Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM

Pérez, Carlos; Moreno, Sandra; Summerfield, Artur; Doménech, Nieves; Álvarez, Belén; Correa, Carlos; Alonso, Fernando; Ezquerra, Ángel; Domı́nguez, Javier; Revilla, Concepción

doi:10.1016/j.jim.2007.01.003

Cited by 17 publications

(10 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B cell lineage subpopulations in IPP do not resemble bone marrow and have a phenotype similar to secondary lymphoid organs Some reports (31,43,(45)(46)(47)(48) and our recent unpublished observations indicate that the development of porcine B cell lineage cells can be monitored by stable MHC-II expression, decreasing expression of CD172a (SWC3), and increasing expression of CD2, CD25, and CD45RC. To characterize whether cells in IPP have the same or a different phenotype from bone marrow B cell lineage cells, we analyzed these tissues together with blood in 36-d-old germ-free animals and also in animals of the same age in which surgically constructed isolated ileal loops were made (Fig.…”

Section: Ipp That Do Not Have Access To Colonizing Bacteria or Food Amentioning

confidence: 94%

Ileal Peyer’s Patches Are Not Necessary for Systemic B Cell Development and Maintenance and Do Not Contribute Significantly to the Overall B Cell Pool in Swine

Šinkora

Štěpánová

Butler

et al. 2011

The Journal of Immunology

View full text Add to dashboard Cite

Based on studies of sheep, ileal Peyer’s patches (IPP) have been regarded as a type of primary lymphoid tissue similar to the bursa of Fabricius in chicken. Because bursectomy results in B cell deficiency, we wondered whether resection of the IPP of piglets would have a similar effect. Comparison of IPP-resected, surgical shams and untreated germ-free piglets, all of which were later colonized with a defined commensal flora, demonstrated that resection of the IPP did not alter the level and phenotype of B and T cells in lymphoid tissues and the blood 10 wk after surgery. Additionally, colonization of IPP caused a shift from the fetal type of lymphocyte distribution to the adult type that is characterized by prevalence of B cells, with many of them representing IgA+ switched B cells or displaying a more mature CD2−CD21+ and CD2−CD21− phenotype. Moreover, colonization leads to appearance of effector CD4+CD8+ αβ T helper and CD2+CD8− γδ T cells. Comparison of germ-free with colonized pigs and experiments utilizing surgical transposition of jejunal Peyer’s patch into terminal ileum or construction of isolated ileal loops indicated that lymphocyte development in IPP is dependent on colonization. Although our studies confirmed higher mitotic and apoptotic rates in IPP, they failed to identify any cell populations that resemble developing B lineage cells in the bone marrow. These results indicate that porcine IPP are not required for systemic B cell generation or maintenance, but they are secondary lymphoid tissue that appears important in immune responses to colonizing bacteria.

show abstract

Section: Ipp That Do Not Have Access To Colonizing Bacteria or Food Amentioning

confidence: 94%

Ileal Peyer’s Patches Are Not Necessary for Systemic B Cell Development and Maintenance and Do Not Contribute Significantly to the Overall B Cell Pool in Swine

Šinkora

Štěpánová

Butler

et al. 2011

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…In the swine, the stem cell activity of CD117 + cells have been previously evaluated by using SCF to activate their stem cell potential to differentiate into various cellular lineages (20,21). Although a swine CD117 Mab has been developed and characterized (24), the species specificity of the Mab and xenotransplantation of CD117 + cells have not been reported. They checked in vitro development of erythroid and myeloid cells from Mab-enriched cells, but they could not detect lymphoid cell development.…”

Section: Discussionmentioning

confidence: 99%

Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

Ohshima¹,

Mori²,

Shigenari³

et al. 2014

BST

View full text Add to dashboard Cite

“…Up to now, numerous specific surface antigens are known in human and murine hematopoietic stem cells [11], but few are known for LSPCs. Hence, we selected six possible stem cell antigens: CD117, c-Met, Sca-1, CD90, CD133 and CD49f [12][13][14][15][16][17]. Among these markers, parts in defining LSPCs remain controversial at present [14,15,[18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver Cells as a Source of Liver Stem/Progenitor Cells

Liu

Ren

Dou

2010

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Although the stem cells are commonly isolated by FACS or MACS, they are very expensive and these is no specific marker for liver stem/progentior cells (LSPCs). This paper applied a convenient and efficient method to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation (PDGC) from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence (DTDA). Flow cytometric analysis revealed more than half of the purified FLCs expressed alternative markers of LSPCs (CD117, c-Met, Sca-1, CD90, CD49f and CD133). In other words, the purified FLCs were heterogeneous. Therefore, they were sequentially layered into six fractions by Percoll continuous gradient centrifugation (PCGC). Both CD133 and CD49f expressed decreasingly from fraction 1 to 6. In fraction 1 and 2, about 85% FLCs expressed CD133, which were revealed to be LSPCs by high expressions of AFP and CK-19, low expressions of G-6-P and ALB. To conclude, the purity of CD133(+) LSPCs enriched by combination of PDGC, DTDA and PCGC is close to that obtained by MACS. This study will greatly contribute to two important biological aspects: liver stem cells isolation and liver cell therapy.

show abstract

Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM

Cited by 17 publications

References 26 publications

Ileal Peyer’s Patches Are Not Necessary for Systemic B Cell Development and Maintenance and Do Not Contribute Significantly to the Overall B Cell Pool in Swine

Ileal Peyer’s Patches Are Not Necessary for Systemic B Cell Development and Maintenance and Do Not Contribute Significantly to the Overall B Cell Pool in Swine

Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver Cells as a Source of Liver Stem/Progenitor Cells

Contact Info

Product

Resources

About