Characterisation of two rat mammary tumour models for breast cancer research by gene expression profiling

Marxfeld, Heike; Staedtler, Frank; Harleman, Johannes H.

doi:10.1016/j.etp.2006.05.003

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…In addition, it is important to develop good in vivo models to analyze each step of the metastatic spread. Rat mammary adenocarcinoma MTLn3 cells have been identified on the basis of gene profiling as a suitable model to study breast cancer progression and treatment (Neri and Nicolson, 1981;Marxfeld et al, 2006) and to follow actin cytoskeleton remodeling in live carcinoma cells (Lorenz et al, 2004). The epidermal growth factor receptor (EGFR) is often over-expressed in breast cancer, resulting not only in uncontrolled cell proliferation (Citri and Yarden, 2006;Normanno et al, 2006) but also in increased tumor cell motility and invasion (Condeelis and Segall, 2003).…”

Section: Introductionmentioning

confidence: 99%

p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation

et al. 2011

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We have recently shown that the adaptor protein p140Cap regulates tumor properties in terms of cell motility and growth. Here, by using the highly metastatic rat adenocarcinoma cell line MTLn3-epidermal growth factor receptor (EGFR), we assess the role of p140Cap in metastasis formation. Orthotopic transplantation of MTLn3-EGFR cells over-expressing p140Cap in Rag2À/À mice resulted in normal primary tumor growth compared with the controls. Strikingly, p140Cap over-expression causes an 80% inhibition in the number of lung metastases. p140Cap over-expressing cells display a 50% reduction in directional cell migration, an increased number and size of focal adhesions, and a strong impairment in the ability to invade in a 3D matrix. p140Cap overexpression affects EGFR signaling and tyrosine phosphorylation of cortactin in response to EGF stimulation. Intriguingly, p140Cap associates with cortactin via interaction with its second proline-rich domain to the cortactin SH3 domain. The phosphomimetic cortactin tyrosine 421 mutant rescues migration and invasive properties in p140Cap over-expressing cells. Taken together, these data demonstrate that p140Cap suppresses the invasive properties of highly metastatic breast carcinoma cells by inhibiting cortactin-dependent cell motility.

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Section: Introductionmentioning

confidence: 99%

p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation

et al. 2011

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“…Marxfeld et al [24] examined differences in gene expression between MTLn3-induced tumors and non-cancerous control mammary tissue. MTC or MTLn2-induced tumors were not studied.…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15][16][17][18][19][20][21][22][23]. Two studies have examined differences in gene expression between MTLn3 cells and MTC or normal cells in culture or in vivo [18,24]. However, there has been no systematic approach to studying differences in protein expression between the sublines.…”

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confidence: 99%

Biomarkers of metastatic potential in cultured adenocarcinoma clones

Dabbous

Jefferson

Haney

et al. 2010

Clin Exp Metastasis

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Two-dimensional isoelectric focusing and gel electrophoresis followed by mass spectrometry were used to detect, measure, and identify changes in protein expression correlated with differences in the metastatic potential of cultured rat mammary adenocarcinoma cells. MTC is a non-metastatic cell clone derived from a primary tumor. MTLn2 and MTLn3 are low and high metastatic potential cell clones derived from lung metastases of the primary tumor. A total of 1,500 proteins was detected. The patterns of protein expression of MTLn2 and MTLn3 cells were similar. Only five spots had a threefold or greater statistically significant difference in staining intensity between MTLn2 and MTLn3 cells, whereas 70 spots differed between MTC and MTLn3 cells. Twenty spots were selected for further study, ten that had a positive correlation of staining intensity with metastatic potential and ten that had a negative (inverse) correlation. Of the 17 unique proteins that were identified, five have often been cited as tumor biomarkers. These included the positive biomarkers nucleophosmin (NPM) and 14-3-3 protein sigma and the negative biomarkers raf kinase inhibitor protein (RKIP), peroxiredoxin-2, and galectin-1. The only identified protein that was markedly higher in MTLn3 cells than in the less-metastatic MTLn2 cells was 14-3-3 protein sigma. The results indicate that increased metastatic potential is associated with positive and negative changes in expression of particular proteins. Proteins that are positively correlated with metastatic potential may prove more useful as clinical biomarkers, but those with negative correlations may still provide useful information about underlying mechanisms of metastatic spread.

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“…Tumoral cell lines have proven to be suitable in vitro cellular models for breast cancer research. However, a proper characterization of these cell lines is mandatory to ensure that they accurately recapitulate the processes and mechanism features under research [Marxfeld et al, 2006;Louzada et al, 2012].…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Assessment of the Rat Cell Line CLS-ACI-1: An in vitro Cell Model for <b><i>Mycn</i></b> Overexpression

Meles

Adega²,

Castro³

et al. 2015

Cytogenet Genome Res

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Breast cancer is a complex and heterogeneous disease, and the establishment of cell models in order to properly study the disease at the molecular and cellular level is of utmost importance. Here, we present the cytogenetic characterization and gene expression analysis of the tumoral mammary rat cell line CLS-ACI-1. The use of banding and molecular cytogenetic techniques allowed the description of the complex CLS-ACI-1 karyotype and the identification of breakpoints in clonal chromosome rearrangements. Moreover, a Mycn and Erbb2 comparative expression analysis by RT-qPCR was performed, revealing a high expression level of Mycn in CLS-ACI-1 cells. Moreover, a considerable number of putative mutated genes and chromosome alterations detected through cytogenetic analysis seem to be in the MYCN biological network. Therefore, the CLS-ACI-1 cell line is presented as a promising cell model for the study of the role of MYCN in breast cancer and also as a tool for developing appropriate cancer therapies, namely for Mycn targeting.

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Characterisation of two rat mammary tumour models for breast cancer research by gene expression profiling

Cited by 8 publications

References 39 publications

p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation

p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation

Biomarkers of metastatic potential in cultured adenocarcinoma clones

Cytogenetic Assessment of the Rat Cell Line CLS-ACI-1: An in vitro Cell Model for <b><i>Mycn</i></b> Overexpression

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