Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models

Kovar, Joy L.; Volcheck, William; Sevick‐Muraca, Eva M.; Simpson, Melanie A.; Olive, D. Michael

doi:10.1016/j.ab.2008.09.050

Cited by 113 publications

(117 citation statements)

References 33 publications

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“…At this point, diffusion into the tumor is sometimes impeded by high hydrostatic pressure of many solid tumors, preventing homogeneous infiltration of the agent (1). Second, binding of the agent to specific epitopes of the cancer cell has to occur, and internalization of the agent can further amplify the fluorescence signal (23,40,41). Therefore, the cellular basement membrane is the third barrier, which can be passed using the transporter system of the cell by receptor-mediated endocytosis (33).…”

Section: What Is Required For a Target-specific Fluorescence Image?mentioning

confidence: 99%

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Keereweer

Driel

Snoeks

et al. 2013

Clinical Cancer Research

223

209

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Optical image-guided cancer surgery is a promising technique to adequately determine tumor margins by tumor-specific targeting, potentially resulting in complete resection of tumor tissue with improved survival. However, identification of the photons coming from the fluorescent contrast agent is complicated by autofluorescence, optical tissue properties, and accurate fluorescent targeting agents and imaging systems. All these factors have an important influence on the image that is presented to the surgeon. Considering the clinical consequences at stake, it is a prerequisite to answer the questions that are essential for the surgeon. What is optical image-guided surgery and how can it improve patient care? What should the oncologic surgeon know about the fundamental principles of optical imaging to understand which conclusions can be drawn from the images? And how do the limitations influence clinical decision making? This article discusses these questions and provides a clear overview of the basic principles and practical applications. Although there are limitations to the intrinsic capacity of the technique, when practical and technical surgical possibilities are considered, optical imaging can be a very powerful intraoperative tool in guiding the future oncologic surgeon toward radical resection and optimal clinical results.

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Section: What Is Required For a Target-specific Fluorescence Image?mentioning

confidence: 99%

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Keereweer

Driel

Snoeks

et al. 2013

Clinical Cancer Research

223

209

View full text Add to dashboard Cite

show abstract

“…The uptake of IRDye800-2DG in the liver was nonuniform and twice as high as in the normal tissue, which agrees with the elevated expression of GLUT1 transporters in liver tissue. 17 The role of GLUT1 transporters in uptaking IRDye800-2DG has been investigated by competitive binding assays and dose-dependence experiments in a variety of tumor cell lines. 17 Despite the reported role of GLUT1 transporters, 17 the exact uptake mechanism of IRDye800-2DG may be different from that of FDG or native glucose due to differences in molecular size.…”

Section: Tumor Glucose Metabolism Imagingmentioning

confidence: 99%

“…17 All experimental animal procedures were carried out in conformity with the guidelines of the US National Institutes of Health. The laboratory animal protocols for this work were approved by the Animal Studies Committee of Washington University in St. Louis.…”

Section: Animal Preparationmentioning

confidence: 99%

“…16 However, both PFI and FOT suffer from low spatial resolution and lack of intrinsic contrast, and have only been used to image tumor metabolism in superficial tumors. [16][17][18][19] In this paper, we use a ring-shaped confocal photoacoustic computed tomography imaging system (RC-PACT) 20 to image both anatomy and tumor glucose metabolism at high spatial resolution in a single imaging modality. The system is based on the photoacoustic effect, where absorbed laser energy in tissue generates a local temperature rise, which is subsequently converted to pressure via thermoelastic expansion.…”

Section: -3mentioning

confidence: 99%

“…21 Additionally, multiple absorbers whose absorption spectra are different can be spectrally separated by varying the laser wavelengths. 21 In RC-PACT, anatomical images were produced by endogenous hemoglobin contrast, and tumor glucose metabolism was imaged and quantified by spectrally separating the absorption of IRDye800-2DG 17 from that of hemoglobin. With the unique design of confocal free-space full-ring light illumination and full-ring ultrasound transducer array detection, our RC-PACT system generates in vivo cross-sectional images with simultaneous anatomical and metabolic assessments at high spatial resolution.…”

Section: -3mentioning

confidence: 99%

See 2 more Smart Citations

Tumor glucose metabolism imagedin vivoin small animals with whole-body photoacoustic computed tomography

et al. 2012

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Abstract. With the increasing use of small animals for human disease studies, small-animal whole-body molecular imaging plays an important role in biomedical research. Currently, none of the existing imaging modalities can provide both anatomical and glucose molecular information, leading to higher costs of building dual-modality systems. Even with image co-registration, the spatial resolution of the molecular imaging modality is not improved. Utilizing a ring-shaped confocal photoacoustic computed tomography system, we demonstrate, for the first time, that both anatomy and glucose uptake can be imaged in a single modality. Anatomy was imaged with the endogenous hemoglobin contrast, and glucose metabolism was imaged with a near-infrared dye-labeled 2-deoxyglucose.

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DNA‐Based Nanocarriers to Enhance the Optoacoustic Contrast of Tumors In Vivo

Joseph

Baumann

Postigo

et al. 2020

Adv Healthcare Materials

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Optoacoustic tomography (OT) enables non‐invasive deep tissue imaging of optical contrast at high spatio‐temporal resolution. The applications of OT in cancer imaging often rely on the use of molecular imaging contrast agents based on near‐infrared (NIR) dyes to enhance contrast at the tumor site. While these agents afford excellent biocompatibility and minimal toxicity, they present limited optoacoustic signal generation capability and rapid renal clearance, which can impede their tumor imaging efficacy. In this work, a synthetic strategy to overcome these limitations utilizing biodegradable DNA‐based nanocarrier (DNA‐NC) platforms is introduced. DNA‐NCs enable the incorporation of NIR dyes (in this case, IRDye 800CW) at precise positions to enable fluorescence quenching and maximize optoacoustic signal generation. Furthermore, these DNA‐NCs show a prolonged blood circulation compared to the native fluorophores, facilitating tumor accumulation by the enhanced permeability and retention (EPR) effect. In vivo imaging of tumor xenografts in mice following intravenous administration of DNA‐NCs reveals enhanced OT signals at 24 h when compared to free fluorophores, indicating promise for this method to enhance the optoacoustic signal generation capability and tumor uptake of clinically relevant NIR dyes.

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Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models

Cited by 113 publications

References 33 publications

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Tumor glucose metabolism imagedin vivoin small animals with whole-body photoacoustic computed tomography

DNA‐Based Nanocarriers to Enhance the Optoacoustic Contrast of Tumors In Vivo

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