Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis

Xiang, Zhiming; Qu, Fufa; Wang, Fuxuan; Li, Jun; Zhang, Yuehuan; Zhou, Yu

doi:10.1016/j.fsi.2014.08.006

Cited by 21 publications

(8 citation statements)

References 32 publications

(43 reference statements)

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“…Contradictory to the observation of Zhang et al . [ 25 ], ficolins, which have been linked to the complement system [ 55 ], have already been detected in molluscs, with transcripts found in the scallop P . maximus [ 53 ] and the C .…”

Section: Resultsmentioning

confidence: 99%

“…Xiang et al . [ 55 ] showed an increased expression of a ficolin-like gene in Crassostrea hongkongensis after the oyster was challenged with microbes, with the recombinant protein being able to agglutinate Escherichia coli K-12 in the presence of Ca 2+ and raising the phagocytic activity of C . hongkongensis haemocytes.…”

Section: Resultsmentioning

confidence: 99%

“…hongkongensis was shown to increase the phagocytic activity of C . hongkongensis haemocytes by acting as an opsonin [ 55 ]. Having these multiple receptors that directly or indirectly (e.g.…”

Section: Resultsmentioning

confidence: 99%

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Transcriptome Analysis of the Sydney Rock Oyster, Saccostrea glomerata: Insights into Molluscan Immunity

et al. 2016

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BackgroundOysters have important ecological functions in their natural environment, acting as global carbon sinks and improving water quality by removing excess nutrients from the water column. During their life-time oysters are exposed to a variety of pathogens that can cause severe mortality in a range of oyster species. Environmental stressors encountered in their habitat can increase the susceptibility of oysters to these pathogens and in general have been shown to impact on oyster immunity, making immune parameters expressed in these marine animals an important research topic.ResultsPaired-end Illumina high throughput sequencing of six S. glomerata tissues exposed to different environmental stressors resulted in a total of 484,121,702 paired-end reads. When reads and assembled transcripts were compared to the C. gigas genome, an overall low level of similarity at the nucleotide level, but a relatively high similarity at the protein level was observed. Examination of the tissue expression pattern showed that some transcripts coding for cathepsins, heat shock proteins and antioxidant proteins were exclusively expressed in the haemolymph of S. glomerata, suggesting a role in innate immunity. Furthermore, analysis of the S. glomerata ORFs showed a wide range of genes potentially involved in innate immunity, from pattern recognition receptors, components of the Toll-like signalling and apoptosis pathways to a complex antioxidant defence mechanism.ConclusionsThis is the first large scale RNA-Seq study carried out in S. glomerata, showing the complex network of innate immune components that exist in this species. The results confirmed that many of the innate immune system components observed in mammals are also conserved in oysters; however, some, such as the TLR adaptors MAL, TRIF and TRAM are either missing or have been modified significantly. The components identified in this study could help explain the oysters’ natural resilience against pathogenic microorganisms encountered in their natural environment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Transcriptome Analysis of the Sydney Rock Oyster, Saccostrea glomerata: Insights into Molluscan Immunity

et al. 2016

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“…Nevertheless, other mollusks display a high number of proteins with a simpler architecture, comprising a signal peptide and the fibrinogen-like domain, often paired with a coiled-coil region of variable length, which may allow their oligomerization, in a similar fashion to collagen in vertebrate ficolins [45]. Single-domain FReDs, which retain significant glycan-binding properties in the Lophotrochozoa [46], underwent a significant expansion in bivalves, where they are often found with hundreds of paralogous gene copies [47] encoding inducible proteins with marked bacteria-agglutinating properties [48,49]. Similar expansions have certainly occurred in other lophotrochozoan phyla, such as brachiopods, even though the functional implications of these events are presently unclear [50].…”

Section: Fred-containing Proteinsmentioning

confidence: 99%

First Insights into the Repertoire of Secretory Lectins in Rotifers

Gerdol

2022

Marine Drugs

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Due to their high biodiversity and adaptation to a mutable and challenging environment, aquatic lophotrochozoan animals are regarded as a virtually unlimited source of bioactive molecules. Among these, lectins, i.e., proteins with remarkable carbohydrate-recognition properties involved in immunity, reproduction, self/nonself recognition and several other biological processes, are particularly attractive targets for biotechnological research. To date, lectin research in the Lophotrochozoa has been restricted to the most widespread phyla, which are the usual targets of comparative immunology studies, such as Mollusca and Annelida. Here we provide the first overview of the repertoire of the secretory lectin-like molecules encoded by the genomes of six target rotifer species: Brachionus calyciflorus, Brachionus plicatilis, Proales similis (class Monogononta), Adineta ricciae, Didymodactylos carnosus and Rotaria sordida (class Bdelloidea). Overall, while rotifer secretory lectins display a high molecular diversity and belong to nine different structural classes, their total number is significantly lower than for other groups of lophotrochozoans, with no evidence of lineage-specific expansion events. Considering the high evolutionary divergence between rotifers and the other major sister phyla, their widespread distribution in aquatic environments and the ease of their collection and rearing in laboratory conditions, these organisms may represent interesting targets for glycobiological studies, which may allow the identification of novel carbohydrate-binding proteins with peculiar biological properties.

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“…It contained a typical signal peptide and a fibrinogen-related domain at the N- and C-terminus, respectively, but no additional ficolin collagen-like domain. Ch FCN mRNA transcripts increased under microbial infections and the recombinant Ch FCN protein could bind and agglutinate microorganisms, and enhance the phagocytosis [ 91 ].…”

Section: Canonical Carbohydrates Recognition Protein In Molluscsmentioning

confidence: 99%

Pathogen-Derived Carbohydrate Recognition in Molluscs Immune Defense

Wang

Song

Wang

et al. 2018

IJMS

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Self-nonself discrimination is a common theme for all of the organisms in different evolutionary branches, which is also the most fundamental step for host immune protection. Plenty of pattern recognition receptors (PRRs) with great diversity have been identified from different organisms to recognize various pathogen-associated molecular patterns (PAMPs) in the last two decades, depicting a complicated scene of host-pathogen interaction. However, the detailed mechanism of the complicate PAMPs–PRRs interactions at the contacting interface between pathogens and hosts is still not well understood. All of the cells are coated by glycosylation complex and thick carbohydrates layer. The different polysaccharides in extracellular matrix of pathogen-host are important for nonself recognition of most organisms. Coincidentally, massive expansion of PRRs, majority of which contain recognition domains of Ig, leucine-rich repeat (LRR), C-type lectin (CTL), C1q and scavenger receptor (SR), have been annotated and identified in invertebrates by screening the available genomic sequence. The phylum Mollusca is one of the largest groups in the animal kingdom with abundant biodiversity providing plenty of solutions about pathogen recognition and immune protection, which might offer a suitable model to figure out the common rules of immune recognition mechanism. The present review summarizes the diverse PRRs and common elements of various PAMPs, especially focusing on the structural and functional characteristics of canonical carbohydrate recognition proteins and some novel proteins functioning in molluscan immune defense system, with the objective to provide new ideas about the immune recognition mechanisms.

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Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis

Cited by 21 publications

References 32 publications

Transcriptome Analysis of the Sydney Rock Oyster, Saccostrea glomerata: Insights into Molluscan Immunity

Transcriptome Analysis of the Sydney Rock Oyster, Saccostrea glomerata: Insights into Molluscan Immunity

First Insights into the Repertoire of Secretory Lectins in Rotifers

Pathogen-Derived Carbohydrate Recognition in Molluscs Immune Defense

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