Characterization and Identification of Proteolytic Bacteria From the Gut of the Velvetbean Caterpillar (Lepidoptera: Noctuidae)

Visôtto, Liliane Evangelista; Oliveira, M. G. A.; Ribon, A. O. B.; Mares-Guia, Thiago Rennó dos; Guedes, Raul Narciso C.

doi:10.1603/022.038.0415

Cited by 72 publications

(49 citation statements)

References 33 publications

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“…Diverse digestive enzymes have been identified from our annotation results. Studies on other lepidopterans also support that the isolated gut bacteria were able to hydrolyse starch, cellulose, pectin and protein …”

Section: Discussionmentioning

confidence: 86%

Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species

Chen

Xie

Zhang

et al. 2019

Pest Management Science

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BACKGROUND Many insect pests rely on microbial symbionts to obtain nutrients or for defence, thereby allowing them to exploit novel food sources and degrade environmental xenobiotics, including pesticides. Although Lepidoptera is one of the most diverse insect taxa and includes important agricultural pests, lepidopteran microbiotas, particularly functional traits, have not been studied widely. Here, we provide a comprehensive characterization of the gut microbiota across multiple mulberry‐feeding lepidopteran species, resolving both community structure and metabolic potential. RESULTS Our results indicate abundant bacteria inside the gut of larval Lepidoptera. However, even though they were fed the same diet, the structures of the bacterial communities differed in four major mulberry pest species, suggesting host‐specific effects on microbial associations. Community‐level metabolic reconstructions further showed that although taxonomic composition varied greatly, carbohydrate and amino acid metabolism and membrane transporter were key functional capabilities of the gut bacteria in all samples, which may play basic roles in the larval gut. In addition, principal coordinate analysis (PCoA) of gut bacterial‐predicted gene ontologies revealed specialized features of the microbiota associated with these mulberry pests, which were divided into two distinct clusters (macrolepidopterans and microlepidopterans). This pattern became even more prominent when further Lepidoptera species were involved. CONCLUSIONS A suite of gut microbiota metabolic functions significantly correlated with larval size; the metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism were specifically enriched in large species, while small larvae had enhanced nucleotide metabolism. Our report paves the way for uncovering the correlation between host phenotype and microbial symbiosis in this notorious insect pest group. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 86%

Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species

Chen

Xie

Zhang

et al. 2019

Pest Management Science

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“…In contrast to the results presented in the current study, Mika et al [20] did not isolate microorganisms with peptidase activity, but used an extract of the insects (e.g., Rhizoperta domenica; lesser grain borer). In the study of Visôtto et al [21], proteolytic microorganisms (substrate: casein) from the gut of the velvetbean caterpillar (Anticarsia gemmatalis) were screened. An optimization of the medium for B. subtilis 214 was performed to increase the extracellular peptidase activity (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Extracellular peptidases from insect- and compost-associated microorganisms: screening and usage for wheat gluten hydrolysis

Stressler

Eisele

Baur

et al. 2015

Eur Food Res Technol

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“…While the precise mode of cleavage has not been described, VBC larvae may produce a specific gut carboxypeptidase that is capable of cleaving the C-terminal Ala from the largely cyclic Vu-In. Thus far, trypsin-like proteases have been predominantly described; however, the gut tissues of A. gemmatalis larvae also harbor bacteria potentially capable of further proteolysis Visôtto et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

An Amino Acid Substitution Inhibits Specialist Herbivore Production of an Antagonist Effector and Recovers Insect-Induced Plant Defenses

et al. 2012

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Plants respond to insect herbivory through the production of biochemicals that function as either direct defenses or indirect defenses via the attraction of natural enemies. While attack by closely related insect pests can result in distinctive levels of induced plant defenses, precise biochemical mechanisms responsible for differing responses remain largely unknown. Cowpea (Vigna unguiculata) responds to Fall armyworm (Spodoptera frugiperda) herbivory through the detection of fragments of chloroplastic ATP synthase g-subunit proteins, termed inceptin-related peptides, present in larval oral secretions (OS). In contrast to generalists like Fall armyworm, OS of the legume-specializing velvetbean caterpillar (VBC; Anticarsia gemmatalis) do not elicit ethylene production and demonstrate significantly lower induced volatile emission in direct herbivory comparisons. Unlike all other Lepidoptera OS examined, which preferentially contain inceptin (Vu-In; + ICDINGVCVDA 2 ), VBC OS contain predominantly a C-terminal truncated peptide, Vu-In). Vu-In 2A is both inactive and functions as a potent naturally occurring antagonist of Vu-In-induced responses. To block antagonist production, amino acid substitutions at the C terminus were screened for differences in VBC gut proteolysis. A valine-substituted peptide (Vu-In+ ICDINGVCVDV 2 ) retaining full elicitor activity was found to accumulate in VBC OS. Compared with the native polypeptide, VBC that previously ingested 500 pmol of the valine-modified chloroplastic ATP synthase g-subunit precursor elicited significantly stronger plant responses in herbivory assays. We demonstrate that a specialist herbivore minimizes the activation of defenses by converting an elicitor into an antagonist effector and identify an amino acid substitution that recovers these induced plant defenses to a level observed with generalist herbivores.

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Characterization and Identification of Proteolytic Bacteria From the Gut of the Velvetbean Caterpillar (Lepidoptera: Noctuidae)

Cited by 72 publications

References 33 publications

Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species

Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species

Extracellular peptidases from insect- and compost-associated microorganisms: screening and usage for wheat gluten hydrolysis

An Amino Acid Substitution Inhibits Specialist Herbivore Production of an Antagonist Effector and Recovers Insect-Induced Plant Defenses

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