal) spermatogonia (Dym 1994).…”

Section: Expression Profile Of Mirna In the Testismentioning

confidence: 99%

Role of microRNAs in mammalian spermatogenesis and testicular germ cell tumors

Wang¹,

Xu²

2015

Reproduction

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microRNAs (miRNAs) are a class of small endogenous RNAs, 19-25 nucleotides in size, which play a role in the regulation of gene expression at transcriptional and post-transcriptional levels. Spermatogenesis is a complex process through which spermatogonial stem cells (SSCs) proliferate and differentiate into mature spermatozoa. A large number of miRNAs are abundantly expressed in spermatogenic cells. Growing evidence supports the essential role of miRNA regulation in normal spermatogenesis and male fertility and cumulative research has shown that this form of regulation contributes to the etiology of testicular germ cell tumors (TGCTs). In this review, we addressed recent advancements of miRNA expression profiles in testis and focused on the regulatory functions of miRNA in the process of SSC renewal, spermatogonial mitosis, spermatocyte meiosis, spermiogenesis, and the occurrence of TGCTs.

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“…Additionally, mGSCs were obtained from mouse testes, and they are morphologically similar to embryonic stem cells (ESCs) and contribute to embryonic development following microinjection into blastocysts (Oatley et al 2006;Dym et al 2009;He et al 2010). A long or short term culture system for mGSCs has been established for human, mouse, and other mammals (Akhondi et al 2013;Guo et al 2014). The cultured mGSCs also express the markers of SSCs, and differentiated into spermatozoa.…”

Section: Introductionmentioning

confidence: 99%