Characterization and expression of DDX6 during gametogenesis in the Chinese mitten crab Eriocheir sinensis

Abstract

ABSTRACT. DDX6 belongs to a family of DEAD-box RNA helicases, which are RNA splicing proteins that ensure the correct folding and structure of mature RNA. Gametogenesis requires the participation of many kinds of RNA. To explore its functions during Eriocheir sinensis gametogenesis, we cloned a full-length DDX6 cDNA sequence from E. sinensis (Es-DDX6) which contains a 1536-nucleotide open reading frame encoding a 512-amino acid protein. Multiple sequence alignments showed that Es-DDX6 has ten conservative DEAD-box family motifs. Tissue expression analysis of Es-DDX6 mRNA and protein levels showed that Es-DDX6 was highly expressed in both the ovary and testis. qRT-PCR analysis revealed the widespread expression of Es-DDX6 mRNA during various stages of gonad development peaking in October. In addition, immunohistochemical studies showed that oocytes and the spermatogonium and primary spermatocytes of testes contained high levels of cytoplasmic Es-DDX6 and decreased expression levels in spermatids. Interestingly, there was no expression of Es-DDX6 in these cells as they matured along the male reproductive system. Since oocytes and spermatocytes are active in meiosis and oocytes undergo 4421 DDX6 characterization and expression during gametogenesis ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4420-4437 (2015) rapid growth in October, these results provide preliminary evidence that Es-DDX6 plays a role in E. sinensis gametogenesis and oocyte growth processes.

De novo transcriptome sequencing and analysis of male and female swimming crab (Portunus trituberculatus) reproductive systems during mating embrace (stage II)

“…3 and 4 ). Gametogenesis is a complex process involving cellular transformations that result in the production of male and female haploid germ cells, and it starts from the mitotic cell cycle followed by entry into meiosis and the completion of complex differentiation programs [ 23 ]. Signal transduction is the process by which a chemical or physical signal is transmitted through a cell as a series of molecular events, and this process is also critical for the timing and maintenance of normal gametogenesis [ 24 ].…”

Genome-wide association study reveals genomic loci of sex differentiation and gonadal development in Plectropomus leopardus

“…It has also been proved that taf7l , as a paralogue of taf7 (transcription initiation factor TFIID subunit 7), cooperates with trf2 to regulate spermiogenesis ( Zhou et al, 2013 ), and the variation of taf7 affects the genetic etiology of idiopathic male infertility ( Pal et al, 2021 ). As reported, ddx6 (probable ATP-dependent RNA helicase DDX6) belongs to a family of DEAD-box RNA helicases that was highly expressed during various stages of gonad development in the Chinese mitten crab ( Eriocheir sinensis ) ( Li et al, 2015 ). Based on the ontogenic gonadal transcriptomes, the mechanisms of sex change of the ricefield eel ( Monopterus albus ) have been elucidated ( Fan et al, 2022 ), and the expression of apoeb (apolipoprotein Eb) detected was dramatically increased and peaked at the early intersexual stage.…”

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p>&nbsp;</p>

“…Usually in this taxon, genes of the nuage proteins are expressed only in the germ line. However, in the crab Eriocheir sinensis, esddx6 has low expression in some somatic tissues, such as heart, stomach, muscle, hemocytes, and cells of the thorax and intestine (Li et al 2015). Moreover, in the rhizocephalan cirripede Polyascus polygenea, ppvlg and ppdrh1 are expressed in cells of the stolons and buds of interna that will give rise to both the germline and the soma (Shukalyuk et al 2007).…”