Characterization and assessment of hyperelastic and elastic properties of decellularized human adipose tissues

Omidi, Ehsan; Fuetterer, Lydia; Mousavi, Seyed Reza; Armstrong, Ryan C.; Flynn, Lauren E.; Samani, Abbas

doi:10.1016/j.jbiomech.2014.09.035

Cited by 61 publications

(54 citation statements)

References 22 publications

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“…The 5 µm spherical AFM tip enabled indentation testing over a larger region of the coatings, providing an analysis of the properties on the scale of individual cells. Notably, the α‐amylase‐digested DAT coatings were stiffer than the previous values measured through bulk compression testing for the intact DAT and α‐amylase‐digested DAT foams, which were in the range of ≈2–4 kPa and similar to the values reported for native human fat . The increased stiffness of the coatings may be related to the difference in the scale of the testing methods employed, as well as structural differences in the materials and their processing to form coatings.…”

Section: Discussionmentioning

confidence: 46%

Culture on Tissue‐Specific Coatings Derived from α‐Amylase‐Digested Decellularized Adipose Tissue Enhances the Proliferation and Adipogenic Differentiation of Human Adipose‐Derived Stromal Cells

Shridhar

Lam

Sun

et al. 2019

Biotechnology Journal

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While extracellular matrix (ECM)‐derived coatings have the potential to direct the response of cell populations in culture, there is a need to investigate the effects of ECM sourcing and processing on substrate bioactivity. To develop improved cell culture models for studying adipogenesis, the current study examines the proliferation and adipogenic differentiation of human adipose‐derived stem/stromal cells (ASCs) on a range of ECM‐derived coatings. Human decellularized adipose tissue (DAT) and commercially available bovine tendon collagen (COL) are digested with α‐amylase or pepsin to prepare the coatings. Physical characterization demonstrates that α‐amylase digestion generates softer, thicker, and more stable coatings, with a fibrous tissue‐like ultrastructure that is lost in the pepsin‐digested thin films. ASCs cultured on the α‐amylase‐digested ECM have a more spindle‐shaped morphology, and proliferation is significantly enhanced on the α‐amylase‐digested DAT coatings. Further, the α‐amylase‐digested DAT provides a more pro‐adipogenic microenvironment, based on higher levels of adipogenic gene expression, glycerol‐3‐phosphate dehydrogenase (GPDH) enzyme activity, and perilipin staining. Overall, this study supports α‐amylase digestion as a new approach for generating bioactive ECM‐derived coatings, and demonstrates tissue‐specific bioactivity using adipose‐derived ECM to enhance ASC proliferation and adipogenic differentiation.

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Section: Discussionmentioning

confidence: 46%

Culture on Tissue‐Specific Coatings Derived from α‐Amylase‐Digested Decellularized Adipose Tissue Enhances the Proliferation and Adipogenic Differentiation of Human Adipose‐Derived Stromal Cells

Shridhar

Lam

Sun

et al. 2019

Biotechnology Journal

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“…The methodology for decellularizing adipose tissue was pioneered by Flynn and colleagues [ 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ] and has since been confirmed by multiple independent laboratories [ 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 ]. These methods combine biological (enzyme digestion), chemical, and physical processing steps to achieve the uniform and consistent manufacture of protein scaffolds depleted of contaminating genomic DNA and lipids.…”

Section: Extracellular Matrix (Ecm) From Decellularized Adipose Timentioning

confidence: 99%

Clinical Translational Potential in Skin Wound Regeneration for Adipose-Derived, Blood-Derived, and Cellulose Materials: Cells, Exosomes, and Hydrogels

Frazier¹,

Alarcon²,

et al. 2020

Biomolecules

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Acute and chronic skin wounds due to burns, pressure injuries, and trauma represent a substantial challenge to healthcare delivery with particular impacts on geriatric, paraplegic, and quadriplegic demographics worldwide. Nevertheless, the current standard of care relies extensively on preventive measures to mitigate pressure injury, surgical debridement, skin flap procedures, and negative pressure wound vacuum measures. This article highlights the potential of adipose-, blood-, and cellulose-derived products (cells, decellularized matrices and scaffolds, and exosome and secretome factors) as a means to address this unmet medical need. The current status of this research area is evaluated and discussed in the context of promising avenues for future discovery.

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“…Through the analysis of published decellularization methods, the physical steps included repeated freeze‐thaw, homogenization, mechanical massaging, stirring or high‐pressure treatment. Among them, three cycles of freeze‐thaw, from −80°C to 37°C, was first used by Flynn in the process of adipose decellularization, which was the most widely adopted method by researchers . This technique could minimize the amounts of chemical agents required for effective decellularization.…”

Section: Preparation Of Datmentioning

confidence: 99%

Recent developments and clinical potential on decellularized adipose tissue

Dong

Zhang

et al. 2018

J Biomedical Materials Res

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Natural and synthetic materials have been developed to provide a three-dimensional (3-D) scaffold that mimics the extracellular matrix properties for tissue regeneration and reconstruction. On account of the excellent biological and mechanical properties, diverse decellularized tissue materials have been developed as natural scaffolds. Among them, decellularized adipose tissue (DAT) has generated great interest in recent years due to the extensiveness of the source and the eximious potential to enhance the regeneration and repair of damaged tissues. In this review, we compared the different physical, chemical, and biological treatment in recorded decellularization methods, and illustrated the similarities and differences in the characteristics of various aspects of DATs prepared by different methods, including macroscopic (macro) and microcosmic (micro) structure, cellular contents removal, DNA quantification, collagen, glycosaminoglycan (GAG) or growth factors retention, and mechanical properties. We also summarized the DAT application in the studies of tissue regeneration, wound healing, drug screening, and other diseases. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2562-2573, 2018.

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Characterization and assessment of hyperelastic and elastic properties of decellularized human adipose tissues

Cited by 61 publications

References 22 publications

Culture on Tissue‐Specific Coatings Derived from α‐Amylase‐Digested Decellularized Adipose Tissue Enhances the Proliferation and Adipogenic Differentiation of Human Adipose‐Derived Stromal Cells

Culture on Tissue‐Specific Coatings Derived from α‐Amylase‐Digested Decellularized Adipose Tissue Enhances the Proliferation and Adipogenic Differentiation of Human Adipose‐Derived Stromal Cells

Clinical Translational Potential in Skin Wound Regeneration for Adipose-Derived, Blood-Derived, and Cellulose Materials: Cells, Exosomes, and Hydrogels

Recent developments and clinical potential on decellularized adipose tissue

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