Characterisation of the secreted apyrase family of Heligmosomoides polygyrus

Berkachy, Rita; Smyth, Danielle J.; Schnoeller, Corinna; Harcus, Yvonne; Maizels, Rick M.; Selkirk, Murray E.; Gounaris, Kleoniki

doi:10.1016/j.ijpara.2020.07.011

Cited by 6 publications

(7 citation statements)

References 53 publications

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“…This effect could be replicated by administration of HES in the absence of infection [134], and correlated with reduced IL-33-dependent ILC2 activation [48,135]. Multiple pathways have been proposed or demonstrated for the IL-33-suppressive effects of HES, most recently with our identification of 2 families of IL-33-suppressive proteins secreted by the parasite [38,49,50,136,137] (Figure 2).…”

Section: Helminth Modulation Of the Il-33 Pathwaymentioning

confidence: 77%

IL-33: A central cytokine in helminth infections

McSorley

Smyth

2021

Seminars in Immunology

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Section: Helminth Modulation Of the Il-33 Pathwaymentioning

confidence: 77%

IL-33: A central cytokine in helminth infections

McSorley

Smyth

2021

Seminars in Immunology

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“…A unique apyrase has also been identified in the ES of females that developed in the colitis microenvironment. Apyrases are enzymes produced by various parasitic worm species [ 34 , 35 , 36 ]. A potential function of the apyrases produced by nematodes is the hydrolysis of extracellular ATP.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Intestinal Inflammation on Nematode’s Excretory–Secretory Proteome

Maruszewska-Cheruiyot,

Szewczak,

Krawczak-Wójcik

et al. 2023

IJMS

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Parasitic nematodes and their products are promising candidates for therapeutics against inflammatory bowel diseases (IBD). Two species of nematodes, the hookworm Necator americanus and the whipworm Trichuis suis, are being used in clinical treatment trials of IBD referred to as “helminth therapy”. Heligmosomoides polygyrus is a well-known model for human hookworm infections. Excretory–secretory (ES) products of H. polygyrus L4 stage that developed during colitis show a different immunomodulatory effect compared to the ES of H. polgyrus from healthy mice. The aim of the study was to evaluate excretory–secretory proteins produced by H. polygyrus L4 stage males and females that developed in the colitic milieu. Mass spectrometry was used to identify proteins. Blast2GO was used to investigate the functions of the discovered proteins. A total of 387 proteins were identified in the ES of H. polygyrus L4 males (HpC males), and 330 proteins were identified in the ES of L4 females that developed in the colitic milieu (HpC females). In contrast, only 200 proteins were identified in the ES of L4 males (Hp males) and 218 in the ES of L4 females (Hp females) that developed in control conditions. Most of the proteins (123) were detected in all groups. Unique proteins identified in the ES of HpC females included annexin, lysozyme-2, apyrase, and galectin. Venom allergen/Ancylostoma-secreted protein-like, transthyretin-like family proteins, and galectins were found in the secretome of HpC males but not in the secretome of control males. These molecules may be responsible for the therapeutic effects of nematodes in DSS-induced colitis.

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“…The presence of enzymes has been described in a wide range of eukaryotic organisms, from yeasts to mammals, in addition to nematodeos e trematodeos. [5][6][7][8][9][10] In S. mansoni, a broad expression of ATP diphosphohydrolase at all stages of the parasite life cycle has been described. 11 Previous studies demonstrated evidence of antigenic properties of S. mansoni ATP diphosphohydrolase, 12,13 and the enzymes have been identified as candidates for S. mansoni virulence factors, which may contribute to schistosomiasis's pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

“…ATP‐ diphosphohydrolases (EC 3.6.1.5) are enzymes calcium and magnesium‐dependent and hydrolase a variety of nucleoside tri‐ and diphosphates. The presence of enzymes has been described in a wide range of eukaryotic organisms, from yeasts to mammals, in addition to nematodeos e trematodeos 5–10 . In S. mansoni , a broad expression of ATP diphosphohydrolase at all stages of the parasite life cycle has been described 11 .…”

Section: Introductionmentioning

confidence: 99%

Expression of SmATPDases 1 and 2 in Schistosoma mansoni eggs favours IL‐10 production in infected individuals

Gomides,

de Souza,

de Figueiredo

et al. 2023

Parasite Immunology

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A role of IL‐10 is down‐regulating T‐cell responses to schistosome antigens. Since SmATPDases can be correlated to modulation of the immune response, we evaluated the expression of enzymes in S. mansoni eggs. Faecal samples were collected from 40 infected individuals to detect coding regions of the SmATPDases. The cytokines were measured in supernatants of PBMC. The analysis was performed by the global median determination and set up high producers (HP) of cytokines. Six individuals expressed SmATPDase1, six expressed SmATPDase2 and six expressed both enzymes. The group who expressed only SmATPDase1 showed a high frequency of IFN‐γ, TNF IL‐4 HP; individuals who expressed only SmATPDase2 showed a high frequency of IFN‐γ, IL‐6 and IL‐4 HP; and individuals who expressed both enzymes showed a high frequency of IL‐10 HP. The comparison of the IFN‐γ/IL‐10 ratio presented higher indices in the group who had SmATPDase 2 expression than those who had the expression of both enzymes. The positive correlation between infection intensity and IL‐10 levels remained only in the positive SmATPDase group. The IL‐10 is the only cytokine induced by the expression of both enzymes. Our data suggest that the expression of both enzymes seems to be a factor that modulates the host immune response by inducing high IL‐10 production.

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Characterisation of the secreted apyrase family of Heligmosomoides polygyrus

Cited by 6 publications

References 53 publications

IL-33: A central cytokine in helminth infections

IL-33: A central cytokine in helminth infections

The Impact of Intestinal Inflammation on Nematode’s Excretory–Secretory Proteome

Expression of SmATPDases 1 and 2 in Schistosoma mansoni eggs favours IL‐10 production in infected individuals

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