Characterization and cDNA sequence of Bothriechis schlegelii l-amino acid oxidase with antibacterial activity

Muñoz, Leidy Johana Vargas; Estrada-Gómez, Sebastián; Núñez, Vitelbina; Sanz, Libia; Calvete, Juan J.

doi:10.1016/j.ijbiomac.2014.05.039

Cited by 15 publications

(11 citation statements)

References 36 publications

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“…Interestingly, BsLAAO from the venom of Bothriechis schlegelii has selective antibacterial activities against S. aureus with a MIC of 4 μg/mL and A. baumannii with a MIC of 2 μg/mL. In contrast, E. coli was found to be resistant to BsLAAO (Vargas Muñoz et al 2014). In particular, the LAAO isolated from the venom of the king cobra Ophiophagus hannah (OH-LAAO) has the most effective inhibitory effect against two Staphylococci (S. aureus and S. epidermidis), with MIC values of 0.78 and 1.56 μg/mL, respectively .…”

Section: Selective Antibacterial Actions Of Laaosmentioning

confidence: 99%

“…Furthermore, bacteria treated with several LAAOs changed the morphology of the target cell (Vargas et al 2013;Vargas Muñoz et al 2014;Wang et al 2011;Ehara et al 2002;Kitani et al 2008;Kasai et al 2015) and membrane permeability (Yang et al 2011). The LAAOs-treated bacteria damaged the cell surface and formed surface aggregates, and the cells had a deformed appearance.…”

Section: Platichthys-stellatus--------------505--tsiysnlldvstqtrdef--mentioning

confidence: 99%

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Antibacterial properties of l-amino acid oxidase: mechanisms of action and perspectives for therapeutic applications

Kasai

Ishikawa

Nakamura

et al. 2015

Appl Microbiol Biotechnol

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Venom, the mucus layer covering the body surface, ink glands, mammary glands, milk, and various animal secretory functions as both a physical and chemical defense barrier against bacteria and virus infections. Previously, several studies reported that L-amino acid oxidases (LAAOs) present in animal secretary fluids have strong antimicrobial activities and selective cytotoxic activities against Gram-positive and Gram-negative bacteria, various pathogenic bacteria, viruses, and parasite species. These LAAOs catalyze oxidative deamination of an L-amino acid substrate with the generation of hydrogen peroxide. The antibacterial activity of LAAOs is completely inhibited by catalase; thus, LAAOs kill bacteria by the hydrogen peroxide generated from the oxidation of L-amino acid substrates. This review focuses on the selective, specific, and local antibacterial actions of various LAAOs that may be used as novel therapeutic agents against infectious diseases. LAAOs that are suitable leads for combating multidrug-resistant bacterial infections are also studied.

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Section: Selective Antibacterial Actions Of Laaosmentioning

confidence: 99%

Section: Platichthys-stellatus--------------505--tsiysnlldvstqtrdef--mentioning

confidence: 99%

Antibacterial properties of l-amino acid oxidase: mechanisms of action and perspectives for therapeutic applications

Kasai

Ishikawa

Nakamura

et al. 2015

Appl Microbiol Biotechnol

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“…LAAOs play important roles in the biological activities for snake venom (SV). SV-LAAOs induce platelet aggregation and cell apoptosis, exhibit anti-microbial, anti-leishmaniasis, anti-tumor and anti-HIV activities 7 8 9 10 11 12 13 14 15 . SV-LAAOs were reported to exhibit cytotoxicity and apoptosis-induction towards leukemia HL-60 17 19 and K562 cells 20 , gastric carcinoma AGS and breast carcinoma MCF-7 cells 21 , colorectal carcinoma RKO cells 14 , melanoma B16 cells 17 , cervical carcinoma Hela cells 20 and lung carcinoma A549 cells 22 .…”

Section: Discussionmentioning

confidence: 99%

“…As one major snake venom (SV) component, LAAO commonly exists as homodimeric FAD-(flavin adenine dinucleotide) or FMN-(flavin mono-nucleotide) glycoprotein 4 5 6 . The anti-microbial, anti-platelet and anti-tumor functions 7 8 9 10 11 12 13 14 15 of SV-LAAOs were commonly reported to be mediated by enzymatic- released H 2 O 2 16 17 18 . However, the underlying action mechanisms are still unclear.…”

mentioning

confidence: 99%

Akbu-LAAO exhibits potent anti-tumor activity to HepG2 cells partially through produced H2O2 via TGF-β signal pathway

Guo

Liu

Dong

et al. 2015

Sci Rep

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Previously, we characterized the biological properties of Akbu-LAAO, a novel L-amino acid oxidase from Agkistrodon blomhoffii ussurensis snake venom (SV). Current work investigated its in vitro anti-tumor activity and underlying mechanism on HepG2 cells. Akbu-LAAO inhibited HepG2 growth time and dose-dependently with an IC50 of ~38.82 μg/mL. It could induce the apoptosis of HepG2 cells. Akbu-LAAO exhibited cytotoxicity by inhibiting growth and inducing apoptosis of HepG2 as it showed no effect on its cell cycle. The inhibition of Akbu-LAAO to HepG2 growth partially relied on enzymatic-released H2O2 as catalase only partially antagonized this effect. cDNA microarray results indicated TGF-β signaling pathway was linked to the cytotoxicity of Akbu-LAAO on HepG2. TGF-β pathway related molecules CYR61, p53, GDF15, TOB1, BTG2, BMP2, BMP6, SMAD9, JUN, JUNB, LOX, CCND1, CDK6, GADD45A, CDKN1A were deregulated in HepG2 following Akbu-LAAO stimulation. The presence of catalase only slightly restored the mRNA changes induced by Akbu-LAAO for differentially expressed genes. Meanwhile, LDN-193189, a TGF-β pathway inhibitor reduced Akbu-LAAO cytotoxicity on HepG2. Collectively, we reported, for the first time, SV-LAAO showed anti-tumor cell activity via TGF-β pathway. It provides new insight of SV-LAAO exhibiting anti-tumor effect via a novel signaling pathway.

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“…The antibacterial activity of svLAAOs was reported more than forty years ago ( Skarnes, 1970 ) in Crotalus adamanteus venom. Since then, other authors have also reported antibacterial activity against Gram-positive and Gram-negative bacteria ( Toyama et al, 2006 ; Stábeli et al, 2007 ; Rodrigues et al, 2009 ; Zhong et al, 2009 ; Lee et al, 2011 ; Vargas et al, 2013 ; Vargas Muñoz et al, 2014 ; Lazo et al, 2017 ). However, such activity has not been reported for a Micrurus mipartitus venom enzyme.…”

Section: Introductionmentioning

confidence: 99%

MipLAAO, a new L-amino acid oxidase from the redtail coral snake Micrurus mipartitus

Rey-Suárez

Acosta

Torres

et al. 2018

PeerJ

Self Cite

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L-amino acid oxidases (LAAOs) are ubiquitous enzymes in nature. Bioactivities described for these enzymes include apoptosis induction, edema formation, induction or inhibition of platelet aggregation, as well as antiviral, antiparasite, and antibacterial actions. With over 80 species, Micrurus snakes are the representatives of the Elapidae family in the New World. Although LAAOs in Micrurus venoms have been predicted by venom gland transcriptomic studies and detected in proteomic studies, no enzymes of this kind have been previously purified from their venoms. Earlier proteomic studies revealed that the venom of M. mipartitus from Colombia contains ∼4% of LAAO. This enzyme, here named MipLAAO, was isolated and biochemically and functionally characterized. The enzyme is found in monomeric form, with an isotope-averaged molecular mass of 59,100.6 Da, as determined by MALDI-TOF. Its oxidase activity shows substrate preference for hydrophobic amino acids, being optimal at pH 8.0. By nucleotide sequencing of venom gland cDNA of mRNA transcripts obtained from a single snake, six isoforms of MipLAAO with minor variations among them were retrieved. The deduced sequences present a mature chain of 483 amino acids, with a predicted pI of 8.9, and theoretical masses between 55,010.9 and 55,121.0 Da. The difference with experimentally observed mass is likely due to glycosylation, in agreement with the finding of three putative N-glycosylation sites in its amino acid sequence. A phylogenetic analysis of MmipLAAO placed this new enzyme within the clade of homologous proteins from elapid snakes, characterized by the conserved Serine at position 223, in contrast to LAAOs from viperids. MmipLAAO showed a potent bactericidal effect on S. aureus (MIC: 2 µg/mL), but not on E. coli. The former activity could be of interest to future studies assessing its potential as antimicrobial agent.

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Characterization and cDNA sequence of Bothriechis schlegelii l-amino acid oxidase with antibacterial activity

Cited by 15 publications

References 36 publications

Antibacterial properties of l-amino acid oxidase: mechanisms of action and perspectives for therapeutic applications

Antibacterial properties of l-amino acid oxidase: mechanisms of action and perspectives for therapeutic applications

Akbu-LAAO exhibits potent anti-tumor activity to HepG2 cells partially through produced H2O2 via TGF-β signal pathway

MipLAAO, a new L-amino acid oxidase from the redtail coral snake Micrurus mipartitus

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