Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.11.044 View full text
Róbert Góra, Milan Hutta, Pavol Rohárik

Abstract: Liquid chromatography method arranged around RP-HPLC using step-wise gradients of dimethylformamide (DMF) in pH 3.0 aqueous phosphate buffered mobile phase and a wide-pore (30 nm) 4mm inner diameter (I.D.) octadecylsilica column was applied to the fractionation separation of soil and peat humic substances (HS), respectively. Combination of acceptable DMF solvating properties for HSs together with wide pore RP sorbent improved surface interactions of the analytes and suppressed influence of size-exclusion effec…

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