Characterization and cellular uptake of platinum anticancer drugs encapsulated in apoferritin

Xing, Ruimin; Wang, Xiaoyong; Zhang, Changli; Zhang, Yangmiao; Wang, Qi; Yang, Zhen; Guo, Zijian

doi:10.1016/j.jinorgbio.2009.05.001

Cited by 115 publications

(90 citation statements)

References 36 publications

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“…Thus, we assume that the commonly used medical radioisotopes such as gallium-67, rubidium-82, copper-64, technetium-99m, indium-111, xenon-133, thallium-201, and yttrium-90 can also be loaded into HFn nanocages for tumor-targeted therapy and diagnosis. So far, a great number of foreign molecules have been successfully encapsulated into ferritin nanocages (18)(19)(20)(21)(22)(23)(24), indicating that HFn is a universal drug carrier for tumor-targeted delivery.…”

Section: Easy Scaling-up and Manufacturing With Robust And Reproduciblementioning

confidence: 99%

“…In the present study, we loaded HFn nanocage with doxorubicin (Dox) for tumor-specific drug delivery. HFn nanocages can encapsulate large amounts of foreign molecules (18)(19)(20)(21)(22)(23)(24), bind specifically to tumor cells that overexpress TfR1 (17), and should be able to efficiently deliver high doses of therapeutic drugs to tumors. In particular, natural HFn nanocarriers are expected to possess an outstanding biocompatibility and safety profile, because they exist naturally in the human body and are composed of nontoxic elements that therefore would not activate inflammatory or immunological responses (25).…”

mentioning

confidence: 99%

“…In addition, many currently available methods for drug loading into ferritin involves disassembling ferritin nanocages in severe acidic pH (18)(19)(20)(21)(22), which irreversibly damages ferritin protein cages and forms hole defects on the spherical protein surface (30). The irreversible damages to ferritin will seriously affect their in vivo stability and drug delivery efficiency.…”

mentioning

confidence: 99%

“…Compared with the clinically approved liposomal Dox (Doxil), HFn-Dox exhibited longer median survival times and lower toxicity when administered at the same dose in all tumor models studied. the published work on ferritin-based drug delivery only reported in vitro results (18)(19)(20)(21), reflecting that the drug-loaded ferritin prepared using the acidic pH method might not be suitable for in vivo applications.…”

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confidence: 99%

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H-ferritin–nanocaged doxorubicin nanoparticles specifically target and kill tumors with a single-dose injection

Liang

Fan

Zhou

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

412

438

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An ideal nanocarrier for efficient drug delivery must be able to target specific cells and carry high doses of therapeutic drugs and should also exhibit optimized physicochemical properties and biocompatibility. However, it is a tremendous challenge to engineer all of the above characteristics into a single carrier particle. Here, we show that natural H-ferritin (HFn) nanocages can carry high doses of doxorubicin (Dox) for tumor-specific targeting and killing without any targeting ligand functionalization or property modulation. Doxloaded HFn (HFn-Dox) specifically bound and subsequently internalized into tumor cells via interaction with overexpressed transferrin receptor 1 and released Dox in the lysosomes. In vivo in the mouse, HFn-Dox exhibited more than 10-fold higher intratumoral drug concentration than free Dox and significantly inhibited tumor growth after a single-dose injection. Importantly, HFn-Dox displayed an excellent safety profile that significantly reduced healthy organ drug exposure and improved the maximum tolerated dose by fourfold compared with free Dox. Moreover, because the HFn nanocarrier has well-defined morphology and does not need any ligand modification or property modulation it can be easily produced with high purity and yield, which are requirements for drugs used in clinical trials. Thus, these unique properties make the HFn nanocage an ideal vehicle for efficient anticancer drug delivery.

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Section: Easy Scaling-up and Manufacturing With Robust And Reproduciblementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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H-ferritin–nanocaged doxorubicin nanoparticles specifically target and kill tumors with a single-dose injection

Liang

Fan

Zhou

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

412

438

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“…The spherical cage in apoferritin can be used for the encapsulation of fluorescent dyes [77], drugs [78][79][80][81][82][83][84][85], magnetic resonance imaging contrast agents [86,87], genes [88] or other compounds [89]. Due to the self-assembly activity of apoferritin and its response to the surrounding environment, it has several advantages for use as a nanocarrier.…”

Section: Apoferritin As a Drug / Gene Nanocarriermentioning

confidence: 99%