Characterization and comparative assessment of antioxidant and ACE inhibitory activities of thornback ray gelatin hydrolysates

Peptides: Production, bioactivity, functionality, and applications

“…This is because it affects size, amount, amino acid composition and amino acid sequence of the peptides produced, which in turn influences the bioactive and functional properties of the hydrolysates (Sarmadi and Ismail, 2010). Although crude proteases extracts (e.g., from fish) have been successfully used as catalysts in enzymatic hydrolysis Lassoued et al, 2015b), commercially purified enzymes are preferably employed since they allow a better control over the hydrolysis process (e.g., shorter reaction time for a desired DH, more consistent peptides size and composition) (Samaranayaka and Li-Chan, 2011). Consequently, industrial proteases derived from different sources such as microorganisms (e.g., Alcalase, Neutrase, Protease P "Amano" 6, Flavourzyme, Protamex) (Halldorsdottir et al, 2013;Venuste et al, 2013), animals (e.g., PTN, pepsin, trypsin, a-chymotrypsin, pancreatin) (Wu et al, 2015a, b;Garc ıa-Moreno et al, 2017) and plants (e.g., papain, bromelain) (Salampessy et al, 2015;Elavarasan et al, 2016) have been widely employed for the production of protein hydrolysates exhibiting bioactive and/or functional properties.…”

Angiotensin Converting Enzyme Inhibition with Antiradicalar Activity of Thornback Ray Gelatin Hydrolysate: Determination and Separation of Peptide Fractions

“…The activity increased 7.5-fold, suggesting that ACE inhibitory peptides are encrypted within the sequence of the parent protein. High ACE inhibitory activity of TRGH-Alcalase was attributed to the high level of hydrophobic peptides [19]. Byun, et al [15] reported also the ACE inhibitory activity of Alaska pollack skin digested by Alcalase; the IC 50 value is 0.629 mg/ml.…”

“…Thornback Ray Gelatin Hydrolysate (TRGH) generated by Alcalase, was prepared according to Lassoued, et al [19]. The degree of hydrolysis, defined as the percent ratio of the number of peptide bonds broken to the total number of peptide bonds per unit weight (h tot ), in each case, was calculated from the amount of base (NaOH) added to keep the pH constant during the hydrolysis [22].…”

“…However, the IC 50 value of TRGH-Alcalase is higher than 5 mg/ml. This low activity was attributed to the low content of amino acids that enhance the scavenging activities of peptides (Trp, Tyr and Met) [19]. Where does the interest of hydrolysate fractionation in order to get a concentrate of antioxidant peptides.…”

“…In a previous study, Thornback Ray Skin's Gelatin Hydrolysate obtained after treatment with Alcalase (TRGH-Alcalase), was characterized in terms of hydrophobicity, physico-chemical properties, and amino acid composition, as well as the comparative assessment of its antioxidant and ACE inhibitory capacities [19]. However, there is no information on the relationships between peptide physico-chemical characteristics and potential bioactive properties.…”