Characterization and postmortem diagnosis of fatal heatstroke using Attenuated Total Reflectance Fourier transform infrared spectroscopy combined with chemometrics

Tuo, Ya; Zhang, Kai; Wang, Lei; Luo, Yang; Sun, Qiran; Lin, Hancheng; Zhang, Zhong; Chen, Yijiu; Sun, Jun-hong; Huang, Ping

doi:10.1080/00387010.2020.1759104

Cited by 3 publications

(4 citation statements)

References 49 publications

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“…Thus, a prediction model was developed basing on the chemometric analysis of these spectral variations. The model demonstrated 100% specificity and sensitivity in diagnosing heatstroke as a cause of death in rabbits [9].…”

Section: Postmortem Diagnosis Of Weather Related Deathmentioning

confidence: 97%

“…Furthermore, it has been reported that ATR-FTIR can be used to diagnose the death caused by hypothermia and heatstroke in rabbits basing on the analysis of rabbits' VH [9,10]. Concerning lethal hypothermia, chemometric analysis of spectral data generated from VH of hypothermic rabbits indicated a significant increase in the concentrations of glucose and nucleic acids.…”

Section: Postmortem Diagnosis Of Weather Related Deathmentioning

confidence: 99%

“…In addition, in certain homicide cases, biological evidences can help in predicting the cause of death and defining the postmortem interval. Therefore, sensitive detection and correct identification of biological evidences are essential for a successful outcome in criminal investigations [2,[5][6][7][8][9][10][11]. These tasks can only be achieved by adopting reliable and accurate analytical techniques and working protocols.…”

Section: Introductionmentioning

confidence: 99%

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Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses

Alkhuder

2022

Int J Legal Med

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Contemporary criminal investigations are based on the statements made by the victim and the eyewitnesses. They also rely on the physical evidences found in the crime scene. These evidences, and more particularly biological ones, have a great judicial value in the courtroom. They are usually used to revoke the suspect's allegations and confirm or refute the statements made by the victim and the witnesses. Stains of body fluids are biological evidences highly sought by forensic investigators. In many criminal cases, the success of the investigation relies on the correct identification and classification of these stains. Therefore, the adoption of reliable and accurate forensic analytical methods seems to be of vital importance to attain this objective. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) is a modern and universal analytical technique capable of fingerprint recognition of the analyte using minimal amount of the test sample. The current systematic review aims to through light on the fundamentals of this technique and to illustrate its wide range of applications in forensic investigations. ATR-FTIR is a nondestructive technique which has demonstrated an exceptional efficiency in detecting, identifying and discriminating between stains of various types of body fluids usually encountered in crime scenes. The ATR-FTIR spectral data generated from bloodstains can be used to deduce a wealth of information related to the donor species, age, gender, and race. These data can also be exploited to discriminate between stains of different types of bloods including menstrual and peripheral bloods. In addition, ATR-FTIR has a great utility in the postmortem investigations. More particularly, in estimating the postmortem interval and diagnosing death caused by extreme weather conditions. It is also useful in diagnosing some ambiguous death causes such as fatal anaphylactic shock and diabetic ketoacidosis.

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Section: Postmortem Diagnosis Of Weather Related Deathmentioning

confidence: 97%

Section: Postmortem Diagnosis Of Weather Related Deathmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses

Alkhuder

2022

Int J Legal Med

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“…Lin et al and Wu et al detected pulmonary edema fluid by FTIR imaging and constructed classification models for determining SCD, 41 anaphylactic shock, 42 hypothermia, 43 or diabetic ketoacidosis 44 as the cause. Tuo et al 45 and Wang et al 46 studied the vitreous fluid and liver tissue, respectively, of animal models of fatal hyperthermia and proposed FTIR spectroscopy as an alternative for determining the cause of death. These reports proved the application potential of FTIR spectroscopy in determining complex causes of death and inspired us to distinguish asphyxia from SCD as the cause of death.…”

Section: Introductionmentioning

confidence: 99%

Distinguishing Asphyxia from Sudden Cardiac Death as the Cause of Death from the Lung Tissues of Rats and Humans Using Fourier Transform Infrared Spectroscopy

et al. 2022

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The ability to determine asphyxia as a cause of death is important in forensic practice and helps us to judge whether a case is criminal. However, in some cases where the deceased has underlying heart disease, death by asphyxia cannot be determined by traditional autopsy and morphological observation under a microscope because there are no specific morphological features for either asphyxia or sudden cardiac death (SCD). Here, Fourier transform infrared (FTIR) spectroscopy was employed to distinguish asphyxia from SCD. A total of 40 lung tissues (collected at 0 h and 24 h postmortem) from 20 rats (10 died from asphyxia and 10 died from SCD) and 16 human lung tissues from 16 real cases were used for spectral data acquisition. After data preprocessing, 2675 spectra from rat lung tissues and 1526 spectra from human lung tissues were obtained for subsequent analysis. First, we found that there were biochemical differences in the rat lung tissues between the two causes of death by principal component analysis and partial least-squares discriminant analysis (PLS-DA), which were related to alterations in lipids, proteins, and nucleic acids. In addition, a PLS-DA classification model can be built to distinguish asphyxia from SCD. Second, based on the spectral data obtained from lung tissues allowed to decompose for 24 h, we could still distinguish asphyxia from SCD even when decomposition occurred in animal models. Nine important spectral features that contributed to the discrimination in the animal experiment were selected and further analyzed. Third, 7 of the 9 differential spectral features were also found to be significantly different in human lung tissues from 16 real cases. A support vector machine model was finally built by using the seven variables to distinguish asphyxia from SCD in the human samples. Compared with the linear PLS-DA model, its accuracy was significantly improved to 0.798, and the correct rate of determining the cause of death was 100%. This study shows the application potential of FTIR spectroscopy for exploring the subtle biochemical differences resulting from different death processes and determining the cause of death even after decomposition.

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Recent Chemometric Opportunities in Criminalistics

Thanni¹,

Sah²,

Harrington³

2021

Encyclopedia of Analytical Chemistry

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Recently, the growing applicability of chemometrics in various areas of civil and criminal investigations has been instrumental in resolving complex cases. As criminals become more inventive and measurement methods become more prevalent with onsite analysis and more sophisticated with increases in resolution and sensitivity, forensic analysts should be equipped with tools for advancing their laboratory analysis and investigations. The traditional mode of data analysis in criminalistics will not suffice for complex crime lab investigations, hence the need for the understanding and application of the statistical and chemometric tools is indispensable. Accurate interpretation of forensic evidence is critical for achieving justice. The use of chemometrics helps the analyst from designing effective and efficient experiments to the presentation of compelling evidence to the courts. This article highlights the most recent application of chemometrics in different forensic science areas and shows how pivotal statistical tools are to answering consequential questions in criminal investigations.

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Characterization and postmortem diagnosis of fatal heatstroke using Attenuated Total Reflectance Fourier transform infrared spectroscopy combined with chemometrics

Cited by 3 publications

References 49 publications

Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses

Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses

Distinguishing Asphyxia from Sudden Cardiac Death as the Cause of Death from the Lung Tissues of Rats and Humans Using Fourier Transform Infrared Spectroscopy

Recent Chemometric Opportunities in Criminalistics

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