Acta Biotechnol. volume 18, issue 4, P339-351 1998 DOI: 10.1002/abio.370180408 View full text
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V. Ivanova, E. Dobreva, M. D. Legoy

Abstract: Covalent immobilization of thermostable a-amyl& from catabolite resistant and sensitive Bacillus lichenifom's strains on controlled pore glass (CPG) and porous silica (Spherosil) beads and ionic binding on DEAE-cellulose. Amberlite and Dowex were investigated. Preparations with satisfactory operational stabilities and activities up to 1.600 U/g of support (ionic binding) and 800 U/g carrier (covalent coupling) were obtained. Immobilization led to a narrowing of the pH interval of maximum activity. The fured a…

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