Characterization and molecular cloning of a <i>serine hydroxymethyltransferase 1</i> (<i>OsSHM1</i>) in rice

Wang, Dekai; Liu, Heqin; Li, Sujuan; Zhai, Guowei; Shao, Jianfeng; Tao, Yuezhi

doi:10.1111/jipb.12336

Cited by 41 publications

(33 citation statements)

References 38 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, serine hydroxymethyltransferase (SHMT) is an essential component of photorespiration. A previous study found that an osshm1 mutant exhibited chlorotic lesions and a lethal phenotype ( Wang et al, 2015 ). Lin et al (2012) found that OsCATC could increase hydrogen peroxide in leaves, which consequently promoted NO production and led to cell death.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analyses of Gene Expression Changes Triggered by Rhizoctonia solani AG1 IA Infection in Resistant and Susceptible Rice Varieties

Zhang

Chen

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

Rice sheath blight, caused by Rhizoctonia solani, is one of the most devastating diseases for stable rice production in most rice-growing regions of the world. Currently, studies of the molecular mechanism of rice sheath blight resistance are scarce. Here, we used an RNA-seq approach to analyze the gene expression changes induced by the AG1 IA strain of R. solani in rice at 12, 24, 36, 48, and 72 h. By comparing the transcriptomes of TeQing (a moderately resistant cultivar) and Lemont (a susceptible cultivar) leaves, variable transcriptional responses under control and infection conditions were revealed. From these data, 4,802 differentially expressed genes (DEGs) were identified. Gene ontology and pathway enrichment analyses suggested that most DEGs and related metabolic pathways in both rice genotypes were common and spanned most biological activities after AG1 IA inoculation. The main difference between the resistant and susceptible plants was a difference in the timing of the response to AG1 IA infection. Photosynthesis, photorespiration, and jasmonic acid and phenylpropanoid metabolism play important roles in disease resistance, and the relative response of disease resistance-related pathways in TeQing leaves was more rapid than that of Lemont leaves at 12 h. Here, the transcription data include the most comprehensive list of genes and pathway candidates induced by AG1 IA that is available for rice and will serve as a resource for future studies into the molecular mechanisms of the responses of rice to AG1 IA.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analyses of Gene Expression Changes Triggered by Rhizoctonia solani AG1 IA Infection in Resistant and Susceptible Rice Varieties

Zhang

Chen

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The Arabidopsis mitochondrial SHMT is encoded by AtSHM1 and is required for the proper functioning of photorespiration (Somerville and Ogren, 1981;Voll et al, 2006). Its ortholog in rice (Oryza sativa) is OsSHM1 (Wang et al, 2015;Wu et al, 2015). AtSHM3 (Zhang et al, 2010) encodes an SHMT protein that is targeted to plastids, and AtSHM7 encodes an SHMT that is a nonfunctional enzyme and is found to be required for SAM biosynthesis in the nucleus and that epigenetically affects sulfur homeostasis .…”

Section: Discussionmentioning

confidence: 99%

Systematic Mutagenesis of Serine Hydroxymethyltransferase Reveals an Essential Role in Nematode Resistance

et al. 2017

View full text Add to dashboard Cite

Rhg4 is a major genetic locus that contributes to soybean cyst nematode (SCN) resistance in the Peking-type resistance of soybean (Glycine max), which also requires the rhg1 gene. By map-based cloning and functional genomic approaches, we previously showed that the Rhg4 gene encodes a predicted cytosolic serine hydroxymethyltransferase (GmSHMT08); however, the novel gain of function of GmSHMT08 in SCN resistance remains to be characterized. Using a forward genetic screen, we identified an allelic series of GmSHMT08 mutants that shed new light on the mechanistic aspects of GmSHMT08-mediated resistance. The new mutants provide compelling genetic evidence that Peking-type rhg1 resistance in cv Forrest is fully dependent on the GmSHMT08 gene and demonstrates that this resistance is mechanistically different from the PI 88788-type of resistance that only requires rhg1. We also demonstrated that rhg1-a from cv Forrest, although required, does not exert selection pressure on the nematode to shift from HG type 7, which further validates the bigenic nature of this resistance. Mapping of the identified mutations onto the SHMT structural model uncovered key residues for structural stability, ligand binding, enzyme activity, and protein interactions, suggesting that GmSHMT08 has additional functions aside from its main enzymatic role in SCN resistance. Lastly, we demonstrate the functionality of the GmSHMT08 SCN resistance gene in a transgenic soybean plant.

show abstract

“…Our previous studies indicated that overexpression of Lsi1 in chilling-sensitive Dular rice results in enhanced tolerance to low temperature; gene expression of OsSHMT was up-regulated in the Lsi1-OX rice in comparison with Dular rice [6]. Wang et al [13] found that OsSHMT is localised in the mitochondria, and it has also been reported to localise in the chloroplast, cytoplasm, nucleus, plasma membrane and cytosol [25,26]; despite these results, our present study indicated that OsSHMT is also localised in the endoplasmic reticulum, which is the main organelle for protein and lipid synthesis, membrane biogenesis, xenobiotic detoxification and cellular calcium storage [27]. [28].…”

Section: Discussionmentioning

confidence: 99%

Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance

Fang

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Rice is a temperature-sensitive crop that can sustain dramatic damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling. Results: A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit α, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit β. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit β, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment. Conclusions: Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit β is an intermediate junction between Lsi1 and OsSHMT.

show abstract

Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice

Cited by 41 publications

References 38 publications

Comparative Transcriptome Analyses of Gene Expression Changes Triggered by Rhizoctonia solani AG1 IA Infection in Resistant and Susceptible Rice Varieties

Comparative Transcriptome Analyses of Gene Expression Changes Triggered by Rhizoctonia solani AG1 IA Infection in Resistant and Susceptible Rice Varieties

Systematic Mutagenesis of Serine Hydroxymethyltransferase Reveals an Essential Role in Nematode Resistance

Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance

Contact Info

Product

Resources

About