Characteristics of complexes between poly(propylene imine) dendrimers and nucleotides

Szulc, Agata; Appelhans, Dietmar; Voit, Brigitte; Bryszewska, Maria; Klajnert, Barbara

doi:10.1039/c2nj40165g

Cited by 13 publications

(28 citation statements)

References 34 publications

(68 reference statements)

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“…Our scientific interests have concentrated on the possibility of using so‐called open‐shell (OS) poly(propyleneimine) (PPI) dendrimers with their surfaces partially coated with maltose units for the intracellular delivery of active triphosphate forms of adenosine analogues. Such glycodendrimers exhibit high biocompatibility as well as the capacity to form stable complexes with nucleoside triphosphates and to protect them from enzymatic degradation . We proved that the fourth generation maltose‐modified PPI dendrimer (PPI‐Mal OS G4) can serve as an efficient nanocarrier for an active metabolite of fludarabine (Ara‐FATP), increasing its cytotoxic activity and overcoming NT‐associated resistance due to the independent mechanism of cell entry …”

Section: Zeta Potential Of Ppi Dendrimers and Nucleotide–dendrimer Comentioning

confidence: 98%

Effect of the Structure of Therapeutic Adenosine Analogues on Stability and Surface Electrostatic Potential of their Complexes with Poly(propyleneimine) Dendrimers

Gorzkiewicz

Appelhans

Boye

et al. 2019

Macromol. Rapid Commun.

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Poly(propyleneimine) glycodendrimers are proposed as nanocarriers for triphosphate forms of anticancer adenosine analogues to improve the efficiency of chemotherapy and to overcome drug resistance mechanisms. This approach has proven successful for fludarabine administration—an autonomous way of cellular entry of a nucleotide–dendrimer noncovalent complex enables an increase in the intracellular accumulation and cytotoxic activity of the active metabolite of the drug. However, the attempt to apply an analogous strategy for clofarabine results in the inhibition of drug activity. To better understand this phenomenon, characterization and comparison of drug‐dendrimer complexes were needed to indicate the differences in their surface properties and the strengths of fludarabine‐dendrimer and clofarabine‐dendrimer interactions. Here, zeta potential measurements, ultrafiltration, and asymmetric flow field‐flow fractionation are applied to determine the surface electrostatic potential and stability of nucleotide–dendrimer formulations. This approach significantly extends the authors’ research on the complexation potential of perfectly branched macromolecules, ultimately explaining previously observed differences and their consequences.

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Section: Zeta Potential Of Ppi Dendrimers and Nucleotide–dendrimer Comentioning

confidence: 98%

Effect of the Structure of Therapeutic Adenosine Analogues on Stability and Surface Electrostatic Potential of their Complexes with Poly(propyleneimine) Dendrimers

Gorzkiewicz

Appelhans

Boye

et al. 2019

Macromol. Rapid Commun.

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“…Recently, our studies showed that cationic poly(propylene imine) dendrimers and hyperbranched poly(ethylene imine)s (PEI) decorated with oligosaccharide shells are suitable materials to complex anionic analyte molecules (e.g., ATP,13, 14 Mant‐ATP,15 Rose Bengal16 and nucleic acids17, 18). Physico‐chemical and molecular modelling studies confirmed the core–shell architectures of these dendritic glycopolymers 19.…”

Section: Number Of Each Type Of Analyte Molecule Complexed Per Macrommentioning

confidence: 99%

“…Thus, the buffer and ionic strength of these complexation conditions significantly influence the complexation capability of the PEI‐OS architectures under the defined conditions. In this context, the different interaction features of dense‐ and open‐shell glycodendrimers with ATP and Mant‐ATP, determined in water and PBS buffer, have been described, wherein the influence of the oligosaccharide architecture and the PBS buffer was evaluated 14. 15…”

Section: Number Of Each Type Of Analyte Molecule Complexed Per Macrommentioning

confidence: 99%

Oligosaccharide Shells as a Decisive Factor for Moderate and Strong Ionic Interactions of Dendritic Poly(ethylene imine) Scaffolds under Shear Forces

Tripp

Appelhans

Striegler

et al. 2014

Chemistry A European J

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For better understanding and improving the non-covalent interactions of dendritic core-shell, we evaluated the interactions of hyperbranched poly(ethylene imine) (PEI) decorated with various oligosaccharide shells with water-soluble B vitamins, an estradiol derivative and pantoprazole. Depending on the different properties of the analyte molecules, dendritic core-shell glyco architectures showed (very) weak, moderate and strong interactions with the analyte molecules. Thus, ionic interactions are the strongest driving force for the formation of host-guest complexes. The core-shell glyco architecture is a necessary prerequisite for stable analyte/PEI complexes; the pure hyperbranched PEI did not show any sufficiently strong interactions with neutral, cationic or anionic analytes under the shear forces applied during ultrafiltration of pure aqueous solution without an adjusted pH. Thus, only robust non-covalent interactions between analytes and the dendritic polyamine scaffold of the glycopolymer structure survive this separation step and allow isolation of stable host-guest complexes in aqueous solution.

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“…In recent studies [19][20][21][22][23], the coupling of mono-and oligosaccharide units has successfully been accomplished. Hence, these dendritic glycoarchitectures have been considered as nanocarriers for drugs [19,20,23]. For instance, dendritic glycoarchitectures based on PEI core [19,23] outlines enhanced biocompatibility and reduced cytotoxicity compared with the bare hyperbranched PEI.…”

Section: Introductionmentioning

confidence: 98%

“…Though an enhancement of biocompatibility can be achieved through the incorporation of water-soluble and biocompatible surface groups in the outer shell of the dendritic architecture consisting of poly(propylene imine) (PPI) dendrimers, poly(amido amine) (PAMAM) dendrimers, hyperbranched poly(ethylene imine) (PEI), and other dendritic scaffolds [13][14][15][16][17][18]. In recent studies [19][20][21][22][23], the coupling of mono-and oligosaccharide units has successfully been accomplished. Hence, these dendritic glycoarchitectures have been considered as nanocarriers for drugs [19,20,23].…”

Section: Introductionmentioning

confidence: 99%

Characterization of oligosaccharide-functionalized hyperbranched poly(ethylene imine) and their complexes with retinol in aqueous solution

Bekhradnia

Naz

Lund

et al. 2015

Journal of Colloid and Interface Science

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Characteristics of complexes between poly(propylene imine) dendrimers and nucleotides

Cited by 13 publications

References 34 publications

Effect of the Structure of Therapeutic Adenosine Analogues on Stability and Surface Electrostatic Potential of their Complexes with Poly(propyleneimine) Dendrimers

Effect of the Structure of Therapeutic Adenosine Analogues on Stability and Surface Electrostatic Potential of their Complexes with Poly(propyleneimine) Dendrimers

Oligosaccharide Shells as a Decisive Factor for Moderate and Strong Ionic Interactions of Dendritic Poly(ethylene imine) Scaffolds under Shear Forces

Characterization of oligosaccharide-functionalized hyperbranched poly(ethylene imine) and their complexes with retinol in aqueous solution

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