Characterization and cytotoxicity of PLGA nanoparticles loaded with formononetin cyclodextrin complex

Guo, Bohong; Xu, Danqiao; Xiao-hong, Liu; Liao, Cancheng; Li, Shengbin; Huang, Zexian; Li, Xiaofang; Yi, Jun

doi:10.1016/j.jddst.2017.08.010

Cited by 20 publications

(8 citation statements)

References 19 publications

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“…Drug delivery systems are strategies employed to overcome the low bioavailability and low water solubility of formononetin to achieve its pharmacological efficacy at minimum dose. A number of efforts have had promising results in developing efficient drug carriers to deliver formononetin to the target site, including the hydroxypropyl-β-cyclodextrin-modified carboxylated single-walled carbon nanotubes (CD-SWCNTs) (Liu et al, 2018), multiwalled carbon nanotube (Guo et al, 2018), poly(lactic- co -glycolic acid) (PLGA)-nanoparticle loaded with formononetin hydroxypropyl-β-cyclodextrin complex (Guo et al, 2017a), and D -α-tocopheryl polyethylene glycol 1000 succinate (TPGS) micelles containing formononetin (Cheng et al, 2016). These drug delivery methods have successfully improved the solubility and absorption of formononetin.…”

Section: Potential Drug Delivery Of Formononetinmentioning

confidence: 99%

“…Yet another intervention was developed by incorporating formononetin in 2-hydroxypropyl-β-cyclodextrin inclusion complex loaded in PLGA-nanoparticles with a size of ∼200 nm. The intervention was able to exert a sustained release effect with cumulative release of 50% of formononetin over 24 h. However, the cytotoxicity of this intervention was slightly weaker as compared to free formononetin, and this was suggested to be due to incomplete release of formononetin from the intervention (Guo et al, 2017a).…”

Section: Potential Drug Delivery Of Formononetinmentioning

confidence: 99%

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Formononetin: A Review of Its Anticancer Potentials and Mechanisms

et al. 2019

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Cancer, a complex yet common disease, is caused by uncontrolled cell division and abnormal cell growth due to a variety of gene mutations. Seeking effective treatments for cancer is a major research focus, as the incidence of cancer is on the rise and drug resistance to existing anti-cancer drugs is major concern. Natural products have the potential to yield unique molecules and combinations of substances that may be effective against cancer with relatively low toxicity/better side effect profile compared to standard anticancer therapy. Drug discovery work with natural products has demonstrated that natural compounds display a wide range of biological activities correlating to anticancer effects. In this review, we discuss formononetin (C 16 H 12 O 4 ), which originates mainly from red clovers and the Chinese herb Astragalus membranaceus . The compound comes from a class of 7-hydroisoflavones with a substitution of methoxy group at position 4. Formononetin elicits antitumorigenic properties in vitro and in vivo by modulating numerous signaling pathways to induce cell apoptosis (by intrinsic pathway involving Bax, Bcl-2, and caspase-3 proteins) and cell cycle arrest (by regulating mediators like cyclin A, cyclin B1, and cyclin D1), suppress cell proliferation [by signal transducer and activator of transcription (STAT) activation, phosphatidylinositol 3-kinase/protein kinase-B (PI3K/AKT), and mitogen-activated protein kinase (MAPK) signaling pathway], and inhibit cell invasion [by regulating growth factors vascular endothelial growth factor (VEGF) and Fibroblast growth factor 2 (FGF2), and matrix metalloproteinase (MMP)-2 and MMP-9 proteins]. Co-treatment with other chemotherapy drugs such as bortezomib, LY2940002, U0126, sunitinib, epirubicin, doxorubicin, temozolomide, and metformin enhances the anticancer potential of both formononetin and the respective drugs through synergistic effect. Compiling the evidence thus far highlights the potential of formononetin to be a promising candidate for chemoprevention and chemotherapy.

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Section: Potential Drug Delivery Of Formononetinmentioning

confidence: 99%

Section: Potential Drug Delivery Of Formononetinmentioning

confidence: 99%

Formononetin: A Review of Its Anticancer Potentials and Mechanisms

et al. 2019

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“…The PLGA was selected in our research as a sustained release polymer due to its biodegradability and biocompatibility nature [35]. Solvent shifting method is based on the interfacial deposition of a polymer following shifting of a semi-polar solvent (Acetone in our research) miscible with water from a lipophilic solution.…”

Section: Discussionmentioning

confidence: 99%

QbD enabled optimization of solvent shifting method for fabrication of PLGA-based nanoparticles for promising delivery of Capecitabine for antitumor activity

Jena

Patra

Panigrahi

et al. 2021

Drug Deliv. and Transl. Res.

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The key objective of the current research was to fabricate and optimize Capecitabine loaded PLGA based nanoparticles (NPs) by enabling Quality by Design (QbD) approach for enhancing antitumor activity by promising delivery of the drug at the colonic site. The current research was based on fabricating PLGA based nanoparticles along with eudragit S100 as enteric polymer employing solvent shifting method followed by optimization using QbD approach. This approach was found to be useful for understanding the multiple factors and their interaction influencing the product by utilizing Design of Experiment (DOE). Box-Behken Design (BBD) was adopted to achieve the required critical quality attributes (CQAs) i.e. minimizing particle size, maximizing entrapment efficiency and minimizing PDI value. The optimized nanoparticles were lyophilized and characterized by FT-IR, DSC, TEM, DLS, MTT assay using HT-29 cell lines and in vivo pharmacokinetic studies. The optimized PLGA based nanoparticles were found to possess average particle size, PDI, zeta potential as well as entrapment efficiency of 195 nm, 0.214, -6.65 mV and 65% respectively. TEM analysis revealed the spherical nature of nanoparticles. The FT-IR and DSC studies revealed no interaction. The bioavailability of Capecitabine loaded nanoparticles was found to be two fold increased than the pure drug and also it exhibited significantly more cytotoxic to tumor cells as compared to pure drug as confirmed by MTT assay using HT 29 cell lines. The optimized PLGA based nanoparticles found to possess enhanced bioavailability and significantly more cytotoxic potential i.e. antitumor activity as compared to pure drug.

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“…However, some recent studies have indicated that even the biodegradable polymer, such as, PLGA, can demonstrate toxicological profile. [ 143 , 144 , 145 ] This is because the physicochemical properties of the polymers are susceptible to alteration in their nanoformulations due to high surface area to mass ratios of nanostructures. [ 146 ] Xion et al have demonstrated how the size influenced cytotoxicity in both RAW264.7 cells and BEAS‐2B cells.…”

Section: Significance Of Polymers In Nanocarrier Designmentioning

confidence: 99%

Structure‐Based Varieties of Polymeric Nanocarriers and Influences of Their Physicochemical Properties on Drug Delivery Profiles

2022

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Carriers are equally important as drugs. They can substantially improve bioavailability of cargos and safeguard healthy cells from toxic effects of certain therapeutics. Recently, polymeric nanocarriers (PNCs) have achieved significant success in delivering drugs not only to cells but also to subcellular organelles. Variety of natural sources, availability of different synthetic routes, versatile molecular architectures, exploitable physicochemical properties, biocompatibility, and biodegradability have presented polymers as one of the most desired materials for nanocarrier design. Recent innovative concepts and advances in PNC-associated nanotechnology are providing unprecedented opportunities to engineer nanocarriers and their functions. The efficiency of therapeutic loading has got considerably increased. Structural design-based varieties of PNCs are widely employed for the delivery of small therapeutic molecules to genes, and proteins. PNCs have gained ever-increasing attention and certainly paves the way to develop advanced nanomedicines. This article presents a comprehensive investigation of structural design-based varieties of PNCs and the influences of their physicochemical properties on drug delivery profiles with perspectives highlighting the inevitability of incorporating both the multi-stimuli-responsive and multi-drug delivery properties in a single carrier to design intelligent PNCs as new and emerging research directions in this rapidly developing area.

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Characterization and cytotoxicity of PLGA nanoparticles loaded with formononetin cyclodextrin complex

Cited by 20 publications

References 19 publications

Formononetin: A Review of Its Anticancer Potentials and Mechanisms

Formononetin: A Review of Its Anticancer Potentials and Mechanisms

QbD enabled optimization of solvent shifting method for fabrication of PLGA-based nanoparticles for promising delivery of Capecitabine for antitumor activity

Structure‐Based Varieties of Polymeric Nanocarriers and Influences of Their Physicochemical Properties on Drug Delivery Profiles

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