Characterization, biomedical and agricultural applications of protease inhibitors: A review

Response of Midgut Trypsin- and Chymotrypsin-Like Proteases of Helicoverpa armigera Larvae Upon Feeding With Peanut BBI: Biochemical and Biophysical Characterization of PnBBI

“…On the other hand, PIs are known to play a role in the regulation of programed cell death and tolerance against abiotic stress (Drame et al, 2013;Boex-fontvieille et al, 2015). Several studies demonstrated the use of PIs in the management of agriculturally important insect pests (Shamsi et al, 2016;War et al, 2018;Clemente et al, 2019). In this regard, serine PIs such as Kunitz and Bowman-Birk inhibitors (BBIs) are receiving considerable attention as a part of Integrated Pest Management (IPM) since lepidopteran insect pests depend predominantly on trypsin-and chymotrypsin-like digestive proteases for attaining energy (Sharma et al, 2000;Srinivasan et al, 2006;Swathi et al, 2016).…”

Identification of Tight-Binding Plasmepsin II and Falcipain 2 Inhibitors in Aqueous Extracts of Marine Invertebrates by the Combination of Enzymatic and Interaction-Based Assays

“…Consequently, protease inhibitors have become irreplaceable molecules not only as biochemical tools for the basic study of proteases, but also as drugs for the treatment of numerous chronic and infectious human diseases [2]. In addition, protease inhibitors have found applications in many other fields, including biotechnology, agriculture and food industries [5]. …”

Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes

“…Proteinaceous PIs in plants may help in regulating and keeping a check on endogenous proteases and in acting as protective agents against insect and/or microbial proteases. [1] In living cells, protease signaling pathways are strictly regulated, and the dysregulation of protease activity can lead to pathologies, important being cancer. The dysregulated proteases cause the dysregulation of cell cycle, apoptosis, cell growth and activation, cell-cell adhesion, cellular interactions, and signal transduction.…”

“…This is a multistep process in case of humans and animals involving various events of adherence, migration, and degeneration of extracellular matrix, and establishment of cancerous cells at the new site. [1] Metastasis of cancer cells requires action of proteases including serine and matrix metalloproteinases (MMP) family that functionally comprises complex interacting protease cascade system. [3] MMPs along with activated trypsin are coexpressed to facilitate cell invasion and metastasis.…”

“…[12] At present, phytodrugs are a promising complementary and alternative medicine in health care for treatment of diseases like cancer. Our study reveals antineoplastic and antioxidant activity of TIs from three different plant sources to examine their suppressive effects on normal and cancerous cell lines.…”