Characterization and Schwann Cell Seeding of up to 15.0 cm Long Spider Silk Nerve Conduits for Reconstruction of Peripheral Nerve Defects

Kornfeld, Tim; Vogt, Peter M.; Bucan, Vesna; Peck, Claas‐Tido; Reimers, Kerstin; Radtke, Christine

doi:10.3390/jfb7040030

Cited by 24 publications

(29 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results indicates that nerve regeneration through the spider silk nerve implant is at least as effective as autologous nerve grafts. The spider silk conduit was designed and prior to application in large animal model extensively tested in vitro and in small animal models in vivo [ 82 – 86 ]. Especially results of the current in vitro investigation of the spider silk based nerve implant emphasize that the implant might be suitable for nerve reconstruction on defect sides extending 6.0 cm in length [ 82 ].…”

Section: Current Experimental Approaches In Peripheral Nerve Reconstrmentioning

confidence: 99%

“…The spider silk conduit was designed and prior to application in large animal model extensively tested in vitro and in small animal models in vivo [ 82 – 86 ]. Especially results of the current in vitro investigation of the spider silk based nerve implant emphasize that the implant might be suitable for nerve reconstruction on defect sides extending 6.0 cm in length [ 82 ]. Outcome of previous the in vitro study are currently being reevaluated in the ovine animal model [ 82 ].…”

Section: Current Experimental Approaches In Peripheral Nerve Reconstrmentioning

confidence: 99%

“…Especially results of the current in vitro investigation of the spider silk based nerve implant emphasize that the implant might be suitable for nerve reconstruction on defect sides extending 6.0 cm in length [ 82 ]. Outcome of previous the in vitro study are currently being reevaluated in the ovine animal model [ 82 ]. Results of these in vivo investigation are pending.…”

Section: Current Experimental Approaches In Peripheral Nerve Reconstrmentioning

confidence: 99%

See 2 more Smart Citations

Nerve grafting for peripheral nerve injuries with extended defect sizes

Kornfeld

Vogt

Radtke

2018

Wien Med Wochenschr

Self Cite

172

151

View full text Add to dashboard Cite

Summary Artificial and non-artificial nerve grafts are the gold standard in peripheral nerve reconstruction in cases with extensive loss of nerve tissue, particularly where a direct end-to-end suture or an autologous nerve graft is inauspicious. Different materials are marketed and approved by the US Food and Drug Administration (FDA) for peripheral nerve graft reconstruction. The most frequently used materials are collagen and poly(DL-lactide-ε-caprolactone). Only one human nerve allograft is listed for peripheral nerve reconstruction by the FDA. All marketed nerve grafts are able to demonstrate sufficient nerve regeneration over small distances not exceeding 3.0 cm. A key question in the field is whether nerve reconstruction on large defect lengths extending 4.0 cm or more is possible. This review gives a summary of current clinical and experimental approaches in peripheral nerve surgery using artificial and non-artificial nerve grafts in short and long distance nerve defects. Strategies to extend nerve graft lengths for long nerve defects, such as enhancing axonal regeneration, include the additional application of Schwann cells, mesenchymal stem cells or supporting co-factors like growth factors on defect sizes between 4.0 and 8.0 cm.

show abstract

Section: Current Experimental Approaches In Peripheral Nerve Reconstrmentioning

confidence: 99%

Section: Current Experimental Approaches In Peripheral Nerve Reconstrmentioning

confidence: 99%

Section: Current Experimental Approaches In Peripheral Nerve Reconstrmentioning

confidence: 99%

See 1 more Smart Citation

Nerve grafting for peripheral nerve injuries with extended defect sizes

Kornfeld

Vogt

Radtke

2018

Wien Med Wochenschr

Self Cite

172

151

View full text Add to dashboard Cite

show abstract

“…Treatment of nerve defect injuries by autologous nerve transplantation represents the gold standard when a tension-free end-to-end coaptation is not achievable. However, with regard to donor site morbidity, nerve availability is limited [2,6,7,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…In the last years, many studies focused on finding valid alternatives. Nerve conduits made of biodegradable materials were developed as guiding channels for regenerating axons and redirect proximal nerve growth [2,4,6,7,8,9,10]. In some cases, conduits were filled with hydrogels or similar materials or neurotrophic factors to further improve and enhance recovery in peripheral nerve gap injuries.…”

Section: Introductionmentioning

confidence: 99%

Co-Culturing Human Adipose Derived Stem Cells and Schwann Cells on Spider Silk—A New Approach as Prerequisite for Enhanced Nerve Regeneration

Resch

Wolf

Mann

et al. 2018

IJMS

Self Cite

View full text Add to dashboard Cite

Fast recovery is crucial for a successful nerve repair and an optimal functional outcome after peripheral nerve injury. Regarding donor site morbidity, autologous transplantation shows great limitations, which urge the need for alternative options in nerve reconstruction. Spider silk was reported as an advantageous material for cell adhesion, migration and proliferation, and its use in conduits is of great interest, especially in combination with cells to improve nerve regeneration. We here described the behavior of a co-culture of human Schwann cells and human adipose-derived stem cells (ADSCs) on spider silk as a new approach. After characterized by immunostaining ADSCs and Schwann cells were seeded in the co-culture on a spider silk scaffold and observed for 21 days. Results showed that cells were attached to the silk and aligned along the silk fibers. With further culture time, cells migrated along the silk and increased in number and formed an almost confluent cell layer. In immunostaining, results suggest that the cell layer was equally composed of ADSCs and Schwann cells. In conclusion, we showed that by providing a guiding structure for directed growth and cells to support nerve regeneration and remyelination, a valid alternative to autologous nerve grafts could have been found.

show abstract

Nerve Growth Factor‐Binding Engineered Silk Films Promote Neuronal Attachment and Neurite Outgrowth

Baker

Akher

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

Spider silks have outstanding potential as biomaterials due to their soughtafter mechanical properties and low immunogenicity. The toughest spider silk is aciniform silk, which is used by spiders to wrap prey and produce egg sacs. A variety of recombinant aciniform silk constructs are now been developed, including hybrid silks with domains from multiple spider silk proteins fused together. In this study, an engineered aciniform silk construct, termed N B Silk, fused both N-and C-terminally to heptapeptide motifs that bind the neurotrophic factor and nerve growth factor-β (NGF) is introduced. N B Silk is shown to be amenable to casting into robust films that remain intact while sequestering and maintaining bioactive NGF at the film surface for at least 7 days. These films support cell survival while enhancing differentiation and neurite density and outgrowth in neuron-like PC12 cells, with elevation of signaling through both the mitogen-activated protein kinase (MAPK) and protein kinase B (AKT) signaling pathways. Strikingly, preloading of N B Silk films with NGF enhances neuritogenesis even over conditions where cells are grown with NGF supplementation in the culture medium. N B Silk scaffolds, thus, warrant future development and evaluation as biomaterials for nerve regeneration.

show abstract

Characterization and Schwann Cell Seeding of up to 15.0 cm Long Spider Silk Nerve Conduits for Reconstruction of Peripheral Nerve Defects

Cited by 24 publications

References 50 publications

Nerve grafting for peripheral nerve injuries with extended defect sizes

Nerve grafting for peripheral nerve injuries with extended defect sizes

Co-Culturing Human Adipose Derived Stem Cells and Schwann Cells on Spider Silk—A New Approach as Prerequisite for Enhanced Nerve Regeneration

Nerve Growth Factor‐Binding Engineered Silk Films Promote Neuronal Attachment and Neurite Outgrowth

Contact Info

Product

Resources

About